Team:OUC-China

From 2013.igem.org

(Difference between revisions)
 
(54 intermediate revisions not shown)
Line 3: Line 3:
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
-
<meta http-equiv="Content-Type" content="text/html; charset=gb2312" />
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<link href="http://oucast.com/igem/css/bootstrap.css" rel="stylesheet" media="screen">
+
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
-
<link href="http://oucast.com/igem/css/bootstrap-responsive.css" rel="stylesheet" media="screen">
+
<script src="http://code.jquery.com/jquery.min.js"></script>
-
 
+
-
 
+
<style>
<style>
 +
/*the igem Global setting*/
 +
* {
 +
  margin: 0;
 +
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
 +
body{
 +
  color: #34495e;
 +
}
 +
#bodyContent h1, #bodyContent h2{
 +
  margin-bottom:0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
  padding-top: 0;
 +
  padding-bottom: 0;
 +
  color: #34495e;
 +
}
 +
#globalWrapper {
 +
  font-size: 100%;
 +
}
#top-section{
#top-section{
   height:0px;
   height:0px;
Line 17: Line 38:
     background-color:transparent;
     background-color:transparent;
     overflow:hide;
     overflow:hide;
-
 
}
}
#p-logo{display:none;}
#p-logo{display:none;}
Line 32: Line 52:
#content
#content
{
{
-
border:none;
+
  border:none;
-
height: 100%;
+
  height: 100%;
-
margin:0;
+
  margin:0;
-
padding:0;
+
  padding:0;
-
width:100%;
+
  width:100%;
-
position: absolute;
+
  position: absolute;
-
background-color: #FFFFFF;
+
  background-color: #FFFFFF;
 +
  color: #34495e;
}
}
-
 
+
a:visited{
-
{
+
  color: #fff;
-
margin:0;
+
-
padding:0;
+
}
}
-
 
+
/*the nav settings
-
li {
+
----------------------------------------------- */
-
      font-weight:normal;
+
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
-
      font-size:12px;
+
  color: #1abc9c;
 +
  background-color: transparent;
}
}
-
 
+
.navbar-inverse .navbar-nav>li>a{
-
#menubar {
+
  color: #fff;
-
    background-color:transparent;
+
}
-
    position: relative;
+
.navbar-inverse .navbar-nav>li>a:hover{
-
    float:left;
+
  color: #1abc9c;
-
    white-space: nowrap;
+
  background-color:transparent;
-
    top:-6px;
+
}
-
    width: 490px;
+
.navbar-inverse .navbar-brand{
-
    z-index: 5000;
+
          color: #fff;
-
    font-family: sans-serif;
+
          padding: 0;
-
    font-size: 10%;
+
}
-
    line-height: 1em;
+
.navbar-inverse .navbar-brand img{
-
    z-index:99;
+
  height: 50px;
 +
  padding-left: 90px;
 +
}
 +
.navbar-inverse .navbar-brand p{
 +
  padding:12px 30px;
 +
  margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
  color: #1abc9c;
 +
}
 +
.navbar-inverse{
 +
  border-color: transparent;
 +
  background: #34495e;
 +
  font-weight: bold;
 +
  filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
 +
}
 +
.dropdown-menu{
 +
  background-color:#34495e;
 +
  border:none;
 +
  -webkit-box-shadow:none;
 +
  box-shadow: none;
 +
}
 +
.dropdown-menu>li>a{
 +
  color: #fff;
 +
  font-weight: bold;
 +
}
 +
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
 +
  color:#1abc9c;
 +
  background-color: transparent;
}
}
 +
/*add dropdown-submenu to btV3*/
 +
.dropdown-submenu{position:relative;}
 +
.dropdown-submenu>.dropdown-menu{top:0;left:100%;margin-top:-6px;margin-left:-1px;-webkit-border-radius:0 6px 6px 6px;-moz-border-radius:0 6px 6px 6px;border-radius:0 6px 6px 6px;}
 +
.dropdown-submenu:hover>.dropdown-menu{display:block;}
 +
.dropdown-submenu>a:after{display:block;content:" ";float:right;width:0;height:0;border-color:transparent;border-style:solid;border-width:5px 0 5px 5px;border-left-color:#cccccc;margin-top:5px;margin-right:-10px;}
 +
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
 +
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
 +
/*the unslider css*/
 +
.banner {
 +
  position: relative;
 +
  width: 100%;
 +
  overflow: auto;
 +
  font-size: 18px;
 +
  line-height: 24px;
 +
  text-align: center;
 +
  color: #fff;
 +
  background: ;
 +
  box-shadow: 0 1px 2px rgba(0,0,0,.25);
 +
}
 +
  .banner ul {
 +
    list-style: none;
 +
    width: 300%;
 +
    margin: 0;
 +
  }
 +
  .banner ul li {
 +
    display: block;
 +
    float: left;
 +
    width: 33%;
 +
    min-height: 350px;
 +
    -o-background-size: 100% 100%;
 +
    -ms-background-size: 100% 100%;
 +
    -moz-background-size: 100% 100%;
 +
    -webkit-background-size: 100% 100%;
 +
    background-size: 100% 100%;
-
/* GLOBAL STYLES
+
     box-shadow: inset 0 -3px 6px rgba(0,0,0,.1);
-
     -------------------------------------------------- */
+
  }
-
    /* Padding below the footer and lighter body text */
+
 +
  .banner .inner {
-
    h1 {
 
-
color:#0099FF;
 
-
}
 
-
    body {
 
-
      padding-bottom: 40px;
 
-
      color: #5a5a5a;
 
-
  background: #e5e5e5 url(image/moon.png);
 
-
  overflow-x: hidden;
 
-
  -webkit-user-select: none;
 
-
  -webkit-tap-highlight-color: rgba(0,0,0,0);
 
-
    }
 
-
     .jumbotron {
+
     padding: 160px 0 110px;
-
        margin: 60px 0;
+
  }
-
        text-align: center;
+
-
      }
+
-
      .jumbotron h1 {
+
-
        font-size: 72px;
+
-
        line-height: 1;
+
-
      }
+
-
      .jumbotron .btn {
+
-
        font-size: 21px;
+
-
        padding: 14px 24px;
+
-
      }
+
-
      /* Supporting marketing content */
+
  .banner h1, .banner h2 {
-
      .marketing {
+
    font-size: 40px;
-
        margin: 60px 0;
+
    line-height: 52px;
-
      }
+
    border-bottom:#fff;
-
      .marketing p + h4 {
+
    color: #fff;
-
        margin-top: 28px;
+
  }
-
      }
+
-
     /* CUSTOMIZE THE NAVBAR
+
  .banner .btn {
-
     -------------------------------------------------- */
+
     display: inline-block;
 +
     margin: 25px 0 0;
 +
    padding: 9px 22px 7px;
 +
    clear: both;
-
     /* Special class on .container surrounding .navbar, used for positioning it into place. */
+
     color: #fff;
-
     .navbar-wrapper {
+
     font-size: 12px;
-
      position: absolute;
+
    font-weight: bold;
-
      top: 0;
+
    text-transform: uppercase;
-
      left: 0;
+
    text-decoration: none;
-
      right: 0;
+
-
      z-index: 10;
+
-
      margin-top: 20px;
+
-
      margin-bottom: -90px; /* Negative margin to pull up carousel. 90px is roughly margins and height of navbar. */
+
-
    }
+
-
    .navbar-wrapper .navbar {
+
 +
    border: 2px solid rgba(255,255,255,.4);
 +
    border-radius: 5px;
 +
  }
 +
    .banner .btn:hover {
 +
      background: rgba(255,255,255,.05);
     }
     }
-
 
+
     .banner .btn:active {
-
    /* Remove border and change up box shadow for more contrast */
+
       -webkit-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
     .navbar .navbar-inner {
+
       -moz-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
       border: 0;
+
      -ms-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
       -webkit-box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
      -o-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
        -moz-box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
      filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
-
              box-shadow: 0 2px 20px rgba(10,0,0,.25);
+
     }
     }
-
    /* Downsize the brand/project name a bit */
+
  .banner .btn, .banner .dot {
-
    .navbar .brand {
+
    -webkit-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
-
      padding: 14px 20px 16px; /* Increase vertical padding to match navbar links */
+
    -moz-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
-
      font-size: 16px;
+
    -ms-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
-
      font-weight: bold;
+
    -o-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
-
      text-shadow: 0 -1px 0 rgba(0,0,0,.5);
+
     filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
-
     }
+
  }
-
     /* Navbar links: increase padding for taller navbar */
+
  .banner .dots {
-
     .navbar .nav > li > a {
+
     position: absolute;
-
       padding: 15px 20px;
+
    left: 0;
-
    }
+
    right: 0;
 +
    bottom: 20px;
 +
    margin: 0;
 +
  }
 +
     .banner .dots li {
 +
       display: inline-block;
 +
      width: 10px;
 +
      height: 10px;
 +
      margin: 0 4px;
-
    /* Offset the responsive button for proper vertical alignment */
+
       text-indent: -999em;
-
    .navbar .btn-navbar {
+
-
       margin-top: 10px;
+
-
    }
+
 +
      border: 2px solid #fff;
 +
      border-radius: 6px;
 +
      cursor: pointer;
 +
      opacity: .4;
-
    /* CUSTOMIZE THE CAROUSEL
+
      -webkit-transition: background .5s, opacity .5s;
-
    -------------------------------------------------- */
+
      -moz-transition: background .5s, opacity .5s;
-
 
+
       transition: background .5s, opacity .5s;
-
    /* Carousel base class */
+
-
    .carousel {
+
-
       margin-bottom: 60px;
+
     }
     }
 +
      .banner .dots li.active {
 +
        background: #fff;
 +
        opacity: 1;
 +
      }
-
     .carousel .container {
+
  .banner .arrows {
-
       position: relative;
+
    position: absolute;
-
       z-index: 9;
+
    bottom: 20px;
 +
    right: 20px;
 +
    color: #fff;
 +
  }
 +
     .banner .arrow {
 +
       display: inline;
 +
       padding-left: 10px;
 +
      cursor: pointer;
     }
     }
-
    .carousel-control {
+
/*features*/
-
      height: 80px;
+
.features {
-
      margin-top: 0;
+
  overflow: hidden;
-
      font-size: 120px;
+
  padding: 50px 0;
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
}
-
      background-color: transparent;
+
  .features li {
-
      border: 0;
+
    position: relative;
-
      z-index: 10;
+
    padding: 0 25px 0 40px;
-
    }
+
    list-style: none;
-
     .carousel .item {
+
     width: 25%;
-
      height: 400px;
+
     float: left;
-
     }
+
  }
-
     .carousel img {
+
     .features li:before {
 +
      content: '';
       position: absolute;
       position: absolute;
 +
      left: 0;
       top: 0;
       top: 0;
-
      left: 0;
 
-
      min-width: 100%;
 
-
      height: 400px;
 
-
    }
 
-
    .carousel-caption {
+
       display: block;
-
       background-color: transparent;
+
       width: 24px;
-
       position: static;
+
       height: 22px;
-
      max-width: 550px;
+
-
       padding: 0 20px;
+
-
      margin-top: 200px;
+
-
    }
+
-
    .carousel-caption h1,
+
-
    .carousel-caption .lead {
+
-
      margin: 0;
+
-
      line-height: 1.25;
+
-
      color: #fff;
+
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
    }
+
-
    .carousel-caption .btn {
+
-
      margin-top: 10px;
+
-
    }
+
-
 
+
      background: url('img/icons.png');
-
 
+
-
    /* MARKETING CONTENT
+
-
    -------------------------------------------------- */
+
-
 
+
-
    /* Center align the text within the three columns below the carousel */
+
-
    .marketing .span6 {
+
-
      text-align: center;
+
     }
     }
-
    .marketing h2 {
+
       .features li.browser:before { background-position: 0 -44px; }
-
       font-weight: normal;
+
       .features li.height:before { background-position: 0 -22px; }
-
      margin-left: 200px;
+
       .features li.responsive:before { background-position: 0 -66px; }
-
      margin-right: 60px;
+
  .features b {
-
    }
+
    display: block;
-
    .marketing .span6 p {
+
    padding-bottom: 6px;
-
      margin-left: 200px;
+
  }
-
      margin-right: 60px;
+
.wrap {
-
    }
+
  margin: 0 auto;
-
 
+
  max-width: 1170px;
-
 
+
-
    /* Featurettes
+
-
    ------------------------- */
+
-
 
+
-
    .featurette-divider {
+
-
      margin: 80px 0; /* Space out the Bootstrap <hr> more */
+
-
    }
+
-
    .featurette {
+
-
      padding-top: 120px; /* Vertically center images part 1: add padding above and below text. */
+
-
      overflow: hidden; /* Vertically center images part 2: clear their floats. */
+
-
    }
+
-
    .featurette-image {
+
-
       margin-top: -120px; /* Vertically center images part 3: negative margin up the image the same amount of the padding to center it. */
+
-
    }
+
-
 
+
-
    /* Give some space on the sides of the floated elements so text doesn't run right into it. */
+
-
    .featurette-image.pull-left {
+
-
      margin-right: 40px;
+
-
    }
+
-
    .featurette-image.pull-right {
+
-
      margin-left: 40px;
+
-
    }
+
-
 
+
-
    /* Thin out the marketing headings */
+
-
    .featurette-heading {
+
-
      font-size: 50px;
+
-
      font-weight: 300;
+
-
      line-height: 1;
+
-
      letter-spacing: -1px;
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    /* RESPONSIVE CSS
+
-
    -------------------------------------------------- */
+
-
 
+
-
    @media (max-width: 979px) {
+
-
 
+
-
       .container.navbar-wrapper {
+
-
        margin-bottom: 0;
+
-
        width: auto;
+
-
      }
+
-
      .navbar-inner {
+
-
        border-radius: 0;
+
-
        margin: -20px 0;
+
-
      }
+
-
 
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        width: auto;
+
-
        height: 400px;
+
-
      }
+
-
 
+
-
      .featurette {
+
-
        height: auto;
+
-
        padding: 0;
+
-
      }
+
-
      .featurette-image.pull-left,
+
-
      .featurette-image.pull-right {
+
-
        display: block;
+
-
        float: none;
+
-
        max-width: 40%;
+
-
        margin: 0 auto 20px;
+
-
      }
+
-
    }
+
-
 
+
-
 
+
-
    @media (max-width: 767px) {
+
-
 
+
-
      .navbar-inner {
+
-
        margin: -20px;
+
-
      }
+
-
 
+
-
      .carousel {
+
-
        margin-left: -20px;
+
-
        margin-right: -20px;
+
-
      }
+
-
      .carousel .container {
+
-
 
+
-
      }
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel-caption {
+
-
        width: 65%;
+
-
        padding: 0 70px;
+
-
        margin-top: 100px;
+
-
      }
+
-
      .carousel-caption h1 {
+
-
        font-size: 30px;
+
-
      }
+
-
      .carousel-caption .lead,
+
-
      .carousel-caption .btn {
+
-
        font-size: 18px;
+
-
      }
+
-
 
+
-
      .marketing .span4 + .span4 {
+
-
        margin-top: 40px;
+
-
      }
+
-
 
+
-
      .featurette-heading {
+
-
        font-size: 30px;
+
-
      }
+
-
      .featurette .lead {
+
-
        font-size: 18px;
+
-
        line-height: 1.5;
+
-
      }
+
-
 
+
-
    }
+
-
+
-
/* = 通用 Global
+
-
----------------------------------------------- */
+
-
a:not(.btn) {
+
-
color: #00699E;
+
-
-webkit-transition: 300ms;
+
-
-moz-transition: 300ms;
+
-
transition: 300ms;
+
}
}
-
a:hover:not(.btn),
+
/*footer*/
-
a:active:not(.btn) {
+
footer{
-
color: #9E0000;
+
  margin-top: 100px;
-
text-decoration: none;
+
  background-color: #eceff1;
-
-webkit-transform: translateY(-8px);
+
  color: #bdc1c5;
-
-moz-transform: translateY(-8px);
+
  font-size: 17px;
-
transform: translateY(-8px);
+
  padding: 0;
}
}
-
 
+
.footer-banner{
-
hr.soften {
+
  background-color: #34495e;
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                   <a tabindex="-1" href="#">RNA guardian</a>
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                       <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
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 +
          <div class="inner">
 +
            <h1>WE DID IT !</h1>
 +
            <p>Constructing an Artificial Organelle（Intracellular Compartment） in <i>E.coli</i></p>
 +
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn the story behind this picture</a>
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          <div class="inner">
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            <h1>Compression of Metabolic Pathways</h1>
 +
            <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
 +
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Design">learn more</a>
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            <h1>Microfluidic Chip Magnetism Detection</h1>
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              <p class="lead">Cras justo odio, dapibus ac facilisis in, egestas eget quam. Donec id elit non mi porta gravida at eget metus. Nullam id dolor id nibh ultricies vehicula ut id elit.</p>
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             <h1>Factors Considered in RNA guardian</h1>
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            <p><br /></p>
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      <h3><b>Features Of Our Project</b></h3><hr>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
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          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
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          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
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          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
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        <h1>Abstract</h1>
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        <p class="lead">Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.</p>
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        <p class="lead">1.We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.<br/>2.We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.<br/>3.We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.<br/>4.We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in High oxygen partial pressure environment.
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         <p class="lead" ><font color="#0099FF">Reconstructing the Magnetosome Membrane in E. coli</font></p>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
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          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
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        <h3><b>Introduction</b></h3><hr>
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        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
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Latest revision as of 03:38, 28 September 2013

    Features Of Our Project

  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

    Learn more
  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

    Learn more
  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

    Learn more
  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

    Learn more

Introduction

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

Learn more

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