Team:Macquarie Australia/Results

From 2013.igem.org

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<center><h7> <p>Promoter was ligated onto the genes and plated up on LB + CAM plates, we observed a lot of colony growth. We inoculated LB + CAM broth with a colony from the coresponding plate.</b></p></h7>
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<center><font size=4>Promoter Ligation</font size></center>
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&nbsp;&nbsp;&nbsp;&nbsp; Promoter was ligated onto the genes and plated up on LB + CAM plates, we observed a lot of colony growth.
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We inoculated LB + CAM broth with a colony from the coresponding plate. A variety of growth was
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observed, a few plates corresponding to a certain gense had a lot more colonies than others, this
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can be obesrved from the image with the description order of genes below. More colonies
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coresponding to a gene displayed more colony growth than others which can be obesrved from
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the image description showing the order of genes on the agar.
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Revision as of 03:12, 26 September 2013


Highlighted results, Images, Sequences, SDS, Every successful step we made towards our end goal

Results and Characterisation - [Under Construction]

Intro


Primary Results             Sequencing Results             Characterisation

Primary Results

[Description]


Sequencing Results

[Description]


BioBricksAmino Acid MatchMatch Percentage
POR317/31999%
ChlG320/320100%
ChlP421/42799%
ChlM278/28099%
YCF130/130100%
CTH1372/37499%
ChlI1353/353100%
DVR1299/30099%
Plastocyanin97/97100%
Gun4216/216100%

Characterisation



Promoter Ligation


     Promoter was ligated onto the genes and plated up on LB + CAM plates, we observed a lot of colony growth. We inoculated LB + CAM broth with a colony from the coresponding plate. A variety of growth was observed, a few plates corresponding to a certain gense had a lot more colonies than others, this can be obesrved from the image with the description order of genes below. More colonies coresponding to a gene displayed more colony growth than others which can be obesrved from the image description showing the order of genes on the agar.