Team:OUC-China

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<body>
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    <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
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      <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
 +
    </a>
 +
    <a class="navbar-brand" href="https://2013.igem.org/Team:OUC-China">
 +
      <p>OUC-China IGEM 2013</p>
 +
    </a>
 +
  </div>
 +
    <div class="collapse navbar-collapse navbar-ex1-collapse">
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      <ul class="nav navbar-nav pull-right">
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      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
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            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
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              <ul class="dropdown-menu">
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          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Overview">Overview</a></li>
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Achievement">Achievement & judge criteria</a></li>
 +
                    <li class="dropdown-submenu">
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                      <a tabindex="-1" href="#">Intracellular compartment</a>
 +
                <ul class="dropdown-menu">
 +
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Review">Review</a></li>
 +
                        <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Design">Design</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Microfluidics</a></li>
 +
              <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Results">Results</a></li>
 +
                </ul>
 +
            </li>
 +
                  <li class="dropdown-submenu">
 +
                  <a tabindex="-1" href="#">RNA guardian</a>
 +
          <ul class="dropdown-menu">
 +
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Introduction">Introduction</a></li>
 +
                      <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Design">Design</a></li>
 +
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/RNA guardian/Results">Result</a></li>
 +
            <li><a tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Reference">Reference</a></li>
 +
            </ul>
 +
          </li>
 +
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Part description">Part description</a></li>
 +
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Future work">Future work</a></li>
 +
                </ul>
 +
            </li>
 +
            <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Modeling<b class="caret"></b></a>
 +
                <ul class="dropdown-menu">
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Magnetic Analysis">Magnetic Analysis</a></li>
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Model in RNA guardian">Model in RNA guardian</a></li>
 +
                </ul>
 +
            </li>
 +
            <li><a  href="https://2013.igem.org/Team:OUC-China/Human Practice">Human Practice</a></li>
 +
      <li class="dropdown">
 +
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<b class="caret"></b></a>
 +
              <ul class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Members">Members</a></li>
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Instructors">Instructors</a></li>
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Advisor">Advisor</a></li>
 +
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab">Lab</a></li>
 +
          <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Acknowledgement">Acknowledgement</a></li>
 +
        </ul>
 +
            </li>
 +
      <li class="dropdown"><a  href="https://2013.igem.org/Team:OUC-China/Safety">Safety</a></li>
 +
      <li class="dropdown">
 +
                <a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<b class="caret"></b></a>
 +
                <ul id="menu2" class="dropdown-menu" role="menu" aria-labelledby="dropdownMenu">
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Lab note">Lab note</a></li>
 +
                  <li role="presentation"><a role="menuitem" tabindex="-1" href="https://2013.igem.org/Team:OUC-China/Protocol">Protocol</a></li>
 +
                </ul>
 +
            </li>
 +
    </ul>
 +
  </div>
 +
</div>   
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 +
<div class="banner">
 +
      <ul>
 +
        <li style="background-image: url('https://static.igem.org/mediawiki/igem.org/9/9e/Tital.jpg');">
 +
          <div class="inner">
 +
            <h1>WE DID IT !</h1>
 +
            <p>Constructing an Artificial Organelle（Intracellular Compartment） in <i>E.coli</i></p>
 +
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Results">learn the story behind this picture</a>
 +
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 +
            <h1>Compression of Metabolic Pathways</h1>
 +
            <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
 +
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Design">learn more</a>
 +
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+
            <h1>Microfluidic Chip Magnetism Detection</h1>
-
// rAF
+
            <p><br></p>
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            <a class="btn" href="https://2013.igem.org/Team:OUC-China/Microfluidics" style="color:#1abc9c;">learn more</a>
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        </li>
-
window.mozRequestAnimationFrame ||
+
        <li style="background-image: url('https://static.igem.org/mediawiki/2013/7/75/Ouc-slide4.jpg');">
-
window.msRequestAnimationFrame ||
+
          <div class="inner">
-
window.oRequestAnimationFrame ||
+
            <h1>Factors Considered in RNA guardian</h1>
-
function(f) {
+
            <p><br /></p>
-
window.setTimeout(f,1e3/60);
+
            <a class="btn" href="https://2013.igem.org/Team:OUC-China/RNA_guardian/Design">Learn more</a>
-
}
+
          </div>
-
}();
+
        </li>
-
+
      </ul>
-
var canvas = document.querySelector('canvas');
+
  </div>
-
var ctx = canvas.getContext('2d');
+
  <!--the slidershow end-->
 +
  <div class="features">
 +
      <ul class="wrap">
 +
      <h3><b>Features Of Our Project</b></h3><hr>
 +
        <li>
 +
          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
 +
          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Results">Learn more</a>
 +
        </li>
 +
        <li>
 +
          <b><span class="glyphicon glyphicon-ok-sign"></span> RNA Guardian</b>
 +
          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Instruction">Learn more</a>
 +
        </li>
 +
        <li>
 +
          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
 +
          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
 +
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Microfluidics">Learn more</a>
-
var W = canvas.width = window.innerWidth;
+
        </li>
-
var H = canvas.height = window.innerHeight;
+
        <li>
-
 
+
          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
-
// Our SpaceShip Constructor
+
          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
-
var SpaceShip = function() {
+
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Design">Learn more</a>
-
+
        </li>
-
// x/y positions
+
      </ul>
-
this.x = 200;
+
    </div>
-
this.y = 100;
+
<div class="container">
-
+
        <h3><b>Introduction</b></h3><hr>
-
this.radius = 40;
+
        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.  
-
+
How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
-
// How many wings/blades or whatever you want to call it
+
        <p style="text-align:center"><a class="btn btn-large btn-info" href="https://2013.igem.org/Team:OUC-China/Overview">Learn more</a></p>
-
this.wing_count = 3;
+
</div>
-
// Math.PI*2 === 360 degrees
+
<!--footer-->
-
// 360 / 3 = 120 degrees
+
<footer>
-
// So every 120 degrees will have 1 hand/blade
+
<div class="container">
-
// of 60 degrees (half of 120)
+
<div class="row">
-
this.steps = Math.PI*2 / this.wing_count;
+
<div class="col-md-1"></div>
-
+
<div class="col-md-6 footerleft">
-
this.color = 'hsl(0,100%,50%)';
+
  <h3 class="footer-title" style="color:#bdc1c5;">About</h3>
-
// We can try changing color after EVERY rotation
+
  <p>Thanks to:</p>
-
// would be cool!
+
  <p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Bootstrap&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Flat-ui&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Unslider</p>
-
this.hue = 0; // initial hue
+
  <p>Designed and built by <a href="http://copypeng.com">@PengYong</a> and <a href="http://www.renren.com/345094399/profile?ref=opensearch_normal#notice">@ZhengYuchen.</a></p>
-
// This is the target hue we'll reach
+
  <p>Code licensed under Apache License v2.0</p>
-
// during (at start) the first rotation
+
</div>
-
this.hue_target = parseInt( Math.random()*360 );
+
<div class="col-md-4">
-
+
  <div class="footer-banner">
-
// Lets mess with the angle now
+
    <h3 class="footer-title">Contact US</h3>
-
this.angle = 0;
+
    <ul>
-
// Manipulating speed - seems good!
+
      <li>E-mail:<a href="mailto:oucigem@163.com"> oucigem@163.com</a></li>
-
this.rotation_speed = 0.06;
+
      <li><a href="http://oucast.com"> Official website</a></li>
-
+
      <li><a href="http://www.renren.com/oast"> Find us on Renren</a></li>
-
this.draw = function(ctx) {
+
      <li><a href="http://weibo.com/u/2805858363"> Find us on Weibo</a></li>
-
+
      <li><a href="https://www.google.com/maps?q=+36.062884,+120.335542&sll=36.1360706,120.3648376&sspn=0.36606958894334163,0.9386959091014536&t=m&z=16">Find us on Google Map</a></li>
-
// But we want to do this after every rotation
+
    </ul>
-
if (this.angle > Math.PI*2) {
+
  </div>
-
// Kind of reset the angle when its more
+
</div>
-
// than 360 degrees.
+
<div class="col-md-1"></div>
-
this.angle -= Math.PI*2;
+
</div>
-
+
</div>
-
// Choose a random hue target now
+
</footer>
-
this.hue_target = parseInt( Math.random()*360 );
+
<script src="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/js/bootstrap.min.js"></script>
-
+
<script src="http://unslider.com/unslider.min.js"></script>
-
// ... and we made it :)
+
<script>
-
}
+
$(function() {
-
+
    $('.banner').unslider();
-
// You notice initially there's a color change ?
+
});
-
// Why this (hue_target - hue) * random_numer ?
+
-
// Well, we basically measure the distance that we
+
-
// have to travel, from initial to target and multiple
+
-
// that by an "easing" factor.
+
-
// This causes "easing" effect when changing from initial
+
-
// to target color, i.e., it starts with high speed
+
-
// and as it progresses, the spead decreases until
+
-
// it reaches the target.
+
-
// We just learnt how to implement Easing in html5 canvas!
+
-
this.hue += (this.hue_target - this.hue) * 0.05;
+
-
this.color = 'hsl(' + this.hue + ',100%,50%)';
+
-
+
-
ctx.strokeStyle = this.color;
+
-
ctx.fillStyle = this.color;
+
-
// Setting a line width
+
-
ctx.lineWidth = 15;
+
-
+
-
// Time to rotate this thing
+
-
// Since ctx.rotate() will rotate the entire 2D context
+
-
// and not just the arc's, we'll have to save
+
-
// our current drawing state into the stack.
+
-
ctx.save();
+
-
+
-
// as you can see entire context was rotated
+
-
// we'll fix this by first translating
+
-
// The x/y values that we pass to translate()
+
-
// is the same as the ones we passed to ctx.arc below
+
-
ctx.translate(this.x, this.y);
+
-
+
-
// The entire thing became a circle :P
+
-
// cuz we never cleared after each rendering of frame
+
-
this.angle += this.rotation_speed;
+
-
ctx.rotate(this.angle);
+
-
 
+
-
+
-
for (var i = 0; i < this.wing_count; i++) {
+
-
ctx.beginPath();
+
-
+
-
// For arc, the new x/y pos will be 0,0
+
-
ctx.arc(
+
-
0,
+
-
0,
+
-
this.radius,
+
-
i*this.steps,
+
-
i*this.steps + this.steps/2,
+
-
false
+
-
);
+
-
+
-
ctx.stroke();
+
-
ctx.closePath();
+
-
}
+
-
+
-
// ... and restore here
+
-
ctx.restore();
+
-
+
-
// A rounded body in the center
+
-
ctx.beginPath();
+
-
ctx.fillStyle = '#5aaaff';
+
-
ctx.arc(this.x, this.y, 20, 0, Math.PI*2, false);
+
-
ctx.fill();
+
-
ctx.closePath();
+
-
};
+
-
};
+
-
 
+
-
// Create a spaceship object
+
-
var ship = new SpaceShip();
+
-
// Setting proper x/y to center it
+
-
ship.x = W/2;
+
-
ship.y = H/2;
+
-
+
-
(function renderFrame() {
+
-
window.requestAnimationFrame(renderFrame);
+
-
+
-
// Magik! We dont need so much speed though...
+
-
ctx.clearRect(0,0,W,H);
+
-
ship.draw(ctx);
+
-
// 在canvas上绘制标题文本 
+
-
// 字号为60px，字体为impact 
+
-
ctx.font = "35px arial";
+
-
// 将文本设为居中对齐 
+
-
ctx.textAlign = 'center'; 
+
-
ctx.fillText("OUC-China is coming!", 680, 500);
+
-
}());
+
-
}());
+
</script>
</script>
 +
<script>
 +
      if(window.chrome) {
 +
        $('.banner li').css('background-size', '100% 100%');
 +
      }
 +
      $('.banner').unslider({
 +
        speed: 500,
 +
        delay: 40000,
 +
        keys: true,
 +
        fluid: true,
 +
        dots: true
 +
      });     
 +
    </script>
</body>
</body>
-
<div>
+
</html>
-
<p><a href="/Team:OUC-China/project">P</a></p>
+
-
</div>
+
-
<html>
+

Latest revision as of 03:38, 28 September 2013

    Features Of Our Project

  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

    Learn more
  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

    Learn more
  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

    Learn more
  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

    Learn more

Introduction

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

Learn more

Retrieved from "http://2013.igem.org/Team:OUC-China"