Team:CU-Boulder/Notebook/Protocols

From 2013.igem.org

(Difference between revisions)

Latest revision as of 00:57, 26 September 2013

Embedding PDF Files: In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.

Embedding Google Docs: Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.

Chemically Competent Cells: This protocol details how to prepare chemically competent cells.

Related Experiments

Chemical Transformations: In this protocol we cover how to use our chemically competent cells to perform transformations.

Related Experiments

Plasmid Minipreps: Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.

Related Experiments

Experiment 3.1 - Promega Miniprep Protocol

Restriction (Double) Digests: Our method of performing restriction double digests is detailed in this protocol.

Gel Purification of DNA: Here we detail a fast and simple method of purifying DNA from a gel.

Ligation Reactions: This protocol covers how we set up out ligation reactions.

Homemade Buffer Compositions: The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.

Related Experiments

Recycling DNA Mini-columns: This protocol covers how to recycle mini-columns used in plasmid miniprep systems.

Related Experiments

Protein Miniprep: This protocol covers how to extract proteins from cells.

Protein Purification Via Agarose Gel: This protocol covers how to extract proteins from cells and purify it.

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 <div class="shadow-bottom">
 <dl>
-<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/EmbeddingPDF">Embedding PDF Files</a> <a href="https://static.igem.org/mediawiki/2013/9/99/Embedding_a_PDF_file_on_the_iGEM_Team_Websites.pdf"><i class="icon-download"></i></a></dt>
 <dd>In this protocol we cover how to embed a PDF on the iGEM team wiki pages. We also cover some of the additional CSS you will need in order to put this on your own site.</dd>
 </dl>
 </div>
 <div class="shadow-bottom">
 <dl>
-<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a></dt>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/GoogleDocEmbed">Embedding Google Docs</a>  <a href="https://static.igem.org/mediawiki/2013/0/04/Embedding_Google_Docs_on_the_Wiki_-_Google_Drive.pdf"><i class="icon-download"></i></a></dt>
 <dd>Here we cover how to embed a google document on the iGEM wiki. This is very useful during development since the document will continuously update and you can just convert it to a PDF at the end for a final version.</dd>
 </dl>
 </div>
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells">Chemically Competent Cells</a> <a href="https://static.igem.org/mediawiki/2013/d/da/Chemically_Competent_Cells.pdf"><i class="icon-download"></i></a></dt>
+<dd>This protocol details how to prepare chemically competent cells.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_1">Experiment 2.1 - Competent Cell Efficiency</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_5_1">Experiment 5.1 - Calculating Competent Cell Efficiency</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_1">Experiment 6.1 - Competent Cell Efficiency</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_12_1">Experiment 12.1 - Calculating Competent Cell Efficiency</a></li>
+</ul>
+</div>
-</html>
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations">Chemical Transformations</a> <a href="https://static.igem.org/mediawiki/2013/1/15/Chemical_Transformations.pdf"><i class="icon-download"></i></a></dt>
+<dd>In this protocol we cover how to use our chemically competent cells to perform transformations.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_1_1">Experiment 1.1 - Chemical Transformation Efficiency</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_2">Experiment 2.2 - Transformation Recovery Period</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_2_3">Experiment 2.3 - Transformation Recovery Medium</a></li>
+</ul>
+</div>
-<html><div class="shadow-bottom"></html>
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/PlasmidMinipreps">Plasmid Minipreps</a> <a href="https://static.igem.org/mediawiki/2013/0/08/Plasmid_Miniprep.pdf"><i class="icon-download"></i></a></dt>
+<dd>Here we detail the protocol for DNA plasmid minipreps for use with either the Qiagen QIAprep Spin Miniprep Kit or our homemade miniprep kit.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_3_1">Experiment 3.1 - Promega Miniprep Protocol</a></li>
+</ul>
+</div>
-[https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicallyCompetentCells Chemically Competent Cells]
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RestrictionDigests">Restriction (Double) Digests</a> <a href="https://static.igem.org/mediawiki/2013/f/f7/Restriction_Digests.pdf"><i class="icon-download"></i></a></dt>
+<dd>Our method of performing restriction double digests is detailed in this protocol.</dd>
+</dl>
+</div>
-[https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ChemicalTransformations Chemical Transformations]
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/DNAPurification">Gel Purification of DNA</a> <a href="https://static.igem.org/mediawiki/2013/1/19/Gel_Purification_of_DNA.pdf"><i class="icon-download"></i></a></dt>
+<dd>Here we detail a fast and simple method of purifying DNA from a gel.</dd>
+</dl>
+</div>
-[https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinMiniprep Protein Miniprep]
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/LigationReactions">Ligation Reactions</a> <a href="https://static.igem.org/mediawiki/2013/f/f2/Ligation_Reactions.pdf"><i class="icon-download"></i></a></dt>
+<dd>This protocol covers how we set up out ligation reactions.</dd>
+</dl>
+</div>
-[https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/DigestLysate Digest Lysate]
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/HomemadeBufferCompositions">Homemade Buffer Compositions</a> <a href="https://static.igem.org/mediawiki/2013/8/85/Homemade_Buffer_Compositions.pdf"><i class="icon-download"></i></a></dt>
+<dd>The composition of the buffers used in our homemade miniprep system, mini-column recycling protocol, and cell lysis for protein extraction are given in this protocol.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_10_1">Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_2">Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers</a></li>
+</ul>
+</div>
-[https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/Ligation Ligation]
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/RecyclingMinicolumns">Recycling DNA Mini-columns</a> <a href="https://static.igem.org/mediawiki/2013/d/dc/Recycling_DNA_Mini-columns.pdf"><i class="icon-download"></i></a></dt>
+<dd>This protocol covers how to recycle mini-columns used in plasmid miniprep systems.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_1">Experiment 11.1 - Mini-column Recycling Protocol</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_11_3">Experiment 11.3 - Mini-column Recycling Protocol Pt. II</a></li>
+</div>
-<html></div></html>
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinMiniprep">Protein Miniprep</a> <a href="https://static.igem.org/mediawiki/2013/a/a7/Protein_Miniprep.pdf"><i class="icon-download"></i></a></dt>
+<dd>This protocol covers how to extract proteins from cells.</dd>
+</dl>
+</div>
+<div class="shadow-bottom">
+<dl>
+<dt><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Protocols/ProteinPurificationAgaroseGel">Protein Purification Via Agarose Gel</a> <a href="https://static.igem.org/mediawiki/2013/6/61/ProteinPurificationAgaroseGel.pdf"><i class="icon-download"></i></a></dt>
+<dd>This protocol covers how to extract proteins from cells and purify it.</dd>
+</dl>
+<h4>Related Experiments</h4>
+<ul>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_6_2">Experiment 6.2 – Gel Separation of Proteins Methods</a></li>
+<li><a href="https://2013.igem.org/Team:CU-Boulder/Notebook/Experiments/Experiment_14_1">Experiment 14.1 – Gel Separation of Proteins</a></li>
+</div>
+</html>