Team:TU-Delft/Notebook/2013/09/26/

From 2013.igem.org

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Revision as of 18:11, 1 October 2013

Notebook

26th of September

Lab work

1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ.

2. Restriction digestions was started for : pTet cI TT pcI Ulp pSB1C3 with S+P

3. The membrane experiment is being carried out. To know more click here
4. Ligation is done for : Lysis (E+X) and pTet cI TT pcI Ulp (E+S)

5. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue.

@@ Line 25: / Line 25: @@
 </left><br>
-. Restriction digestions was started for : <br>
+. Restriction digestions was started for : pTet cI TT pcI Ulp pSB1C3 with S+P <br>
-<br>
-pTet cI TT pcI Ulp pSB1C3 with S+P <br>
 <left>
 <img src="https://static.igem.org/mediawiki/2013/d/d1/130926_Rd2.jpg" height=400px width="600">
-</left><br>
+</left><br><br>
 . The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br>
-. Ligation is done for : <br>
+. Ligation is done for :  Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<br><br>
-Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<br><br>
 . Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br>

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