Team:TU-Munich/Results/Recombinant

From 2013.igem.org

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==Nano Luciferase==
==Nano Luciferase==
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[[File:TUM13 Animation_XylE.tif|thumb|right|320px| Figure 3:]]
[...] description
[...] description
[...] reaction
[...] reaction
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==XylE==
==XylE==
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[[File:TUM13 Animation_XylE.tif|thumb|right|320px| Figure 3:]]
[...] description
[...] description
[...] reaction
[...] reaction
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==Eryhtromycin Esterase==
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[[File:TUM13 Animation_EreB.tif|thumb|right|320px| Figure 3:]]
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[...] description
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[...] reaction
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[...] production
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===Analytical präparation===
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[...] Characterization
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<br><br>
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Revision as of 16:29, 14 September 2013


Text

Characterization of recombinant effector proteins

Text. See [2013.igem.org/Team:TU-Munich/Notebook/Methods proteinbiochemical methods] for further informatons.

Table 1:

Investigated Proteins

Protein BioBrick RFC Affinity tag Size [kDa] Disulphid bridges comment
Eryhtromycin Esterase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Laccase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
Nano Luciferase <partinfo>BBa_E0040</partinfo> RFC25 c-term. Streptag II
XylE


Eryhtromycin Esterase

File:TUM13 Animation EreB.tif
Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Laccase

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization

Nano Luciferase

File:TUM13 Animation XylE.tif
Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization



XylE

File:TUM13 Animation XylE.tif
Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization




Eryhtromycin Esterase

File:TUM13 Animation EreB.tif
Figure 3:

[...] description [...] reaction [...] production

Analytical präparation

[...] Characterization



References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
  2. http://udel.edu/~gshriver/pdf/Pimenteletal1997.pdf Pmentel et al., 1997 Pimentel, D., Wilson, C., McCullum, C., Huang, R., Dwen, P., Flack, J. Tran, Q., Saltman, T., Cliff, T. (1997). Economic and environmental benefits of biodiversity. BioScience, Vol. 47, No. 11., pp. 747-757.



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