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Team:TU-Delft/Notebook/2013/09/26/
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1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ. <br><br> | 1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ. <br><br> | ||
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| - | <img src=" | + | <img src="https://static.igem.org/mediawiki/2013/4/4e/2013-10-03_21.01.09.jpg" height=250px width="600"> |
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| - | 2. Restriction digestions was started for : | + | 2. Restriction digestions was started for : pTet cI TT pcI Ulp pSB1C3 with S+P <br> |
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| - | pTet cI TT pcI Ulp pSB1C3 with S+P <br> | + | |
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<img src="https://static.igem.org/mediawiki/2013/d/d1/130926_Rd2.jpg" height=400px width="600"> | <img src="https://static.igem.org/mediawiki/2013/d/d1/130926_Rd2.jpg" height=400px width="600"> | ||
| - | </left><br> | + | </left><br><br> |
3. The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br> | 3. The membrane experiment is being carried out. To know more click <a href="https://2013.igem.org/Team:TU-Delft/FinalApplication" target="blank"> here</a> <br> | ||
| - | 4. Ligation is done for : | + | 4. Ligation is done for : Lysis (E+X) and pTet cI TT pcI Ulp (E+S)<br> |
| - | + | ||
| - | Lysis (E+X) and pTet cI TT pcI Ulp (E+S) | + | |
5. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br> | 5. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue. <br> | ||
Latest revision as of 09:15, 4 October 2013
Notebook
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| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
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| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 |
26th of September
Lab work
1. Colony PCR for the new pBAD AIP receiver GFP was done, along with PCR of the Bio brick BBa_K1022100 to check if the right biobrick was sent to iGEM HQ.
2. Restriction digestions was started for : pTet cI TT pcI Ulp pSB1C3 with S+P
3. The membrane experiment is being carried out. To know more click here
4. Ligation is done for : Lysis (E+X) and pTet cI TT pcI Ulp (E+S)
5. Transformation is done for pTet cI TT pcI Ulp Lysis in pSB1C3 in XL1 Blue.
