Team:CU-Boulder/Notebook/Protocols

From 2013.igem.org

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[[Protocols/GoogleDocEmbed Embedding Google Docs]]
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt>
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<dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd>
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</dl>
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</div>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a>  <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt>
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<dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd>
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</dl>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
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<dd>This protocol details how to prepare chemically competent cells.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_5_1">Experiment 5.1 - Calculating Competent Cell Efficiency</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_1">Experiment 6.1 - Competent Cell Efficiency</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_12_1">Experiment 12.1 - Calculating Competent Cell Efficiency</a></li>
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</ul>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt>
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<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a></li>
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</ul>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt>
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<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a></li>
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</ul>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt>
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<dd>Our method of performing restriction double digests is detailed in this protocol.</dd>
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</dl>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/DNAPurification">Gel Purification of DNA</a> <a href="https://static.igem.org/mediawiki/2013/1/19/Gel_Purification_of_DNA.pdf"><i class="icon-download"></i></a></dt>
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<dd>Here we detail a fast and simple method of purifying DNA from a gel.</dd>
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</dl>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/LigationReactions">Ligation Reactions</a> <a href="https://static.igem.org/mediawiki/2013/f/f2/Ligation_Reactions.pdf"><i class="icon-download"></i></a></dt>
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<dd>This protocol covers how we set up out ligation reactions.</dd>
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</dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt>
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<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_10_1">Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_2">Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers</a></li>
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</ul>
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</div>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt>
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<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_1">Experiment 11.1 - Mini-column Recycling Protocol</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_3">Experiment 11.3 - Mini-column Recycling Protocol Pt. II</a></li>
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<dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinMiniprep">Protein Miniprep</a> <a href="https://static.igem.org/mediawiki/2013/a/a7/Protein_Miniprep.pdf"><i class="icon-download"></i></a></dt>
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<dd>This protocol covers how to extract proteins from cells.</dd>
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</dl>
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<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinPurificationAgaroseGel">Protein Purification Via Agarose Gel</a> <a href="https://static.igem.org/mediawiki/2013/6/61/ProteinPurificationAgaroseGel.pdf"><i class="icon-download"></i></a></dt>
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<dd>This protocol covers how to extract proteins from cells and purify it.</dd>
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</dl>
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<h4>Related Experiments</h4>
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<ul>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_2">Experiment 6.2 – Gel Separation of Proteins Methods</a></li>
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<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 – Gel Separation of Proteins</a></li>
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</html>

Latest revision as of 00:57, 26 September 2013

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Embedding PDF Files
In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.
Embedding Google Docs
Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.
Chemically Competent Cells
This protocol details how to prepare chemically competent cells.

Related Experiments

Chemical Transformations
In this protocol we cover how to use our chemically competent cells to perform transformations.

Related Experiments

Plasmid Minipreps
Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.

Related Experiments

Restriction (Double) Digests
Our method of performing restriction double digests is detailed in this protocol.
Gel Purification of DNA
Here we detail a fast and simple method of purifying DNA from a gel.
Ligation Reactions
This protocol covers how we set up out ligation reactions.
Homemade Buffer Compositions
The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.

Related Experiments

Recycling DNA Mini-columns
This protocol covers how to recycle mini-columns used in plasmid miniprep systems.

Related Experiments

Protein Miniprep
This protocol covers how to extract proteins from cells.
Protein Purification Via Agarose Gel
This protocol covers how to extract proteins from cells and purify it.

Related Experiments

Retrieved from "http://2013.igem.org/Team:CU-Boulder/Notebook/Protocols"