Team:Paris Saclay/Notebook/July/5

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Notebook : July 5

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155000

1 - Electrophoresis of the digestion of BBa_K1155000 by NotI, HpaI, MluI

Sheng, Zhou

PCRPS050813.jpg
  • Well 1, 2 : 5µL of BBa_K1155000 digested by Not I+1µL of 6X loading dye
  • Well 3, 4 : 5µL of BBa_K1155000 digested by Hpa I+1µL of 6X loading dye
  • Well 5 : 6µL of DNA Ladder
  • Well 6, 7 : 5µL of BBa_K1155000 digested by Mlu I+1µL of 6X loading dye
  • Gel 0.8%

Expected sizes :

  • BBa_K1155000 : 111bp

We didn't obtain fragments at the right size. We will do digestions again.

2 - Extraction of BBa_K1155000 from DH5α

Abdou

Transformation of 07/04 works. We will extract DNA plasmid.

Protocol : High copy plamid extraction

Nanodrop :

  • BBa_K1155000 : 761ng/µl

By using NanoDrop, we found some errors which could be due to device malfunctional issue.

Objective : obtaining BBa_K1155003, BBa_K1155007

1 - Extraction of BBa_K592009 and BBa_I732017 from DH5α

Abdou

Transformation of 07/04 works. We will extract DNA plasmid.

Protocol : High copy plamid extraction

Nanodrop :

  • BBa_K592009 : 229ng/µl

By using NanoDrop, we found some errors which could be due to device malfunctional issue.


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