Team:Calgary/Notebook/Protocols/VerificationDigest

From 2013.igem.org

(Difference between revisions)
Line 22: Line 22:
<li>0.2µL enzyme 1</li>
<li>0.2µL enzyme 1</li>
<li>0.2µL enzyme 2</li>
<li>0.2µL enzyme 2</li>
-
<li>3.6µL ddH2O</li>
+
<li>3.6µL ddH<sub>2</sub>O (deionized water)</li>
</ul>
</ul>
<li>Incubate at 37 degrees Celsius for 1h</li>
<li>Incubate at 37 degrees Celsius for 1h</li>

Revision as of 23:16, 27 September 2013

Verification Digest

Reagents and Materials

  • Restriction enzymes
  • Appropriate buffer
  • Plasmid purified DNA
  • 1% agarose gel

Protocol

  1. In a tube, add (per reaction):
    • 4µL Plasmid purified DNA
    • 1µL appropriate buffer
    • 0.2µL enzyme 1
    • 0.2µL enzyme 2
    • 3.6µL ddH2O (deionized water)
  2. Incubate at 37 degrees Celsius for 1h
  3. Run 10µL in a 1% agarose gel at 100V

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