User contributions

From 2013.igem.org

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• 00:51, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 ‎ (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3) (top)
• 00:48, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 ‎ (→2 - Ligation of pSB1C3 and Pndh*) (top)
• 00:47, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 ‎ (→2 - Ligation of pSB1C3 and Pndh*)
• 00:45, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/1 ‎ (→1 - Digestion of pSB1C3 plasmid and PCR products : Pndh* by EcoRI/ PstI)
• 00:42, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/28 ‎ (→Lab work)
• 00:36, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/28 ‎ (→Lab work)
• 00:31, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/27 ‎ (→Lab work) (top)
• 00:29, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/26 ‎ (→Lab work) (top)
• 00:25, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/25 ‎ (→Labwork) (top)
• 00:22, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/24 ‎ (→Lab work) (top)
• 00:16, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/21 ‎ (→Lab work) (top)
• 00:04, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/20 ‎ (→Notebook : June 20) (top)
• 00:02, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/19 ‎ (→Notebook : June 19) (top)
• 00:01, 5 October 2013 (diff | hist) Team:Paris Saclay/Notebook/June/18 ‎ (→Notebook : June 18) (top)
• 23:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 ‎ (→Saturday 20th April :) (top)
• 23:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 ‎ (→Week end Brainstorming : 20&21 April 2013)
• 23:53, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/April/20 ‎ (→Notebook : April 20)
• 17:47, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→1 - Results of liquid culture of Pfnr-RBS-Amil CP-Term in pSB1C3 in aerobic and anaerobic conditions)
• 17:44, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/31 ‎ (→1 - Results of culture of Pfnr with RBS-LacZ-Term in PSB1K3, Pfnr with RBS-LacZ-Term in PSB1C3 and NarK with RBS-LacZ-term in PSB3K3 in aerobic conditions)
• 17:42, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→7 - Electrophoresis of PCR Colony of Pndh* with RBS_AmilCP-Term in DH5α strain)
• 17:42, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→6 - Transformation of ligation of Pfnr with RBS-LacZ-Term in pSB3K3 and PnarK with RBS-LacZ-Term in pSB3K3 in DH5α strain)
• 17:41, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→7 - Electrophoresis of PCR Colony of Pfnr with RBS_AmilCP-Term in DH5α strain)
• 17:39, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→6 - Transformation of ligation of Pfnr with RBS-LacZ-Term in pSB3K3 and PnarK with RBS-LacZ-Term in pSB3K3 in DH5α strain)
• 17:37, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→Notebook : August 30)
• 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 ‎ (→1 - Culture of BBa_I732017, BBa_K592009)
• 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 ‎ (→2 - Culture of BBa_K1155000)
• 17:04, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 ‎ (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3)
• 17:03, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/July/3 ‎ (→1 - Colony PCR of BBa_K1155000 to check good insertion of Pndh* in pSB1C3)
• 17:01, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→7 - Electrophoresis to check the digestion of BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI/PstI and plasmids already digested by SpeI and after digested by PstI)
• 16:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→2 - PCR Colony of strain DH5α containing plasmid pSB1C3 with Pndh* and RBS_AmilCP-Term)
• 16:53, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→6 - Electrophoresis to check the digestion of BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006 by SpeI and EcoRI/SpeI)
• 16:50, 4 October 2013 (diff | hist) Team:Paris Saclay/Primers ‎ (→Primers)
• 16:49, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→2 - Colony PCR of Pfnr with RBS_AmilCP-Term in DH5α)
• 16:45, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Purification of colonies: NirB with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-AmilCP-Term in pSB1C3 by streaking colonies in aerobic or anaerobic conditions)
• 16:43, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Purification of colonies: NirB with RBS-LacZ-Term in pSB1C3, Pfnr with RBS-Amil CP-Term in pSB1C3 by streaking colonies in aerobic or anaerobic conditions)
• 16:16, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/30 ‎ (→1 - Results of liquid culture of Pfnr-RBS-Amil CP-Term in pSB1C3 in aerobic and anaerobic conditions)
• 16:03, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Colony PCR of FNR, RBS-FNR and RBS-BphR2 in DH5α strain)
• 16:01, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/September/12 ‎ (→1 - Extraction of BBa_K1155000, BBa_K1155001, BBa_K1155002, BBa_K1155003, BBa_K1155004) (top)
• 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Colony PCR of FNR, RBS-FNR and RBS-BphR2 in DH5α)
• 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→3 - Gel purification of the digestion of BBa_J04450 by PstI/SpeI)
• 15:58, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→2 - Electrophoresis to check the digestion of BBa_J04450 by EcoRI/PstI)
• 15:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Digestion of BBa_J04450 by EcoRI/PstI)
• 15:57, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→6 - Electrophoresis to check the gel purification of the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
• 15:56, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→5 - Gel purification of the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
• 15:56, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→4 - Electrophoresis to check the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
• 15:55, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→4 - Electrophoresis to check the digestion of BBa_K1155000 by PstI/SpeI, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
• 15:51, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→1 - Purification of colony transformed with ligation : NirB with RBS-LacZ-Term in PSB1C3, Pfnr with RBS-Amil CP-Term in PSB1C3 by streaking in aerobic or anaerobic conditions)
• 15:49, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/29 ‎ (→3 - Digestion of BBa_K1155000, BBa_K1155003, BBa_K1155007 by Xbal/PstI)
• 15:48, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/28 ‎ (→2 - Transformation of FNR, RBS-FNR and RBS-BphR2 in DH5α)
• 15:48, 4 October 2013 (diff | hist) Team:Paris Saclay/Notebook/August/14 ‎ (→1 - Gibson assembly)

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