Team:Calgary/Sandbox/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples

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<h2>Protocol</h2>
<h2>Protocol</h2>
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<li>Culture and induce protein-producing bacteria</li>
<li>Centrifuge cells at max for 10 minutes. Discard supernatant</li>
<li>Centrifuge cells at max for 10 minutes. Discard supernatant</li>
<li>Resuspend cells in 2mL of lysis buffer</li>
<li>Resuspend cells in 2mL of lysis buffer</li>

Latest revision as of 23:31, 22 September 2013

Glass Beads Cell Lysis Protocol for Protein Samples

Reagents and Materials

  • 0.1m diameter glass beads
  • Lysis Buffer
  • Overnight induced cultures
  • 1.5mL tubes

Protocol

  1. Culture and induce protein-producing bacteria
  2. Centrifuge cells at max for 10 minutes. Discard supernatant
  3. Resuspend cells in 2mL of lysis buffer
  4. In a 1.5mL tube, add ~0.5mL of glass beads and 1mL of cells resuspended in lysis buffer
  5. Leave on ice for 5 min. Leave on BeadBeater or Vortex for 5 min. Repeat this cycle 6 times
  6. Centrifuge at max for 20 minutes
  7. Transfer supernatant (crude lysate) to a new tube and discard the glass beads