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BioBrick Restriction Digest

Supplies:

dsH2O

10x buffer (1,2,3,4)

100x BSA

Restriction Enzymes (EcoRI, Xbal, SpeI, PstI)

DNA

Steps:

1. Make a master mix with everything except the DNA. Keep everything on ice.

• depending on DNA concentration*

2. Pipet aliquots to each sample tube and add DNA.

3. Incubate at 37°C for 2-3h or let digest over night at room temperature.