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Team:Calgary/Notebook/Parts
From 2013.igem.org
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<p class="noIndent"> Here is a summary of all the parts we submitted this year. Details of each part and characterization data can be found on the respective parts.</p></html> | <p class="noIndent"> Here is a summary of all the parts we submitted this year. Details of each part and characterization data can be found on the respective parts.</p></html> | ||
| - | <ul><li>We improved two TALE proteins from Slovenia 2012 iGEM team and submitted them in an inducible system (LacI) and with a his-6 tag such that they can be easily expressed and purified. </li> | + | <ul><li>We improved two TALE proteins from Slovenia 2012 iGEM team and submitted them in an inducible system (LacI) and with a his-6 tag such that they can be easily expressed and purified. We also submitted binding sequences for these, both in separate plasmids, and on the same aplsmid. </li> |
<li>We also designed two of our own TALEs (TALE-A and TALE-B) to bind to pathogenic <i>E. coli </i> genomic sequences. </li> | <li>We also designed two of our own TALEs (TALE-A and TALE-B) to bind to pathogenic <i>E. coli </i> genomic sequences. </li> | ||
| - | <li>We submitted the E and K coils which can be used as | + | <li>We submitted the E and K coils which can be used as connectors for scaffolding proteins that cannot be fused together. We submitted these coils in the Friedburg assembly backbone such that other teams can easily create fusion proteins with them.</li> |
| - | <li> We submitted two new reporters: Beta-Lactamase and Ferritin to the registry. We also submitted these with | + | <li> We submitted two new reporters: Beta-Lactamase and Ferritin to the registry. We also submitted these with 6-his tags and in an inducible system (LacI) such that they are easy to express and isolate. </li> |
| - | <li> We submitted our fusions of: TALE-A with beta-lactamase | + | <li> We submitted our fusions of: TALE-A with beta-lactamase and TALE-A with ferritin in order to have detector-reporter constructs.</ul> |
<groupparts>iGEM013 Calgary</groupparts> | <groupparts>iGEM013 Calgary</groupparts> | ||
Revision as of 01:20, 28 September 2013
Parts
Parts
Here is a summary of all the parts we submitted this year. Details of each part and characterization data can be found on the respective parts.
- We improved two TALE proteins from Slovenia 2012 iGEM team and submitted them in an inducible system (LacI) and with a his-6 tag such that they can be easily expressed and purified. We also submitted binding sequences for these, both in separate plasmids, and on the same aplsmid.
- We also designed two of our own TALEs (TALE-A and TALE-B) to bind to pathogenic E. coli genomic sequences.
- We submitted the E and K coils which can be used as connectors for scaffolding proteins that cannot be fused together. We submitted these coils in the Friedburg assembly backbone such that other teams can easily create fusion proteins with them.
- We submitted two new reporters: Beta-Lactamase and Ferritin to the registry. We also submitted these with 6-his tags and in an inducible system (LacI) such that they are easy to express and isolate.
- We submitted our fusions of: TALE-A with beta-lactamase and TALE-A with ferritin in order to have detector-reporter constructs.
<groupparts>iGEM013 Calgary</groupparts>
