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Team:Paris Saclay/Notebook/August/23
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===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''==== | ===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''==== | ||
| - | ===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of | + | ===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''==== |
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* Well 1 : 6µL DNA Ladder | * Well 1 : 6µL DNA Ladder | ||
| - | * Well 2 : 5µL of | + | * Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI+1µl of 6X loading dye |
* Gel : 1% | * Gel : 1% | ||
|} | |} | ||
Revision as of 08:59, 30 September 2013
Contents |
Notebook : August 23
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3
1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI
| ]] |
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Expect sizes :
- PSB3K3 : 2750 bp
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We can see anything. The digestion did work. |
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