Team:TU-Munich/Results/BioBricks

From 2013.igem.org

(Difference between revisions)

Revision as of 22:04, 2 October 2013

Sendbox

Igkappa-GFP

<groupparts>iGEM2013 TU-Munich</groupparts> <groupparts>iGEM2013 Dundee</groupparts>

Figure 1:Successful preparing of the biobrick sendbox

Figure 2:One of our biobrick sendboxes

BioBricks we improved

Some BioBricks which were already present in the parts registry were converted from RFC[10] to RFC[25] by PCR. The purpose of this improvement was that we introduced with our project the possiblility to dictate the cellular localisation of proteins (cytosolic, secreted and receptor bound). For this purpose the open reading frame of the protein has to be fused to a N-terminal signal peptide (for secretion) or has to be inserted into the extracellular domain of a synthetic receptor. We converted the following BioBricks to RFC[25]:

Laccase <partinfo>BBa_K863000</partinfo> was improved to the BioBrick <partinfo>BBa_K1159002</partinfo>
Protein phosphatase 1

For the fluoresceine binding anticalin

FluA

BioBricks we characterized

LMU-GFP

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.

@@ Line 10: / Line 10: @@
 *Igkappa-GFP
 <groupparts>iGEM2013 TU-Munich</groupparts>
+<groupparts>iGEM2013 Dundee</groupparts>
 [[File:TUM13 Biobrick_1.png|thumb|left|350px|Figure 1:Successful preparing of the biobrick sendbox]]
@@ Line 15: / Line 16: @@
 == BioBricks we improved ==
-*Laccase
+Some BioBricks which were already present in the parts registry were converted from RFC[10] to RFC[25] by PCR. The purpose of this improvement was that we introduced with our project the possiblility to dictate the cellular localisation of proteins (cytosolic, secreted and receptor bound). For this purpose the open reading frame of the protein has to be fused to a N-terminal signal peptide (for secretion) or has to be inserted into the extracellular domain of a synthetic receptor. We converted the following BioBricks to RFC[25]:
-*FluA
+*Laccase <partinfo>BBa_K863000</partinfo> was improved to the BioBrick <partinfo>BBa_K1159002</partinfo>
-*PP1
+*Protein phosphatase 1
+For the fluoresceine binding anticalin
+*FluA
 == BioBricks we characterized ==
-*stress-inducible
+*
 *LMU-GFP