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Team:TU-Delft/Notebook/2013/09/26/
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2. Restriction digestions was started for : <br> | 2. Restriction digestions was started for : <br> | ||
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3. The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br> | 3. The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br> | ||
5. Ligation is done for : <br> | 5. Ligation is done for : <br> | ||
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| - | Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<<br> | + | Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<<br><br> |
6. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br> | 6. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br> | ||
Revision as of 18:09, 1 October 2013
Notebook
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26th of September
Lab work
1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ.
2. Restriction digestions was started for :
pTet cI TT pcI Ulp pSB1C3 with S+P
3. The membrane experiment is being carried out. To know more click here
5. Ligation is done for :
Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<
6. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue.
