Team:TU-Delft/Notebook/2013/09/27/

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1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ. <br><br>
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1. The construct pT7 SUMO Peptide was induced with IPTG and a SDS page was run to look for expression of SUMO Peptides.<br>
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Inoculated  colonies 1 and 4.<br>
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2. The construct with pBAD Receiver GFP was induced with 0.1 % Arabinose and after 1 hour induced with AIP's. This was analysed on FACS for GFP Signals. The control was Const GFP and BL21 cells. But the experiment was a failure as the construct itself was faulty. <br>
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2. Restriction digestions was started for : <br>
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pTet cI TT pcI Ulp pSB1C3 with S+P <br>
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3. Colony PCR was done on some colonies seen on the plates for pTet cI TT pcI Ulp lysis pSB1C3.<br>
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<img src="https://static.igem.org/mediawiki/2013/d/d1/130926_Rd2.jpg" height=400px width="600">
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4. Due to repeated failure of the pTet cI TT pcI Ulp Lysis construct ligation for pTet cI TT pcI Ulp with GFP behind it was started: <br>
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3. The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br>
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5. Ligation is done for : <br>
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Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<<br>
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GFP (X+P) and pTet cI TT pcI Ulp (S+P)<br>
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6. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br>
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Revision as of 18:19, 1 October 2013

Notebook

June
SunMonTueWedThuFriSat
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
SunMonTueWedThuFriSat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31










August
SunMonTueWedThuFriSat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
SunMonTueWedThuFriSat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30













27th of September


Lab work

1. The construct pT7 SUMO Peptide was induced with IPTG and a SDS page was run to look for expression of SUMO Peptides.
2. The construct with pBAD Receiver GFP was induced with 0.1 % Arabinose and after 1 hour induced with AIP's. This was analysed on FACS for GFP Signals. The control was Const GFP and BL21 cells. But the experiment was a failure as the construct itself was faulty.
3. Colony PCR was done on some colonies seen on the plates for pTet cI TT pcI Ulp lysis pSB1C3.

4. Due to repeated failure of the pTet cI TT pcI Ulp Lysis construct ligation for pTet cI TT pcI Ulp with GFP behind it was started:

GFP (X+P) and pTet cI TT pcI Ulp (S+P)

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