Team:TU-Munich/Notebook/Materials

From 2013.igem.org

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==Cell lines==
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*''E. coli'' XL-1 Blue (for molecular cloning)
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*''E. coli'' BL21 (for cytoplasmatic protein production
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*''P. patens'' clone 11 obtained from Prof. Reski (Freiburg University)
==Reagents==
==Reagents==
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! Location
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| 2-Mercaptoethanol
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| AppliChem GmbH
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| Darmstadt, Germany
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Revision as of 23:47, 30 September 2013


Cell lines

  • E. coli XL-1 Blue (for molecular cloning)
  • E. coli BL21 (for cytoplasmatic protein production
  • P. patens clone 11 obtained from Prof. Reski (Freiburg University)

Reagents

Chemicals

Reagent Supplier Location
2-Mercaptoethanol AppliChem GmbH Darmstadt, Germany

Kits and Standards

Plastic Disposables

Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'
TEV_rv 5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'
TEV_t387c_fw 5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'
TEV_t387c_rv 5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'
N_split_TEV_fw 5'- ATTCGCGGCCGCTTCTAG - 3'
N_split_TEV_rv 5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'
C_split_TEV_fw 5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'
C_split_TEV_rv 5'- CCGCTACTAGTATTAACCGG - 3'
PIF6_2-100_fw 5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'
PIF6_2-100_rv 5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'
MinMCS_fo 5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
MinMCS_rev 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'
Bpu_SDMI_rev 5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'
Bpu_SDMII_fo 5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'
Bpu_SDMII_re 5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'
Bpu_SDMIII_fo 5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'
Bpu_SDMIII_re 5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'
Bpu_SDMIV_fo 5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'
Bpu_SDMIV_re 5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
p Act_for 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'
pAct_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'
t35S_for 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'
t35S_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'
Npt_for 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'
Npt_rev 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'
miniMCSII_fw 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
miniMCSII_rv 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'
SpyTag_fw 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'
SpyTag_rv 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'
PIF3(2-100)_fw 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'
PIF3(2-100)_rv 5'- CCGCTACTAGTATTAACCGG - 3'
NucA_fw 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'
NucA_rv 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'
AlcR_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'
AlcR_rv 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'
SQ_pAutoRex8_1 5'- CGGGGCATCACAGCGTCG - 3'
SQ_pAutoRex8_2 5'- TATCGGTAACCGAGTCCG - 3'
AlcR_QCI_fw 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'
AlcR_QCI_rv 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'
AlcR_QCII_fw 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'
AlcR_QCII_rv 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'
AlcR_QCIII_fw 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'
AlcR_QCIII_rv 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'
AlcR_QCIV_fw 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'
AlcR_QCIV_rv 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_fw 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_rv 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'
NPT_QCI_fw 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'
NPT_QCI_rv 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'
NPT_QCII_fw 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'
NPT_QCII_rv 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'
IRES_polio_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'
IRES_polio_rv 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'
BPU_SDMI_r_for 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'
BPU_SDMI_r_rev 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'
Strep-TEV_for 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'
Strep-TEV_rev 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'
SQ_pluc-Actin_1 5'- CCGACTCAAATACAGATATGC - 3'
SQ_pluc-Actin_2 5'- CTCGGATGTAGATCTGCG - 3'
pActin_QC_ins_fw 5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC
ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'
pActin_QC_ins_rv 5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT
GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'
pActin_Oryza_fw 5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'
pActin_Oryza_rv 5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'
GGGGSx5_TEV_fw 5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG
CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
GGGGSx5_TEV_rv 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC
GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'
mMCSII_fw 5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'
mMCSII_rv 5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'
FluA_tripmut_fw 5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'
FluA_tripmut_rv 5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'
pActin_QCI_fw 5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'
pActin_QCI_rv 5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'
Alc R fw Seq. 5'- CAGAGCACAATTGTCCATAC - 3'
Alc R rv Seq. 5'- TCGCGGTCGATGCTCTCC - 3'
pActin_QCins_fw 5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC
CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'
pActin_QCins_rv 5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG
TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'
PP1_for 5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'
PP1_rev 5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'
PhyB fw Seq 5'- GCGATTTCTCAGTTACAGGC - 3'
PhyB rv Seq. 5'- CTCAAAGCACGAGAAAGAAGC - 3'
pAct_ins_fw 5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'
pAct_ins_rv 5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'
PppAct_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'
PppAct_rv 5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'
PppActin_QC1_fw 5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'
PppActin_QC1_rv 5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'
PppActin_QC2_fw 5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'
PppActin_QC2_rv 5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'
PppActin_fw_corr 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'
SYFP2_fw 5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'
SYFP2_rv 5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'
eCFP_fw 5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'
eCFP_rv 5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'
G-TEV-site-G_fw 5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
G-TEV-site-G_fw 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'
PppActin_seq_fw 5'- CATGTCCCAGGGCCCTAG - 3'

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

  1. http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.


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