A start of a new week but the middle of our experiment. Digestion includes PSB1C3 from our iGEM part. Through gel electrophoresis we can confirm that we are on the right track and via gel extraction we are able to extract our PSB1C3 vector. Unfortunately, the OD value showed that our plasmid concentration is too low! We are not entirely sure what went wrong, is it that we did not follow the protocol correctly? Or is there something wrong with the kits? :/
Tuesday
Repeating yesterdays experiment, we have failed once again We have tried kits from different labs but the results displayed are very much the same. Though the photo of the gel looks beautiful but the concentration is still too low!
What do we dooo……?!
Wednesday
For the past few days we have been doing digestion and gel electrophoresis like a mad dog. Today, we have dedicated ourselves to plasmid extraction! To learn from our lesson, this time we extracted an entire rack so that we will have sufficient PSB1C3 vector for future use.
Thursday
Our experiment is running downhill again!! We are still trying various ways to put things on track, continuously running gel electrophoresis and cutting gel and running gel electrophoresis and cutting gel and asking our advisors as well as seniors, what may go wrong?! But the concentration is still very low…
Our GFP generator has arrived from the iGEM foundation. Still nothing grew, not a tint of bacterium We have again encountered another problem.
Doesn't matter! Never give up! We will continue to try different ways to make it work!
Friday
After our direction was set and our goal was clear, we started to construct our iGEM webpage, filling in related details and keeping our records up to date.
Saturday
In order to save time, we did some basic preparations for the experiment before all the parts were received. Preparations include primer design, preparing LB plates, making competent cells etc. so when the biobrick is received the actual experiment may start immediately.
Sunday
In order to save time, we did some basic preparations for the experiment before all the parts were received. Preparations include primer design, preparing LB plates, making competent cells etc. so when the biobrick is received the actual experiment may start immediately.
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