"
Team:Calgary/Sandbox/Notebook/Protocols/RehydrationOfRegistryDNA
From 2013.igem.org
(Difference between revisions)
(Created page with "{{Team:Calgary/Main}} <html> <section id="Content"> <h1>Rehydration of Registry DNA</h1> <h2>Protocol</h2> <ul> <li>Biobrick parts are shipped from the registry in a dehydrated ...") |
|||
| Line 5: | Line 5: | ||
<h2>Protocol</h2> | <h2>Protocol</h2> | ||
| - | < | + | <ol> |
<li>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.</li> | <li>Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.</li> | ||
<li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;</li> | <li>Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;</li> | ||
| Line 11: | Line 11: | ||
<li>Let the water incubate at room temperature for 10 minutes;</li> | <li>Let the water incubate at room temperature for 10 minutes;</li> | ||
<li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li> | <li>Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.</li> | ||
| - | </ | + | </ol> |
</section> | </section> | ||
</html> | </html> | ||
Revision as of 04:51, 16 September 2013
Rehydration of Registry DNA
Protocol
- Biobrick parts are shipped from the registry in a dehydrated form. As such they must be rehydrated before they can be used.
- Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want;
- Add 10 μL of ddH2O (deionized water);
- Let the water incubate at room temperature for 10 minutes;
- Take 1 μL DNA and transform into your desired competent cells, plate out onto a plate with the correct antibiotic and grow overnight. Your goal here is to obtain single colonies.
