Team:TU-Munich/Project/Overview

From 2013.igem.org

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===''PHyscomitrella''===
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===''Physcomitrella''===
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 2:]]
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As next point we introduce ''Physcomitrella patens'' as a nearly new chassis to iGEM. We describe advantages and possiblilities and describe our expression strategy ([https://2013.igem.org/Team:TU-Munich/Project/Physcomitrella Read more]).
As next point we introduce ''Physcomitrella patens'' as a nearly new chassis to iGEM. We describe advantages and possiblilities and describe our expression strategy ([https://2013.igem.org/Team:TU-Munich/Project/Physcomitrella Read more]).
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===Localisation===
===Localisation===
[[File:TUM13 Icon.jpg|thumb|right|200px| Figure 3:]]
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Revision as of 14:44, 2 October 2013


Project Overview

In the 2013 competition the TU Munich iGEM team has developed a transgenic moss filter which is capable to reduct the the contamination of aquatic ecosystems with xenobiotics. The topic of remediation using transgenic organisms is present in every year of iGEM and we want to take this idea to the next level. For this reason we decided to use a photoautotrophic chassis which we enabled for the iGEM competition. The following section gives an overview over the theoretic background of our project.


Phytoremediation

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Figure 1:

Phytoremediation describes the treatment of environmental problems through the use of plants. Here we identified problematic substances and described basic principles (Read more).

Physcomitrella

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Figure 2:

As next point we introduce Physcomitrella patens as a nearly new chassis to iGEM. We describe advantages and possiblilities and describe our expression strategy (Read more).

Localisation

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Figure 3:

As the principle of action for the effectors responsible for the Phytoremediation are very different it is necessary to have a expression system which is able to accomplish cytosolic, secreted, receptor bound localisation of proteins (Read more).

BioDegradation

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Figure 4:

BioDegradation describes the application of enzymatic catalysis for the degradation of problematic xenobiotics using an erythromycin esterase, a catechol dioxigenase and a laccase (Read more).


Safety

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Figure 5:

Our safety page describes our teams discussion concerning safty issues, especially a safety evaluation of our BioBricks and Physcomitralla including all constructed GM-plants (Read more).

Kill-switch

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Figure 6:

In order to restrict the growth of transgenic moss to a controlled environment we developed a kill-switch which becomes triggered by sunlight. The GM-moss can only be grown in places where redlight has been filtered out (Read more).



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