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Team:Paris Saclay/Notebook/July/18
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| + | ='''Notebook : July 18'''= | ||
| + | |||
| + | =='''Lab work'''== | ||
| + | |||
| + | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| + | |||
| + | ===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''==== | ||
| + | |||
| + | ===='''1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI'''==== | ||
| + | |||
| + | Abdou, Anaïs | ||
| + | |||
| + | Used quantities : | ||
| + | * Buffer Dream Taq : 5µL | ||
| + | * dNTP : 1µL | ||
| + | * Oligo VF : 2µL | ||
| + | * Oligo VR : 2µL | ||
| + | * H2O : 37.75µL | ||
| + | * DNA : 2µL | ||
| + | * Dream Taq : 0.25µL | ||
| + | |||
| + | PCR program : | ||
| + | |||
| + | [[File:PsPCR1807.jpg|400px]] | ||
| + | |||
| + | |||
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Latest revision as of 09:47, 30 September 2013
Contents |
Notebook : July 18
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003, BBa_K1155007
1 - PCR of BBa_B0015, BBa_B0017 digested by EcoRI/PstI
Abdou, Anaïs
Used quantities :
- Buffer Dream Taq : 5µL
- dNTP : 1µL
- Oligo VF : 2µL
- Oligo VR : 2µL
- H2O : 37.75µL
- DNA : 2µL
- Dream Taq : 0.25µL
PCR program :
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