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Team:Evry/Notebook/w12
From 2013.igem.org
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<h1>Week 12: 2nd September - 8th September</h1> | <h1>Week 12: 2nd September - 8th September</h1> | ||
| + | <h2>TECAN</h2> | ||
| - | <h2> Plasmid 3</h2> | + | <h1>02/09/13</h1> |
| + | <h1>03/09/13</h1> | ||
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| + | <h2>Plasmid 3</h2> | ||
We launch another PC to get Enterobactins A, B, C, D, E and F genes. | We launch another PC to get Enterobactins A, B, C, D, E and F genes. | ||
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For the negative controle, we added 1 µL of distilled water in tube 8 and 39 µL of master mix 1.<br/> | For the negative controle, we added 1 µL of distilled water in tube 8 and 39 µL of master mix 1.<br/> | ||
For other tubes (6 genes and positive controle), we added 8 µL of genomic DNA in the master mix 1. Then add 40 µL of master mix 1' in each tube.<br/> | For other tubes (6 genes and positive controle), we added 8 µL of genomic DNA in the master mix 1. Then add 40 µL of master mix 1' in each tube.<br/> | ||
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Revision as of 15:30, 3 September 2013
Week 12: 2nd September - 8th September
TECAN
02/09/13
03/09/13
Plasmid 3
We launch another PC to get Enterobactins A, B, C, D, E and F genes. Add the recquired primers in each tube:- EntA: primers 065 and 066
- EntB: primers 067 and 068
- EntC: primers 069 and 070
- EntD: primers 071 and 072
- EntE: primers 073 and 074
- EntF: primers 075 and 076
- Positive controle: primers 009 and 010
- Negative controle: primers 009 and 010
For more details about our primers, see the corresponding page.
We then prepared a master mix 1 with:
- 9 x 27,5 = 247,5 µL of distilled water
- 9 x 1 = 9 µL of dNTPs
- 9 x 10 = 90 µL of Q
5 Buffer - 9 x 0,5 = 4,5 µL of Q
5 For the negative controle, we added 1 µL of distilled water in tube 8 and 39 µL of master mix 1.
For other tubes (6 genes and positive controle), we added 8 µL of genomic DNA in the master mix 1. Then add 40 µL of master mix 1' in each tube.
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