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Team:Paris Saclay/Protocols
From 2013.igem.org
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='''Protocols'''= | ='''Protocols'''= | ||
| - | [[Team:Paris_Saclay/preparation|Preparation of | + | [[Team:Paris_Saclay/preparation|Preparation of super competent ''E.coli'' cells ]] |
| - | [[Team:Paris_Saclay/Protocols/Transformation|Transformation of | + | [[Team:Paris_Saclay/Protocols/Transformation|Transformation of super competent ''E.coli'' cells]] |
[[Team:Paris_Saclay/extraction|Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue ]] | [[Team:Paris_Saclay/extraction|Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue ]] | ||
| Line 21: | Line 21: | ||
[[Team:Paris_Saclay/mini_maxi|Plasmidic DNA Mini and Maxi preparation ]] | [[Team:Paris_Saclay/mini_maxi|Plasmidic DNA Mini and Maxi preparation ]] | ||
| - | [[Team:Paris_Saclay/protocols/pcr|PCR for the genomic DNA | + | [[Team:Paris_Saclay/protocols/pcr|PCR for the bacterial genomic DNA ]] |
[[Team:Paris_Saclay/electro|Electro-elution ]] | [[Team:Paris_Saclay/electro|Electro-elution ]] | ||
| - | [[Team:Paris_Saclay/gibson|Gibson Assembly ]] | + | [[Team:Paris_Saclay/gibson|Gibson Assembly method ]] |
| - | [[Team:Paris_Saclay/gel|Electrophoresis | + | [[Team:Paris_Saclay/gel|Gel Electrophoresis ]] |
[[Team:Paris_Saclay/ethanol|Ethanol Precipitation ]] | [[Team:Paris_Saclay/ethanol|Ethanol Precipitation ]] | ||
Revision as of 15:49, 3 October 2013
Protocols
Preparation of super competent E.coli cells
Transformation of super competent E.coli cells
Extraction of the Genomic DNA from Bacteria by using NucleoSpin® Tissue
Plasmidic DNA Mini and Maxi preparation
PCR for the bacterial genomic DNA
[http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
Protocols written by Sheng (the 4 first), Nadia (Transduction) and Abdou
