Team:TU-Delft/Notebook/2013/07/08/

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Latest revision as of 08:43, 4 October 2013

1. Restriction digestion of pBAD dor pSB1C3 backbone.
2. Restreaking Staphyllococcus delphini , Bacillus subtilis and DH5-alpha cells.

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-<p>  1. Biobricks from the iGEM registry were streaked on Agar plates.<br>
+<p> 1. Restriction digestion of pBAD dor pSB1C3 backbone. <br>
+. Restreaking <i>Staphyllococcus delphini , Bacillus subtilis </i> and DH5-alpha cells.  <br>
-. Transformation of the pTET backbone with Lysis cassette, which was done on 5/7/13.<br>
-. Analysis of the Gel electrophoresis in lab meeting. pBAD and pT7 was not that good. AIP sender and receiver also shows contamination of wells. <br>
-. The biobricks: pBAD, pT7, GFP, AIP sender(Agr A: AgrC). AIP receiver(AgrB: AgrD), pTET:lysis were grown on Agar plates. But the negative control is not convincing, so Gel electrophoresis is run on random colonies from the plates to check. <br>
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