Team:Uppsala/betacarotene

From 2013.igem.org

(Difference between revisions)
 
(23 intermediate revisions not shown)
Line 1: Line 1:
-
{{Team:Uppsala/standard-style}}
+
{{Team:Uppsala/betacarotene-style}}
-
{{Team:Uppsala/modelling-style}}
+
{{Team:Uppsala/standard-style-no-frame}}
<html>
<html>
Line 9: Line 9:
<link rel="shortcut icon" href="favicon.ico" type="image/x-icon">
<link rel="shortcut icon" href="favicon.ico" type="image/x-icon">
<link rel="stylesheet" type="text/css" href="css/style.css">
<link rel="stylesheet" type="text/css" href="css/style.css">
 +
<link rel="stylesheet" type="text/css" href="css/biyo.css">
<style type="text/css">
<style type="text/css">
</style>
</style>
 +
 +
<script type="text/javascript">
 +
/*Acts once the document is loaded*/ $(document).ready(function() { /*Move the tn-main-wrap as child of <body>*/ $("#tn-main-wrap-wrap").prependTo($("body")); /*Move the menubars in the tn-main-wrap*/ $("#menubar.left-menu").appendTo($("#tn-main-wrap")); $("#menubar.right-menu").appendTo($("#tn-main-wrap"));
 +
/*Spoiler JS*/ $(".tn-spoiler div").slideUp(); $(".tn-spoiler a").click(function(e) { e.preventDefault(); $(".tn-spoiler-active").removeClass("tn-spoiler-active"); $(this).parent().addClass("tn-spoiler-active"); $(".tn-spoiler").not(".tn-spoiler-active").children("div").slideUp(); $(".tn-spoiler-active").children("div").slideToggle(); });
 +
/*Add favicon*/ $("link[rel='shortcut icon']").remove(); $("head").append("<link rel='shortcut icon' type='image/png' href='https://static.igem.org/mediawiki/2013/6/6d/Uppsalas-cow-con.png'>"); });
 +
</script>
 +
 +
<link href="https://2013.igem.org/Team:Uppsala/lightbox-css-code.css?action=raw&ctype=text/css" type="text/css" rel="stylesheet">
 +
 +
<script src="https://2013.igem.org/Team:Uppsala/jquery-code.js?action=raw&ctype=text/javascript" type="text/javascript"></script>
 +
 +
<script src="https://2013.igem.org/Team:Uppsala/lightbox-code.js?action=raw&ctype=text/javascript" type="text/javascript"></script>
</head>
</head>
Line 26: Line 39:
<!-- Contains sub-logo picture -->
<!-- Contains sub-logo picture -->
-
<div id="igem_logo"><a href="https://2013.igem.org/Main_Page"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/5/59/IGEMlogo.png"></a></div>
+
<div id="igem_logo"><a href="https://2013.igem.org/Team:Uppsala"><img class="igem_logo" src="https://static.igem.org/mediawiki/2013/e/e0/Uppsala2013_IGEMlogo.png"></a></div>
-
<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/9/9c/Uppsala_IGEM_log_blue.png"></a></div>
+
<div id="igem"><a href="https://2013.igem.org/Main_Page"><img class="igem" src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_Uppsala_IGEM_log_blue.png"></a></div>
 +
 
 +
<img class="slogan" src="https://static.igem.org/mediawiki/2013/a/ad/Uppsala2013_Header.png">
</div>  
</div>  
Line 43: Line 58:
<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/a/aa/Uppsala2013_Project.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/project" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/a/aa/Uppsala2013_Project.png"></a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/overview">Overview</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/chassi">Chassi</a>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi">Chassi</a>
<ul>
<ul>
Line 49: Line 64:
<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/promoters">Promoters</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/reporter-genes">Reporter genes</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/reporter-genes">Reporter genes</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/affinity-tags">Toxin-antitoxin system</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/toxin-antitoxin-system">Toxin-antitoxin system</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/vectors">Vectors</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/signal-peptide">Signal peptide</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/signal-peptide">Signal peptide</a></li>
Line 56: Line 71:
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                 <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a>
                                                     <ul>
                                                     <ul>
-
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">P-coumaric acid</a></li>
+
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
                                                                 <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li>
Line 62: Line 77:
                                                                 <li><a  
                                                                 <li><a  
href="https://2013.igem.org/Team:Uppsala/saffron">Saffron</a></li>
href="https://2013.igem.org/Team:Uppsala/saffron">Saffron</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/astraxantin">Astaxanthin</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/astaxanthin">Astaxanthin</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/zeaxantin">Zeaxanthin</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">Zeaxanthin</a></li>
</ul></li>
</ul></li>
</li>  
</li>  
Line 69: Line 84:
href="https://2013.igem.org/Team:Uppsala/miraculin">Miraculin</a></li>
href="https://2013.igem.org/Team:Uppsala/miraculin">Miraculin</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chromoproteins">Chromoproteins</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chromoproteins">Chromoproteins</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/safety">Safety experiment</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/safety-experiment">Safety experiment</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/result">Result</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/results">Results</a></li>
Line 77: Line 92:
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/resveratrol-pathway">Resveratrol pathway </a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li>
 +
 +
<li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li>
</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li>
Line 87: Line 104:
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
+
                                                <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li>
 +
</ul></li>
</ul></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/hp" id="list_type3"><img class="nav-text"      src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_HP.png"></a>
+
<li><a href="https://2013.igem.org/Team:Uppsala/human-practice" id="list_type3"><img class="nav-text"      src="https://static.igem.org/mediawiki/2013/b/b8/Uppsala2013_HP.png"></a>
<ul>
<ul>
-
<li><a href="https://2013.igem.org/Team:Uppsala/Yoghurtproducts">Yoghurt products</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/yoghurt">Yoghurt +</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/synbioday">SynBio day</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/synbioday">SynBioDay</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/safety">Bioethics and safety</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/biosafety-and-ethics">Biosafety and ethics</a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/society">Society and outreach</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li>
-
<li><a href="https://2013.igem.org/Team:Uppsala/highschool-outreach">High school outreach</a></li>
+
                                                <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li>
-
                                                <li><a href="https://2013.igem.org/Team:Uppsala/outreach">Media outreach</a></li>
+
<li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li>
 +
<li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li>
</ul></li>
</ul></li>
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
<li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li>  
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a>
                                     <ul>
                                     <ul>
-
                                         <li><a href="https://2013.igem.org/Team:Uppsala/protocol">Protocol</a></li>
+
                                         <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li>
-
                                         <li><a href="https://2013.igem.org/Team:Uppsala/notebook">Lab journal</a></li>
+
                                         <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li>
                                     </ul></li>
                                     </ul></li>
</ul>
</ul>
Line 108: Line 129:
</div>
</div>
 +
<div id="main_content"> <!-- Put content here -->
<div id="main_content"> <!-- Put content here -->
-
+
<h1 class="main-title-left">β-carotene</h1>
-
        <div id="envelope">  
+
       
-
+
        <img class="metabolic-pic" src="https://static.igem.org/mediawiki/2013/0/00/Uppsala2013_beta-caroteneSymbol.png">
-
<div id="molecule"> <div class="head"> <h1 > Molecule X </h1> </div>
+
       
-
X
+
        <div id="clear"></div>
-
</div>
+
-
<div id="methods"> <div class="head"> <h1> Methods </h1> </div>
+
<p>β-carotene is a common carotenoid that derives from the xanthophyll group known for its characteristic orange color. β-carotene can be found in fruits and vegetables, for example carrots. β-carotene is a provitamin to vitamin A, which means that during the right circumstances β-carotene can be catalyzed further to produce vitamin A. Vitamin A has several important health aspects which includes skin, mucus membrane and most common to people a good effect on eyes and vision. Studies have shown that the carotenoid can prevent aging of the brain and stimulate it to keep its capability of memory when a high ascorbic acid concentration also is present <sup><a href="#ref1">[1]</a></sup>. The health aspects were an important part of our choice to produce β-carotene.</p>
-
Methods
+
-
</div>
+
        <a href="https://static.igem.org/mediawiki/2013/f/f4/Uppsala2013_Beta-carotene-molecule.png" data-lightbox="roadtrip"><img class="molecule" src="https://static.igem.org/mediawiki/2013/f/f4/Uppsala2013_Beta-carotene-molecule.png"></a>
 +
<a id="b1"></a><h1>Methods</h1>
 +
<p>The gene CrtY that is responsible for translating the protein lycopene cyclase was obtained from the zeaxanthin operon provided by Slovenia iGEM team 2010. Production of β-carotene is initiated through the catalytic reaction done by the enzyme lycopene cyclase which uses the precursor <a href="https://2013.igem.org/Team:Uppsala/lycopene">lycopene.</a></p>
 +
 +
        <a href="https://static.igem.org/mediawiki/2013/d/d2/Uppsala2013_Beta-caroten-plasmid.png" data-lightbox="roadtrip"><img class="methods-pic" src="https://static.igem.org/mediawiki/2013/d/d2/Uppsala2013_Beta-caroten-plasmid.png"></a>
-
<div id="results">
+
<h1>Results </h1>
 +
<p>Beta carotene production was proven through the production of our operon containing <a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">zeaxanthin.</a> Since production of zeaxanthin relies on beta carotene as a precursor we could simultaneously prove that we successfully had produced both zeaxanthin and beta carotene through liquid-liquid separation and spectrophotometry <a href="https://2013.igem.org/Team:Uppsala/zeaxanthin">(read spectrophotometry of zeaxanthin for more results)</a></p>
-
<div class="head"> <h1> Results </h1> </div>
+
<!--
-
Results
+
<h1>Results (Bio-bricks)</h1>
 +
<p>We realized that there were a lot of components in the carotenoid pathway that we were in need of in the beginning of the project that were missing in the registry. Therefore after obtaining the zeaxanthin operon from Slovenias iGEM team 2010 we directly proceeded with isolating of the genes as basic components in order to send them to the registry. </p>
-
</div>  
+
-->
-
</div>
+
<h1>References</h1>
-
+
-
</div> <!-- main_ontent ends -->  
+
<p><a id="ref1">[1]</a>Perrig WJ, Perrig P, Stähelin HB Institute of Psychology, University of Berne, Switzerland. The relation between antioxidants and memory performance in the old and very old, J Am Geriatr Soc. 1997 Jun;45(6):718-24.</p>
 +
 
 +
</div> <!-- main_content ends -->  
 +
       
<div id="bottom-pic">
<div id="bottom-pic">
-
<img class="bottom-pic" src="https://static.igem.org/mediawiki/2013/a/aa/Bottom_picture.png">
+
</div>
</div>

Latest revision as of 15:21, 28 October 2013

β-carotene

β-carotene is a common carotenoid that derives from the xanthophyll group known for its characteristic orange color. β-carotene can be found in fruits and vegetables, for example carrots. β-carotene is a provitamin to vitamin A, which means that during the right circumstances β-carotene can be catalyzed further to produce vitamin A. Vitamin A has several important health aspects which includes skin, mucus membrane and most common to people a good effect on eyes and vision. Studies have shown that the carotenoid can prevent aging of the brain and stimulate it to keep its capability of memory when a high ascorbic acid concentration also is present [1]. The health aspects were an important part of our choice to produce β-carotene.

Methods

The gene CrtY that is responsible for translating the protein lycopene cyclase was obtained from the zeaxanthin operon provided by Slovenia iGEM team 2010. Production of β-carotene is initiated through the catalytic reaction done by the enzyme lycopene cyclase which uses the precursor lycopene.

Results

Beta carotene production was proven through the production of our operon containing zeaxanthin. Since production of zeaxanthin relies on beta carotene as a precursor we could simultaneously prove that we successfully had produced both zeaxanthin and beta carotene through liquid-liquid separation and spectrophotometry (read spectrophotometry of zeaxanthin for more results)

References

[1]Perrig WJ, Perrig P, Stähelin HB Institute of Psychology, University of Berne, Switzerland. The relation between antioxidants and memory performance in the old and very old, J Am Geriatr Soc. 1997 Jun;45(6):718-24.