Parts

Here is a summary of all the parts we submitted this year. Details of each part and characterization data can be found on the respective parts.

• We improved two TALE proteins from Slovenia 2012 iGEM team and submitted them in an inducible system (LacI) and with a his-6 tag such that they can be easily expressed and purified.
• We also designed two TALEs to bind to pathogenic E. coli genomic sequences.
• We submitted the E and K coils which can be used as connector for scaffolding proteins that cannot be fused together. We submitted these coils in the Friedburg assembly backbone such that other teams can easily create fusion proteins with them.
• We submitted two new reporters: Beta-Lactamase and Ferritin to the registry. We also submitted these with his-6 tag and in an inducible system (LacI) such that they are easy to express and isolate.
• We submitted our detector-reporter fusions: TALE-A with beta-lactamase, TALE-A with ferritin, TALE-B with beta lactamase, TALE-B with ferritin.

<groupparts>iGEM013 Calgary</groupparts>