Team:TU-Delft/Protocol2

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<div style="margin-left:30px;margin-right:30px; width:900px;float:left;"> 
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<h2 align="center">Protocols</h2>
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Our project deals with <i>E.coli</i> cells which sense Auto-inducing peptides (AIPs) from the <i>Staphylococcus aureus</i> and starts producing Antimicrobial peptides in order to kill the <i>Staphylococcus aureus</i>. Different protocols used during the project are described below.
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  <li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_1" style="text-decoration: none""><font color="#0080FF" size="3">Transforming Parts from Distribution kit</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol2" style="text-decoration: none""><font color="#0080FF" size="3">Growing the Single Colonies from the Agar Plates</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_3" style="text-decoration: none""><font
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color="#0080FF" size="3"> Making glycerol stocks</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_4" style="text-decoration: none""><font
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color="#0080FF" size="3"> Miniprep Protocol</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_5" style="text-decoration: none""><font
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color="#0080FF" size="3"> Restriction digestion</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_6" style="text-decoration: none""><font
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color="#0080FF" size="3"> Ligation</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_7" style="text-decoration: none"" ><font
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color="#0080FF" size="3"> Gel Extraction Procedure</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_8" style="text-decoration: none""><font
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color="#0080FF" size="3">  PCR Purification</font></a> </li>
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<li> <a href="https://2013.igem.org/Team:TU-Delft/Protocol_9" style="text-decoration: none""><font
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color="#0080FF" size="3">  Tricine Gels</font></a> </li>
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<h2 align="center">Growing the Single Colonies from the Agar Plates</h2>
<h2 align="center">Growing the Single Colonies from the Agar Plates</h2>
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<h4 align="left">Prodecure:</h4>
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<h4 align="left">Procedure:</h4>
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<li> Prepare flask with LB Broth in it. Add the appropriate antibiotic needed.</li>
<li> Prepare flask with LB Broth in it. Add the appropriate antibiotic needed.</li>

Latest revision as of 15:26, 3 October 2013



Protocols

Our project deals with E.coli cells which sense Auto-inducing peptides (AIPs) from the Staphylococcus aureus and starts producing Antimicrobial peptides in order to kill the Staphylococcus aureus. Different protocols used during the project are described below.



Growing the Single Colonies from the Agar Plates

Requirements:

  1. LB Broth
  2. Pipettes
  3. Antibiotics
  4. Sterile flasks

Procedure:

  1. Prepare flask with LB Broth in it. Add the appropriate antibiotic needed.
  2. Select the single colony using the pipette tip from the Agar plate, which contains the bacterial cells.
  3. Inoculate the LB broth with the bacterial cells.
  4. Grow the culture for 14-16 hours.

    5. Next day prepare glycerol stocks and carry out a miniprep protocol to extract the desired plasmid from the bacterial cells.

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