Team:CU-Boulder/Notebook/Protocols

From 2013.igem.org

Embedding PDF Files: In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.

Embedding Google Docs: Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.

Chemically Competent Cells: This protocol details how to prepare chemically competent cells.

Related Experiments

Chemical Transformations: In this protocol we cover how to use our chemically competent cells to perform transformations.

Related Experiments

Plasmid Minipreps: Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.

Related Experiments

Experiment 3.1 - Promega Miniprep Protocol

Restriction (Double) Digests: Our method of performing restriction double digests is detailed in this protocol.

Gel Purification of DNA: Here we detail a fast and simple method of purifying DNA from a gel.

Ligation Reactions: This protocol covers how we set up out ligation reactions.

Homemade Buffer Compositions: The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.

Related Experiments

Recycling DNA Mini-columns: This protocol covers how to recycle mini-columns used in plasmid miniprep systems.

Related Experiments

Protein Miniprep: This protocol covers how to extract proteins from cells.

Protein Purification Via Agarose Gel: This protocol covers how to extract proteins from cells and purify it.

Related Experiments

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