Team:TU-Munich/Notebook/Methods

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==Physcomitrella Methods==
==Physcomitrella Methods==
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The techniques used for the cultivation and manipulation of ''Physcomitrella patens'' are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible at [http://www.plant-biotech.net Plant-Biotech.net]
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The techniques used for the cultivation and manipulation of ''Physcomitrella patens'' are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible at [http://www.plant-biotech.net Plant-Biotech.net].
===Preparation of Knob-Media===
===Preparation of Knob-Media===

Revision as of 00:04, 16 June 2013


Molecular-biological Methods

Preparation of Nucleotide fragments

Digestion, Dephosphorylation

Hybridization of Oligonucleotides

Polymerase Chain Reaction

Site-directed Mutagenesis

Agarose Gel Electrophoresis

Ligation of DNA-Fragments

Microbiological Methods

Preparation of LB-Media

LB-Media

Reagent Amount

Transformation of E. coli

Cultivation of E. coli

Protein-biochemical Methods

Physcomitrella Methods

The techniques used for the cultivation and manipulation of Physcomitrella patens are based on the knowledge of the chair for Plantbiotechnology of Prof. Dr. Reski at Freiburg University which are availible at [http://www.plant-biotech.net Plant-Biotech.net].

Preparation of Knob-Media

Knob-Media

Reagent Amount

Cultivation of P. patens

Homogenization

Bioinformatical Methods

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