Revision as of 15:20, 27 September 2013

Experiment > Parts Synthesis > 1st week

September 2nd
PCR following fragment.

Fragment	hbiF	Tc	lacI	FRT-Cm	FRT-Ap
Primer	pBAD-HbiF RTcN-hbiF	TcN TcC3	141-70 FRT-lacIC	R6Kori-Xho-3R R(FRT)2	lacI-R(FRT)2 mhpA-SaApR
Templete	hbiF (BBa_K880000)	Escherichia coli MG1655 recA⁻ (cell suspention)	Escherichia coli MG1655 genome	pSG76C	pSG76A2

@@ Line 20: / Line 20: @@
 <Table cellpadding="5">
 <Tr>
-<Td></Td><Td>PtetR1</Td><Td>PtetR2</Td><Td>FLP</Td><Td>Sm</Td>
+<Td>Fragment</Td><Td>PtetR1</Td><Td>PtetR2</Td><Td>FLP</Td><Td>Sm</Td><Td>araC-P</Td>
 </Tr>
 <Tr>
-<Td>Primer</Td><Td>CmNR-stu<Br>FLP-IRR/IRL</Td><Td>CmCR-stu<Br>FLP-IRR/IRL</Td><Td>FLP-L<Br>FLP-R</Td><Td>FLP-SaApL<Br>CmC</Td>
+<Td>Primer</Td><Td>CmNR-stu<Br>FLP-IRR/IRL</Td><Td>CmCR-stu<Br>FLP-IRR/IRL</Td><Td>FLP-L<Br>FLP-R</Td><Td>FLP-SaApL<Br>CmC</Td><Td>acaC-2<Br>BAD-C3</Td>
+</Tr>
+<Tr>
+<Td>Templete</Td><Td>IRR/IRL-GFP<Br>(BBa_K880002)</Td><Td>IRR/IRL-GFP<Br>(BBa_K880002)</Td><Td>pFTGT</Td><Td>m(C2-Ap)Sm</Td><Td>pBAD/Myc-HisA</Td>
+</Tr>
+</Table>
+</div>
+<div style="text-align:center;">
+<Table cellpadding="5">
+<Tr>
+<Td>Fragment</Td><Td>hbiF</Td><Td>Tc</Td><Td>lacI</Td><Td>FRT-Cm</Td><Td>FRT-Ap</Td>
+</Tr>
+<Tr>
+<Td>Primer</Td><Td>pBAD-HbiF<Br>RTcN-hbiF</Td><Td>TcN<Br>TcC3</Td><Td>141-70<Br>FRT-lacIC</Td><Td>R6Kori-Xho-3R<Br>R(FRT)2</Td><Td>lacI-R(FRT)2<Br>mhpA-SaApR</Td>
+</Tr>
+<Tr>
+<Td>Templete</Td><Td>hbiF<Br>(BBa_K880000)</Td><Td><i>Escherichia coli</i><Br>MG1655 recA⁻<Br>(cell suspention)</Td><Td><i>Escherichia coli</i><Br>MG1655 genome</Td><Td>pSG76C</Td><Td>pSG76A2</Td>
 </Tr>
 </Table>
 </div>
+<Br>
+<Br>
 <A href="https://2013.igem.org/Team:TMU-Tokyo/Notebook_experiment/2st">>>Go to next week</A>