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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
| + | {{Template:CU-BoulderHeader}} |
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| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;"> | + | <h1><center>The 2013 CU-Boulder iGEM Team</center></h1> |
| - | This is a template page. READ THESE INSTRUCTIONS.
| + | <center> |
| | + | <a href="https://static.igem.org/mediawiki/2013/9/9e/2013-CUiGEMTeam.jpg"> |
| | + | <img src="https://static.igem.org/mediawiki/2013/9/9e/2013-CUiGEMTeam.jpg" height="419" width="800"> |
| | + | </a> |
| | + | </center> |
| | + | <center> |
| | + | <p><b>From Left to Right:</b> Rebecca Zabinsky, Eli Lovelace, JF Lalonde, Robin Dowell, Raleigh Ems, Mike Brasino, Thomas Reynolds, Jo Hendrix, Jennifer Avena, Bill Heymann, Joe Rokicki, Alex Stemm-Wolf, Sarah Zimmerman, Phil Jensen</p> |
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| - | <!-- *** End of the alert box *** -->
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| - | {|align="justify"
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| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:CU-Boulder_logo.png|200px|right|frame]]
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| - | |[[Image:CU-Boulder_team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:CU-Boulder | Team CU-Boulder]]
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| - | |}
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| - | <!--- The Mission, Experiments --->
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:CU-Boulder|Home]]
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| - | !align="center"|[[Team:CU-Boulder/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=CU-Boulder Official Team Profile]
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| - | !align="center"|[[Team:CU-Boulder/Project|Project]]
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| - | !align="center"|[[Team:CU-Boulder/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:CU-Boulder/Modeling|Modeling]]
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| - | !align="center"|[[Team:CU-Boulder/Notebook|Notebook]]
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| - | !align="center"|[[Team:CU-Boulder/Safety|Safety]]
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| - | !align="center"|[[Team:CU-Boulder/Attributions|Attributions]]
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| - | |}
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| - | {{Caltech_iGEM_08|
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| - | Content=
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| - | __NOTOC__
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| - | ==Engineering multi-functional probiotic bacteria==
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| - | [[Image:Gut_flora_color.png|right|thumb|200px|Engineered gut flora]]
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| - | The human gut houses a diverse collection of microorganisms, with important implications for the health and welfare of the host. We aim to engineer a member of this microbial community to provide innovative medical treatments. Our work focuses on four main areas: (1) pathogen defense either by expression of [[Team:Caltech/Project/Phage_Pathogen_Defense|<font style="color:#BB4400">pathogen-specific bacteriophage</font>]] or by targeted bursts of [[Team:Caltech/Project/Oxidative_Burst|<font style="color:#BB4400">reactive oxygen species</font>]]; (2) prevention of birth defects by [[Team:Caltech/Project/Vitamins|<font style="color:#BB4400">folate over-expression</font>]] and delivery; (3) treatment of [[Team:Caltech/Project/Lactose_intolerance|<font style="color:#BB4400">lactose intolerance</font>]] by cleaving lactose to allow absorption in the large intestine; and (4) [[Team:Caltech/Project/Population_Variation|<font style="color:#BB4400">regulation</font>]] of these three treatment functions to produce renewable subpopulations specialized for each function. Our research demonstrates that synthetic biology techniques can be used to modify naturally occurring microbial communities for applications in biomedicine and biotechnology.
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| - | {{clear}}
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| - |
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| - | ==Why engineer gut microbes?==
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| - |
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| - | ===The large intestine: an ideal bioreactor===
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| - | [[Image:Digestive system diagram en.svg.png|thumb|200px|right|The human digestive tract]]
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| - | The human intestinal track is a perfect environment for bacteria. It is a 37°C mobile incubator with a constant stream of food. While bacteria are present in all parts of the intestinal track downstream of the stomach, the majority of those bacteria reside in the large intestine. There are approximately 10<sup>12</sup> bacterial per mL in the large intestinal lumen, composed of between 500-1000 different species of bacteria. Of these species, approximately 30 species comprise 99% of all bacteria in the large intestine. Estimating there is roughly 100 mL of feces in the large intestine, all the bacteria in our gut outnumber all the cells in the human body 100 to 1<sup>1</sup>.
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| - | {{clear}}
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| - |
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| - | ===Probiotic bacteria and other natural examples===
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| - | [[Image:Lacbr.jpg|thumb|left|Electron micrograph of ''Lactobacillus brevis'', a probiotic lactic acid bacterium]]
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| - | Most of the bacteria in our gut have yet to be characterized because they are difficult to culture, owing to their sensitivity to oxygen. However, several species are known. Many bacterial laboratory strains are derived from the well-known ''Escherichia coli'' (a non-pathogenic type), which is normally present in the large intestine. Because of its use in research, ''E. coli'' is the most well characterized bacteria to date. Another bacteria, ''Bacteroides fragilis'', plays an important role in proper development of the immune system and in controlling intestinal inflammation. Specifically, ''B. fragilis'' produces a starch called polysacharride A. In mice that had been raised in a sterile environment since birth (the intestinal track is initially sterile at birth and requires outside sources of bacteria to populate it), the immune system had less than normal levels of CD4+ killer T cells, a necessary white blood cell to battle infections. However, the CD4+ levels returned to normal when the mice were raised again in a sterile environment, except for the presence of ''B. fragilis''. Polysaccharide A alone, not the mere presence of Bacteroides fragilis, was responsible for the improvement, since mice raised with ''B. fragilis'' that could not produce polysaccharide A showed the same levels of CD4+ cell as the sterile mice<sup>2</sup>.
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| - | {{clear}}
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| - | Several bacterial species can cause disease in humans by infecting the gut. ''E. coli'' is commonly associated with food poisoning. ''Salmonela enterica'' is responsible for typhoid fever. ''Campylobacter jejuni'' and ''Shigella'' can cause bowl inflammation, diarrhea and dysentery. Cholera is caused by ''Vibrio cholerae'', which infected around 230,000 people and caused 6,300 deaths in 2006, according to the World Health Organization (WHO)[http://www.who.int/wer/2007/wer8231.pdf]. These pathogens typically cause illness in otherwise healthy people. There are other, more opportunistic bacteria, which infect people in hospital settings who are otherwise sick or undergoing treatment that puts them at greater risk for infection of their intestinal track. Paradoxically, the treatment of one pathogen with antibiotics can make that same patient more susceptible to infection of their gut by opportunistic pathogens. It is thought that the right balance of natural gut flora prevents these opportunistic pathogens from colonizing the colon<sup>3</sup>.
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| - | As a further example, humans cannot produce vitamin K, an important cofactor in blood clotting. Instead, the vitamin is provided by various species of the gut microbiota, which collectively produce more than 30 times the vitamin K recommended daily allowance<sup>4</sup>.
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| - |
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| - |
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| - | ===Nissle 1917: Probiotic, commercially available ''E. coli''===
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| - | [[Image:packshot_mutaflor.jpg|thumb|right|Mutaflor - a commercially available preparation of Nissle 1917]]
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| - | Nissle 1917 is a commercially available[http://www.ardeypharm.de/en/] non-pathogenic, probiotic strain of ''E. coli''. It has been successfully used to treat gastrointestinal disorders including colitis and intestinal bowel disease<sup>5</sup> and shows little immunostimulatory activity<sup>6</sup>. Engineered versions of the Nissle 1917 strain have been developed as anti-HIV<sup>7</sup> and anti-cholera<sup>8</sup> agents. The ability of Nissle 1917 to efficiently colonize the gut without provoking an inflammatory response makes it an ideal chassis for ''in situ'' applications in biomedicine and biotechnology.
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| - |
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| - | {{clear}}
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| - |
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| - | For more details, please see our [[Team:Caltech/Project|<font style="color:#BB4400">project</font>]] page.
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| - |
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| - | ===References===
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| - | # Hooper LV, Midtvedt T, and Gordon JI. '''How host-microbial interactions shape the nutrient environment of the mammalian intestine'''. ''Annu Rev Nutr'' 2002; 22 283-307.
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| - | # Mazmanian SK, Liu CH, Tzianabos AO, and Kasper DL. '''An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system'''. ''Cell'' 2005 Jul 15; 122(1) 107-18.
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| - | # Donskey CJ. '''The role of the intestinal tract as a reservoir and source for transmission of nosocomial pathogens'''. ''Clin Infect Dis'' 2004 Jul 15; 39(2) 219-26.
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| - | # Suttie JW. '''The importance of menaquinones in human nutrition'''. ''Annu Rev Nutr'' 1995; 15 399-417.
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| - | # Krammer HJ, Kamper H, von Bunau R, Zieseniss E, Stange C, Schlieger F, Clever I, and Schulze J. '''Probiotic drug therapy with E. coli strain Nissle 1917 (EcN): results of a prospective study of the records of 3,807 patients'''. ''Z Gastroenterol'' 2006 Aug; 44(8) 651-6.
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| - | # Westendorf AM, Gunzer F, Deppenmeier S, Tapadar D, Hunger JK, Schmidt MA, Buer J, and Bruder D. '''Intestinal immunity of Escherichia coli NISSLE 1917: a safe carrier for therapeutic molecules'''. ''FEMS Immunol Med Microbiol'' 2005 Mar 1; 43(3) 373-84.
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| - | # Rao S, Hu S, McHugh L, Lueders K, Henry K, Zhao Q, Fekete RA, Kar S, Adhya S, and Hamer DH. '''Toward a live microbial microbicide for HIV: commensal bacteria secreting an HIV fusion inhibitor peptide'''. ''Proc Natl Acad Sci U S A'' 2005 Aug 23; 102(34) 11993-8.
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| - | # Duan F and March JC. '''Interrupting Vibrio cholerae infection of human epithelial cells with engineered commensal bacterial signaling'''. ''Biotechnol Bioeng'' 2008 Sep 1; 101(1) 128-34.
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| - | }}
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| - | *********STOP! BEFORE YOU STEAL MY CODE, READ THIS!************
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| - | 2012 Colorado State iGEM Team Wiki Site
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| - | by Ethan Ho - Ethan.N.Ho@Gmail.com
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| - | -DC8800-Medium
| |
| - | -E9B300-Medium/Light
| |
| - | -925F00-Dark
| |
| - | -F1C900-Light
| |
| - | -BDAF77-Créme/white
| |
| - | -423600-Gold with grey overlay (27%)
| |
| - |
| |
| - | */
| |
| - |
| |
| - | /*********HIDE iGEM STUFF*********/
| |
| - |
| |
| - | #top-section, #footer-box, .firstHeading, .noprint, .printfooter, #catlinks, .visualclear, #contentSub, #siteSub {
| |
| - | z-index: 1;
| |
| - | position: absolute;
| |
| - | top: 0;
| |
| - | left: 0;
| |
| - | visibility: hidden;
| |
| - | width: 0;
| |
| - | height: 0;
| |
| - | border: none;
| |
| - | }
| |
| - |
| |
| - | /*********PAGE FORMATTING*********/
| |
| - |
| |
| - | #globalWrapper *, * {
| |
| - | border-spacing: 0;
| |
| - | border-collapse: collapse;
| |
| - | padding: 0;
| |
| - | margin: 0;
| |
| - | margin-left: 0;
| |
| - | margin-top: 0;
| |
| - | color: #CCC;
| |
| - | }
| |
| - |
| |
| - | body {
| |
| - | background: url(https://static.igem.org/mediawiki/2012/2/20/Beer1.png) top left fixed #DC8800;
| |
| - | background-size: 100% 100%;
| |
| - | height: 100%;
| |
| - | width: 100%;
| |
| - | }
| |
| - |
| |
| - | #content {
| |
| - | position: relative;
| |
| - | top: -10px;
| |
| - | margin-left: auto;
| |
| - | margin-right: auto;
| |
| - | margin-bottom: 100px;
| |
| - | padding: 0;
| |
| - | width: 800px;
| |
| - | height: auto;
| |
| - | border: none;
| |
| - | background: url(https://lh3.googleusercontent.com/-pusqszcwM8c/UAW5pfjsZgI/AAAAAAAAANs/0dLQIKngBhc/s488/trans1.png) repeat top left;
| |
| - |
| |
| - | /*font-family: 'HoeflerText-Regular', 'Hoefler Text', 'Times New Roman', 'serif';*/
| |
| - | font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;
| |
| - | }
| |
| - |
| |
| - | #footer {
| |
| - | position: absolute;
| |
| - | width: 100%;
| |
| - | bottom: 0;
| |
| - | z-index: 1;
| |
| - | }
| |
| - |
| |
| - | #content_main {
| |
| - | padding: 40px;
| |
| - | padding-top: 0;
| |
| - | }
| |
| - |
| |
| - | #side_bar {
| |
| - | position: fixed;
| |
| - | top: 150px;
| |
| - | right: 0;
| |
| - | width: 200px;
| |
| - | height: 250px;
| |
| - | z-index: 4;
| |
| - | cursor: pointer;
| |
| - | background: url(https://lh6.googleusercontent.com/-PEAQ4dNSF2Y/UAhs7D3J3VI/AAAAAAAAAOs/iRZZSBSWlK0/s300/tap1.png) no-repeat top right;
| |
| - | background-size: 100% 100%;
| |
| - | }
| |
| - |
| |
| - | #side_bar:hover {
| |
| - | background: url(https://lh6.googleusercontent.com/-6tCnE_Wfc1M/UAhs7N8xndI/AAAAAAAAAOs/AWmXapQj0nU/s300/tap2.png) no-repeat top left;
| |
| - | background-size: 100% 100%;
| |
| - | }
| |
| - |
| |
| - | /*********CONTENT_MAIN FORMATTING*********/
| |
| - |
| |
| - | #content_main a {
| |
| - | font-weight: bold;
| |
| - | color: #F1C900;
| |
| - | text-decoration: none;
| |
| - | }
| |
| - |
| |
| - | #content_main a:hover {
| |
| - | text-decoration: underline;
| |
| - | }
| |
| - |
| |
| - | #content_main h1, #content_main h2, #content_main h3, #content_main h4, #content_main h5 {
| |
| - | margin-bottom: 12px;
| |
| - | padding: 12px;
| |
| - | border: none;
| |
| - | border-bottom: solid 1px #CCC;
| |
| - | line-height: 120%;
| |
| - | }
| |
| - |
| |
| - | #content_main img {
| |
| - | border: 2px solid #CCC;
| |
| - | }
| |
| - |
| |
| - | #content_main ol, #content_main ul {
| |
| - | margin-left: 50px;
| |
| - | }
| |
| - |
| |
| - | #content_main ul {
| |
| - | list-style-image: url(https://lh4.googleusercontent.com/-mBAWcl14kCU/UAnEUwoTYmI/AAAAAAAAARs/Ow4zMrbuRNc/s6/bullet.png);
| |
| - | }
| |
| - |
| |
| - | #content_main td {
| |
| - | padding: 10px;
| |
| - | width: 50%;
| |
| - | }
| |
| - |
| |
| - | #content_main table, #content_main th, #content_main td {
| |
| - | background: none;
| |
| - | border: none;
| |
| - | }
| |
| - |
| |
| - | #content_main table * {
| |
| - | color: #CCC;
| |
| - | }
| |
| - |
| |
| - | /*********SLIDESHOW*********/
| |
| - |
| |
| - | #slideshow {
| |
| - | position: relative;
| |
| - | top: 0;
| |
| - | left: 0;
| |
| - | width: 100%;
| |
| - | height: 270px;
| |
| - | overflow: hidden;
| |
| - | }
| |
| - |
| |
| - | .slide {
| |
| - | position: absolute;
| |
| - | right: 0;
| |
| - | float: right;
| |
| - | width: auto;
| |
| - | height: 100%;
| |
| - | }
| |
| - |
| |
| - | /*********DROPDOWN CSS**********/
| |
| - |
| |
| - | #menu_bar td {
| |
| - | width: 33%;
| |
| - | }
| |
| - |
| |
| - | #menu_bar {
| |
| - | width: 100%;
| |
| - | overflow: hidden;
| |
| - | color: #CCC; /*******COLOR*******/
| |
| - | text-align: center;
| |
| - | background: none;
| |
| - | margin: 0;
| |
| - | }
| |
| - |
| |
| - | #menu_bar dt {
| |
| - | text-align: center;
| |
| - | width: 100%;
| |
| - | padding: 8px;
| |
| - | font-weight: bold;
| |
| - | cursor: pointer;
| |
| - | background: #925F00; /*******COLOR*******/
| |
| - | }
| |
| - |
| |
| - | #menu_bar dd {
| |
| - | position: absolute;
| |
| - | overflow: hidden;
| |
| - | width: 267px;
| |
| - | display: none;
| |
| - | background: #E9B300; /*******COLOR*******/
| |
| - | z-index: 4;
| |
| - | opacity: 0;
| |
| - | }
| |
| - |
| |
| - | #menu_bar ul {
| |
| - | width: 263px;
| |
| - | border: 2px solid #925F00; /*******COLOR*******/
| |
| - | list-style: none;
| |
| - | border-top: none;
| |
| - | }
| |
| - |
| |
| - | #menu_bar li {
| |
| - | display: inline;
| |
| - | }
| |
| - |
| |
| - | #menu_bar a, #menu_bar a:active, #menu_bar a:visited {
| |
| - | display: block;
| |
| - | padding: 5px;
| |
| - | text-decoration: none;
| |
| - | width: 100%;
| |
| - | color: #925F00; /*******COLOR*******/
| |
| - | border-top: 1px solid #925F00; /*******COLOR*******/
| |
| - | }
| |
| - |
| |
| - | #menu_bar a:hover {
| |
| - | background: #F1C900; /*******COLOR*******/
| |
| - | }
| |
| - |
| |
| - | .leftBorder {
| |
| - | border-left: solid 2px #CCC;
| |
| - | }
| |
| - |
| |
| - | </style>
| |
| - | <script type="text/javascript">
| |
| - | //newOpac: sets new opacity
| |
| - |
| |
| - | //*******************************************************************
| |
| - | //************************SLIDESHOW SCRIPT***************************
| |
| - | //*******************************************************************
| |
| - |
| |
| - | //Performs fade animation; main function
| |
| - | function fade(curElemID,isFading) {
| |
| - |
| |
| - | //length that each slide shows before fade (in milliseconds)
| |
| - | var slideStayTime = 3000;
| |
| - |
| |
| - | //length of fade (milliseconds)
| |
| - | var fadeTime = 800;
| |
| - |
| |
| - | //number of fade loops: > means smoother, more CPU-intensive transition
| |
| - | var numFadeLoops = 8;
| |
| - |
| |
| - | //In order for fade to work correctly, opacity must be rounded to
| |
| - | //the appropriate number of decimal places. roundTo defines this.
| |
| - | //For numFadeLoops < 20, roundTo = 1 will suffice. roundNum is the
| |
| - | //functional form.
| |
| - | var roundTo = 1;
| |
| - | var roundNum = Math.pow(10, roundTo);
| |
| - |
| |
| - | //Fetches the current slide element
| |
| - | var curElem = document.getElementById('slide' + curElemID);
| |
| - |
| |
| - | //Performs pre-fade functions. Function must be called with isFading = false.
| |
| - | if (isFading == false) {
| |
| - |
| |
| - | //Fetches the next slide element. If there is no next slide,
| |
| - | //return to slide1.
| |
| - | var nextElem = document.getElementById('slide' + (curElemID + 1));
| |
| - | if (!nextElem) {
| |
| - | var nextElem = document.getElementById('slide1');
| |
| - | } //end if
| |
| - |
| |
| - | //Brings current slide to front/fading z-index (3) and puts the
| |
| - | //next slide in the corresponding z-index (2). Slides that are
| |
| - | //neither the current or next slide should have z-index = 1.
| |
| - | curElem.style.zIndex = 3;
| |
| - | nextElem.style.zIndex = 2;
| |
| - |
| |
| - | //Sets a preliminary opacities. Optional
| |
| - | curElem.style.opacity = 1;
| |
| - | curElem.style.filter = 'alpha(opacity=100)';
| |
| - | nextElem.style.opacity = 1;
| |
| - | nextElem.style.filter = 'alpha(opacity=100)';
| |
| - |
| |
| - | } //end if
| |
| - |
| |
| - | //Fades out the current slide if it is not already completely faded yet
| |
| - | if (parseFloat(curElem.style.opacity) > 0) {
| |
| - |
| |
| - | //Sets new opacity and reruns the function
| |
| - | var newOpac = (Math.round(roundNum * (parseFloat(curElem.style.opacity) - (1/numFadeLoops)))) / roundNum;
| |
| - | curElem.style.opacity = newOpac;
| |
| - | curElem.style.filter = 'alpha(opacity=' + (newOpac * 100) + ')';
| |
| - | setTimeout(function(){fade(curElemID)}, (fadeTime/numFadeLoops));
| |
| - |
| |
| - | //Current slide has faded completely
| |
| - | } else {
| |
| - |
| |
| - | //Sets the current slide to a hidden state with full opacity
| |
| - | curElem.style.zIndex = 1;
| |
| - | curElem.style.opacity = 1;
| |
| - | curElem.style.filter = 'alpha(opacity=100)';
| |
| - |
| |
| - | //Sets next slide element. If there is no next slide,
| |
| - | //return to slide1.
| |
| - |
| |
| - | nextElemID = getNextSlide(curElemID);
| |
| - |
| |
| - | //Starts next fade. isFading = false is a required parameter
| |
| - | setTimeout(function(){fade(nextElemID, false)}, slideStayTime);
| |
| - |
| |
| - | //Menu bar functions
| |
| - | menuBar(curElemID);
| |
| - |
| |
| - | } //end if
| |
| - |
| |
| - | } //end function fade
| |
| - |
| |
| - | //Finds the next slide and returns its 'slide' + x id.
| |
| - | function getNextSlide(curElemID) {
| |
| - |
| |
| - | var nextElem = document.getElementById('slide' + (curElemID + 1));
| |
| - | if (nextElem) {
| |
| - | var nextElemID = curElemID + 1;
| |
| - | } else {
| |
| - | var nextElemID = 1;
| |
| - | } //end if
| |
| - |
| |
| - | return nextElemID;
| |
| - |
| |
| - | } //end function getNextSlide
| |
| - |
| |
| - | //Changes menu_bar element in coordination with slides
| |
| - | function menuBar(curElemID) {
| |
| - |
| |
| - | nextElemID = getNextSlide(curElemID);
| |
| - | var curMenuElem = document.getElementById('ddHeader' + curElemID);
| |
| - | var nextMenuElem = document.getElementById('ddHeader' + nextElemID);
| |
| - |
| |
| - | //menu_bar item change actions go here
| |
| - | nextMenuElem.style.background = '#DC8800'; /*******COLOR*******/
| |
| - |
| |
| - | //Change last menu_bar item back to its normal state
| |
| - | curMenuElem.style.background = '#925F00'; /*******COLOR*******/
| |
| - |
| |
| - | } //end function menuBar
| |
| - |
| |
| - | //*******************************************************************
| |
| - | //************************DROPDOWN SCRIPT****************************
| |
| - | //*******************************************************************
| |
| - |
| |
| - | var ddspeed = 10;
| |
| - | var ddtimer = 15;
| |
| - |
| |
| - | //Main function to handle the mouse events
| |
| - | function ddMain(id, direction) {
| |
| - |
| |
| - | var headerElem = document.getElementById('ddHeader' + id);
| |
| - | var contentElem = document.getElementById('ddContent' + id);
| |
| - | clearInterval(contentElem.timer);
| |
| - |
| |
| - | if (direction == 1) {
| |
| - | clearTimeout(headerElem.timer);
| |
| - | if (contentElem.maxh <= contentElem.offsetHeight) {
| |
| - | if (contentElem.maxh) {
| |
| - | return;
| |
| - | } //end if
| |
| - | } else if (!contentElem.maxh) {
| |
| - | contentElem.style.display = 'block';
| |
| - | contentElem.style.height = 'auto';
| |
| - | contentElem.maxh = contentElem.offsetHeight;
| |
| - | contentElem.style.height = '0px';
| |
| - | } //end if
| |
| - | contentElem.timer = setInterval(function(){ddSlide(contentElem,1)},ddtimer);
| |
| - | } else {
| |
| - | headerElem.timer = setTimeout(function(){ddCollapse(contentElem)},50);
| |
| - | } //end if
| |
| - |
| |
| - | } //end function ddMain
| |
| - |
| |
| - | //Collapse menu
| |
| - | function ddCollapse(contentElem) {
| |
| - |
| |
| - | contentElem.timer = setInterval(function(){ddSlide(contentElem, -1)},ddtimer);
| |
| - |
| |
| - | } //end function ddCollapse
| |
| - |
| |
| - | //Cancel the collapse if a user rolls over the dropdown
| |
| - | function cancelCollapse(id) {
| |
| - |
| |
| - | var headerElem = document.getElementById('ddHeader' + id);
| |
| - | var contentElem = document.getElementById('ddContent' + id);
| |
| - | clearTimeout(headerElem.timer);
| |
| - | clearInterval(contentElem.timer);
| |
| - |
| |
| - | if (contentElem.offsetHeight < contentElem.maxh) {
| |
| - | contentElem.timer = setInterval(function(){ddSlide(contentElem,1)}, ddtimer);
| |
| - | } //end if
| |
| - |
| |
| - | }
| |
| - |
| |
| - | //Incrementally expand/contract the dropdown and change the opacity
| |
| - | function ddSlide(contentElem,direction) {
| |
| - |
| |
| - | var curHeight = contentElem.offsetHeight;
| |
| - |
| |
| - | if (direction == 1) {
| |
| - | distance = Math.round((contentElem.maxh - curHeight) / ddspeed);
| |
| - | } else {
| |
| - | distance = Math.round(curHeight / ddspeed);
| |
| - | } //end if
| |
| - |
| |
| - | if (distance <= 1) {
| |
| - | if (direction == 1) {
| |
| - | distance = 1;
| |
| - | } //end if
| |
| - | } //end if
| |
| - |
| |
| - | contentElem.style.height = curHeight + (distance * direction) + 'px';
| |
| - | contentElem.style.opacity = curHeight / contentElem.maxh;
| |
| - | contentElem.style.filter = 'alpha(opacity=' + (curHeight * 100 / contentElem.maxh) + ')';
| |
| - |
| |
| - | if (curHeight < 2) {
| |
| - | if (direction != 1) {
| |
| - | clearInterval(contentElem.timer);
| |
| - | } //end if
| |
| - | } else if (curHeight > (contentElem.maxh - 2)) {
| |
| - | if (direction == 1) {
| |
| - | clearInterval(contentElem.timer);
| |
| - | } //end if
| |
| - | } //end if
| |
| - | } //end function ddSlide
| |
| - |
| |
| - | //*******************************************************************
| |
| - | //************************MISC FUNCTIONS*****************************
| |
| - | //*******************************************************************
| |
| - |
| |
| - | //Javascript link function
| |
| - | function linkTo(url) {
| |
| - |
| |
| - | window.location.href = url;
| |
| - |
| |
| - | } //end function linkTo
| |
| - |
| |
| - | //Simple function that performs startup scripts when prompted
| |
| - | function startFunctions () {
| |
| - |
| |
| - | setTimeout('fade(1,false)', 2000);
| |
| - | menuBar(0);
| |
| - |
| |
| - | } //end function startFunctions
| |
| - |
| |
| - | </script>
| |
| - | </head>
| |
| - | <body>
| |
| - | <img src="https://lh6.googleusercontent.com/-1Q-9hddFWuI/UCGVfn6W4wI/AAAAAAAAAVQ/e7PcCnXWO8o/s500/igem_home_logo.png" width="100" height="100" style="position: fixed; top: 10px; right: 10px; cursor: pointer;" onClick="linkTo('https://2012.igem.org/Main_Page')" />
| |
| - | <div style="border: solid red 1px;" id="menubar"></div>
| |
| - | <a id="side_bar" href="https://2012.igem.org/Team:Colorado_State/Safety"></a>
| |
| - | <div id="content_top">
| |
| - | <div id="slideshow">
| |
| - | <div style="position: absolute; top: 0; left: 0; height: 100%; width: 55%; z-index: 4; background: url(https://lh4.googleusercontent.com/-DDU8elUPsNM/UAm_sNRqnMI/AAAAAAAAARE/1Z2Xnvq4J1A/s600/fade1.png) top left; background-size: 100% 100%;">
| |
| - | <a href="https://2012.igem.org/Team:Colorado_State">
| |
| - | <div style="position: relative; top: 30px; left: 10px; border: solid red 1px; height: 220px; width: 287.65px; border: none; background: url(https://lh3.googleusercontent.com/-31sRsyZb5LU/UCKUYpC-gMI/AAAAAAAAAVk/MiL9X1XsNQ0/s640/logo2.png) no-repeat left; background-size: auto 100%;">
| |
| - | <img src="http://picasion.com/pic56/66eccba80910fafd431232af43e6e48f.gif" style="position: absolute; top: 12%; left: 28%; height: 22%; width: auto;" />
| |
| - | <img src="http://picasion.com/pic56/2f058d1a44eeb5749a3117dd586e79d6.gif" style="position: absolute; top: 26%; left: 21%; height: 12%; width: auto;" />
| |
| - | <img src="http://picasion.com/pic56/fc61f0752651a6be3920ad3917e72fa2.gif" style="position: absolute; top: 42%; left: 1.5%; height: 12%; width: auto;" />
| |
| - | </div>
| |
| - | </a>
| |
| - | </div>
| |
| - | <img id="slide1" class="slide" src="https://lh6.googleusercontent.com/-HUO1pRHwGn8/UA6_152AUUI/AAAAAAAAAUg/NIAPTu0rJeo/s912/slide2.jpg" style="z-index: 3;" />
| |
| - | <img id="slide2" class="slide" src="https://lh4.googleusercontent.com/-wv_cz741rxA/UA6_2SesX8I/AAAAAAAAAUo/kIBZr3Ydpi4/s912/slide1.jpg" />
| |
| - | <img id="slide3" class="slide" src="https://lh4.googleusercontent.com/-Atg7LS0jvu8/UA7Br49KLMI/AAAAAAAAAU8/LyqAernMIrM/s600/Brewery2-large.jpeg" />
| |
| - | </div>
| |
| - | <table id="menu_bar" cellspacing="0">
| |
| - | <tr>
| |
| - | <td>
| |
| - | <dl>
| |
| - | <dt id="ddHeader1" onClick="linkTo('https://2012.igem.org/Team:Colorado_State')">Home</dt>
| |
| - | <dd id="ddContent1">
| |
| - | <ul>
| |
| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Project">Home</a></li>
| |
| - | </ul>
| |
| - | </dd>
| |
| - | </dl>
| |
| - | </td>
| |
| - | <td>
| |
| - | <dl>
| |
| - | <dt id="ddHeader2" class="leftBorder" onMouseOver="ddMain(2,1)" onMouseOut="ddMain(2)">The Brew</dt>
| |
| - | <dd id="ddContent2" onMouseOver="cancelCollapse(2)" onMouseOut="ddMain(2)">
| |
| - | <ul>
| |
| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Project">Project Summary</a></li>
| |
| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Parts">Parts Submitted to the Registry</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Results">Results</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Safety">Safety</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Modeling">Modeling</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Notebook">Team Notebook</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Calendar">Calendar</a></li>
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| - | </ul>
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| - | <dt id="ddHeader3" class="leftBorder" onMouseOver="ddMain(3,1)" onMouseOut="ddMain(3)">The Brewers</dt>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Team">Meet the Students</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Instructors">Meet the Instructors</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Advisors">Meet the Advisors</a></li>
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| - | <li><a href="https://igem.org/Team.cgi?year=2012&team_name=Colorado_State">Official Team Page</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Sponsors">Sponsors</a></li>
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| - | <li><a href="https://2012.igem.org/Team:Colorado_State/Attributions">Attributions</a></li>
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| - | <h1>Our Gluten-Free Mission</h1>
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| - | <img src="https://lh6.googleusercontent.com/-cUic9qTY128/UA3HlblblkI/AAAAAAAAATI/m2Iu9XgpMFM/s512/gluten_free_keg.png" width="250" style="border: none; float: right; margin-left: 15px;" />
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| - | <p>If you have Celiac Disease or are Gluten-Intolerant (and are over 21), stop what you are doing right now. Your prayers for a great tasting gluten-free beer have finally been answered, or at least are in the process of being answered. Think of it as a delay in the mailroom. All of those nights when your friends were taunting you with the ice cold, delicious, golden liquid that gives courage to even the most faint of heart are quickly coming to an end. Your desire to enjoy a "cold one" without having a gut-wrenching reaction should be fullfilled by the end of the summer. The CSU iGEM team is working on producing a "gluten-free" beer that can be brewed from wheat or barley so you can savor that same great taste you know and love. So cross your fingers, bust out your lucky rabbits foot, and watch out for black cats because we are gearing up for something big, and it has never been done before.</p>
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| - | <h1>More Than a Great Beer</h1>
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| - | <img src="http://static.wix.com/media/5bd9eb_299a61aed7aee9a8fe1c2e1ca33ea264.jpg_srz_275_207_85_22_0.50_1.20_0.00_jpg_srz" width="250" style="float: left; margin-right: 15px;" />
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| - | <p>A gluten-free beer isn't the only thing we are working on. We are also designing primers for New Belgium Brewery, developing a novel gluten-detecting assay, and creating a computer-modeled simulation for the breakdown of gluten in beer. All of these various components have us teaming up with some of the best in the business, so don't forget to visit our sponsor and attribution pages.</p>
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| - | <h1>RamStrong</h1>
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| - | <img src="http://www.brandyleephotography.com/wp-content/uploads/Ram-Tough.jpg" width="250" style="float: right; margin-left: 15px;" />
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| - | <p>We proudly roam the steepest and most treacherous mountain sides, looking for adventure, and exploring whatever comes our way. We do not back down from challenges, instead we charge them head on. Although we do occasionally clash heads, we power through our disagreements, because... we are the CSU Rams!!!</p>
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| - | == '''Where we're from''' ==
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"
