Team:TU-Delft/Protocol 3



Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.

Making glycerol stocks


  1. bacterial culture
  2. 80% glycerol


  1. The bacterial cells are grown overnight.
  2. In a glass tube take 250 μL of 80 % Glycerol solution.
  3. Pipet 1mL of the cell culture into 0.25 mL 80% glycerol and mix by vortexing and save in -80 °C freezer.

The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.