Team:SJTU-BioX-Shanghai

From 2013.igem.org

(Difference between revisions)
Line 138: Line 138:
-
<p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> Redefinition of scaffold in Synthetic Biology by recruiting <i>E.coli’</i>s inner membrane as a natural two-dimensional scaffold. </p>
+
<p><strong><img src="/wiki/images/7/73/12SJTU_r.png"><font face="Cambria, serif " size="4"><font face="Wingdings " size="4"></font><font face="Cambria, serif " size="4"> The breakthrough we made:</font></strong> The quantitative regulation of gene expression in the level of genome. </p>
-
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The system we built:</font></strong> 6 membrane proteins orderly organized on the inner membrane of <i>E.coli</i>, the efficiency of which has been proved by fluorescence complementation assay and biosynthesis experiment.</p>
+
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The system we built:</font></strong> Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.</p>
-
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created – Membrane Accelerator:</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p>
+
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The device we created :</font></strong> A universal tool that serves to accelerate biochemical reactions in <i>E.coli</i>; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type <i>E.coli </i>and 9 fold compared to that with overexpressed cytoplasmic enzymes.</p>
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> device we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched. </p>
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> device we created – Membrane Rudder:</font></strong> A universal tool used to dynamically and artificially control biochemical reactions in <i>E.coli</i>; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched. </p>
-
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> New direction we proposed:</font></strong> The application of scaffold system in accelerating biodegradation pathway using our Membrane Accelerator.</p>
+
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> The experiment we did:</font></strong> having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.</p>
-
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> 42 well-characterized parts that could either be used directly or serve as a universal tool readily for potential scientific or engineering use.</p>
+
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Parts we submitted:</font></strong> .</p>
-
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> A club we established – BioCraft:</font></strong> The headquarter of our human practice programs, having come a long way in propagandizing Synthetic Biology and iGEM. Warmly-received activities have been held in and outside the campus. Several celebrities in different fields have shown support for us, laying a cornerstone for our future development.</p>
+
<p><strong><font face="Wingdings " size="4"><img src="/wiki/images/7/73/12SJTU_r.png"></font><font face="Cambria, serif " size="4"> Human practise:</font></strong> involvement in an official academic elite cultivation plan supported by national education ministry. Cooperation with corporation to try applying our project into industry. A survey associated with subject cross and a detailed relevant analysis of this survey.</p>
</div>
</div>
</div>
</div>

Revision as of 22:22, 27 September 2013

Abstract

How to regulate an entire metabolic pathway in vivo, delicately, accurately and conveniently, simultaneously controlling the expression of several genes? And how to optimize metabolic fluxes so as to maximize desired products? For decades, these questions remain haunting to “bioengineers”(especially synthetic biologists) who would like to have certain metabolites produced in live cells. The difficulty is again raised up when target genes endogenously reside on the genome, or have been implemented into the genome.

So this year, our team, SJTU-BioX-Shanghai, is dedicated to solve the problem. By integrating CRISPRi, the newly developed expression interference tool, with three light sensors (namely red light sensor, green light sensor and blue light sensor), we expect to provide a versatile platform on which researchers are able to quantitatively adjust the expression of any three enzymes in whatever pathway – to change the target, simply change the small guide RNA (sgRNA)!

In addition, with the luminous device and accompanying software, one could simply enter metabolomics data (catalytic rates of related enzymes) into our User Interface. The software will automatically conduct flux balance analysis (FBA), giving out suggestions for optimal expression value of different enzymes. Then, the expression value would be converted into intensity of lights that are finally to be exerted on cell cultures. It is -- just convenient! :)

Luminous System provide us a fascinating tool to adjust expression on genomic level.

Independent luminous sources work through a unbelievable project

Achievements

The breakthrough we made: The quantitative regulation of gene expression in the level of genome.

The system we built: Assure the effectiveness of the novel system CRISPRi , integration between CRISPRi system and several sensors.

The device we created : A universal tool that serves to accelerate biochemical reactions in E.coli; Rate of fatty acids synthesis was increased by 24 fold compared to wild-type E.coli and 9 fold compared to that with overexpressed cytoplasmic enzymes.

device we created – Membrane Rudder: A universal tool used to dynamically and artificially control biochemical reactions in E.coli; the direction of Violacein and Deoxyviolacein synthetic pathway was successfully switched.

The experiment we did: having a great summer filled with experiments, and planning to have fun at the Asian Jamboree!.

Parts we submitted: .

Human practise: involvement in an official academic elite cultivation plan supported by national education ministry. Cooperation with corporation to try applying our project into industry. A survey associated with subject cross and a detailed relevant analysis of this survey.

Sponsors


Retrieved from "http://2013.igem.org/Team:SJTU-BioX-Shanghai"