Team:TU-Munich/Results/Recombinant

From 2013.igem.org

(Difference between revisions)
(Investigated Proteins)
Line 18: Line 18:
|'''Affinity tag'''
|'''Affinity tag'''
|'''Size [kDa]'''
|'''Size [kDa]'''
 +
|'''Disulphid bridges'''
 +
|
 +
|-
 +
|Eryhtromycin Esterase
 +
|<partinfo>BBa_E0040</partinfo>
 +
|RFC25
 +
|c-term. ''Strep''tag II
 +
|
 +
|
|
|
|-
|-
|Laccase
|Laccase
|<partinfo>BBa_E0040</partinfo>
|<partinfo>BBa_E0040</partinfo>
-
|25
+
|RFC25
 +
|c-term. ''Strep''tag II
 +
|
 +
|
 +
|
 +
|-
 +
|Nano Luciferase
 +
|<partinfo>BBa_E0040</partinfo>
 +
|RFC25
|c-term. ''Strep''tag II
|c-term. ''Strep''tag II
|
|

Revision as of 10:30, 14 September 2013


Investigated Proteins

Text
Table 1:

Investigated Proteins

Protein BioBrick RFC Affinity tag Size [kDa] Disulphid bridges
Eryhtromycin Esterase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Laccase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Nano Luciferase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II



Economic Comparison of Remediation Scenarios

A comparison of biological and non-biological remediation has been published for all hazardous wast sites within the United States in which bioremediation with US$ 75 billion showed a clear advantages compared to conventional techniques with $ 750 billion http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997.

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997 Pimentel, D., Wilson, C., McCullum, C., Huang, R., Dwen, P., Flack, J. Tran, Q., Saltman, T., Cliff, T. (1997). Economic and environmental benefits of biodiversity. BioScience, Vol. 47, No. 11., pp. 747-757.