Team:TU-Delft/Protocol 3

From 2013.igem.org

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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol2" style="text-decoration: none""><font color="#0080FF" size="3">Growing the Single Colonies from the Agar Plates</font></a> </li>
  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol2" style="text-decoration: none""><font color="#0080FF" size="3">Growing the Single Colonies from the Agar Plates</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_3" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_3" style="text-decoration: none""><font  
color="#0080FF" size="3"> Making glycerol stocks</font></a> </li>
color="#0080FF" size="3"> Making glycerol stocks</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4" style="text-decoration: none""><font  
color="#0080FF" size="3"> Miniprep Protocol</font></a> </li>
color="#0080FF" size="3"> Miniprep Protocol</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_5" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_5" style="text-decoration: none""><font  
color="#0080FF" size="3"> Restriction digestion</font></a> </li>
color="#0080FF" size="3"> Restriction digestion</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_6" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_6" style="text-decoration: none""><font  
color="#0080FF" size="3"> Ligation</font></a> </li>
color="#0080FF" size="3"> Ligation</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_7" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_7" style="text-decoration: none"" ><font  
color="#0080FF" size="3"> Gel Extraction Procedure</font></a> </li>
color="#0080FF" size="3"> Gel Extraction Procedure</font></a> </li>
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_8" style="text-decoration: none"" target="_blank"><font  
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_8" style="text-decoration: none""><font  
color="#0080FF" size="3">  PCR Purification</font></a> </li>
color="#0080FF" size="3">  PCR Purification</font></a> </li>
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<h2 align="center">Making glycerol stocks</h2>
<h2 align="center">Making glycerol stocks</h2>

Revision as of 13:40, 27 September 2013



Protocols

Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.


Making glycerol stocks

Requirements:

1. Glycerol 2. Pipettes 3. 1.5 mL Tubes 4. Culture Sample

Prodecure:

1. Label the tubes and add 180μL of glycerol.
2. Pipette out 1000 μL of the culture.
3. Add the culture to the tubes and store in -80°C

The glycerol stock can be used whenever required, by just adding 0.5 mL of stock into 5 mL of freshly prepared media.

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