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Team:Freiburg/Project/modeling
From 2013.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
<script language="JavaScript"> | <script language="JavaScript"> | ||
| - | function CrispR ( | + | function CrispR() { |
| + | alert("There are non nucleotide signs in your sequence. Plese check your sequence!") | ||
var TestVar = form.Sequenz.value; | var TestVar = form.Sequenz.value; | ||
var length = TestVar.length; | var length = TestVar.length; | ||
| Line 244: | Line 245: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | <input type="submit" value="submit" name="ok" onClick="CrispR( | + | <input type="submit" value="submit" name="ok" onClick="CrispR()" /> |
<input type="reset" value="reset"> | <input type="reset" value="reset"> | ||
</p> | </p> | ||
Revision as of 16:57, 26 September 2013
crRNA-Design
This tool helps you to design a crRNA-insert for pX334a. The oligos contain overhangs which fit to the BbsI-overhangs by pX334a.
For repression of gene transcription by targeting the coding sequence
it´s crucial to target the non template (= coding) DNA strand.
Therefore the oligos must be designed as
follows:
- Search at your desired target sequenz for a CCN (reverse complement of the PAM sequence) at the coding strand.
- Extract the following (3') 30 nucleotides.
- Extract the reverse complement.
- Add AAAC at the 5' end and GT at the 3' end. This will be your fist oligo.
- Take the sequence from step 2 and add TAAAAC at the 5' end. This will be your second oligo.
