Team:British Columbia/Notebook/Protocols

From 2013.igem.org

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===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]===
===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]===
Restriction digest with BioBrick plasmid an inserts
Restriction digest with BioBrick plasmid an inserts
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===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]===
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Ligate Biobrick insert to vector
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]===
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]===

Revision as of 00:42, 28 September 2013

iGEM Home

Contents

Protocols

PCR

Instruction for colony PCR and regular PCR

Gel Verification

Make a gel and conduct gel elecrophoresis

BioBrick Restriction Digest

Restriction digest with BioBrick plasmid an inserts

BioBrick Ligation

Ligate Biobrick insert to vector

CRISPR Repeat-Spacer Array Assembly

Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Assembling Parts from Oligonucleotides

Assemble short parts from annealed oligonucleotides

(Multi) Site Directed Mutagenesis

Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.

Oligonucleotide Phosphorylation

Phosphorylate oligonucleotides prior to ligation

Electrocompetent Cell Production

Gas chromatography-mass spectrometry for biosynthetic product detection

GC-MS Protocol for assaying biosynthetic product production.

Make Combinatorial Part Libraries

Make combinatorial part libraries from compatible parts