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Team:Paris Saclay/Notebook/August/7
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155003'''==== |
| - | ===='''1 - Tranformation | + | ===='''1 - Sequensis analysis of BBa_K1155003'''==== |
| + | |||
| + | Abdou | ||
| + | |||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DE;" | | ||
| + | The sequence analysis was good. We call our biobrick BBa_K1155003. | ||
| + | |} | ||
| + | |||
| + | ===='''Objective : obtaining BBa_K1155007'''==== | ||
| + | |||
| + | ===='''1 - Ligation of RBS-LacZ-Term in pSB1C3 ==== | ||
| + | |||
| + | Anaïs, Nadia | ||
| + | |||
| + | Used quantities : | ||
| + | * RBS-AmilCP : 2µL | ||
| + | * Term in pSB1C3 : 2.5µL | ||
| + | * Buffer : 1µL | ||
| + | * Ligase : 1µL | ||
| + | * H2O : 3.5µL | ||
| + | |||
| + | ===='''2 - Tranformation BBa_K115507 in DH5αstrain==== | ||
XiaoJing | XiaoJing | ||
| Line 15: | Line 37: | ||
Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | Protocol : [[Team:Paris_Saclay/Protocols/Transformation|Bacterial transformation]] | ||
| - | ===='''Objective : characterize | + | ===='''Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006'''==== |
| + | |||
| + | ===='''1 - Tranduction of Km in MG1655Z1 ==== | ||
| + | |||
| + | Nadia, XiaoJing | ||
| + | |||
| + | Protocol : [[Team:Paris_Saclay/transduction|Transduction]] | ||
| + | |||
| + | Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km. | ||
| + | |||
| + | We did the first step of the protocol : bacteriophage stock grown on BW1328. | ||
| + | |||
| - | = | + | {| border="1" align="center" |
| + | |[[Team:Paris Saclay/Notebook/August/6|<big>Previous day</big>]] | ||
| + | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
| + | |[[Team:Paris Saclay/Notebook/August/8|<big>Next day</big>]] | ||
| + | |} | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Latest revision as of 21:19, 4 October 2013
Notebook : August 7
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155003
1 - Sequensis analysis of BBa_K1155003
Abdou
|
The sequence analysis was good. We call our biobrick BBa_K1155003. |
Objective : obtaining BBa_K1155007
1 - Ligation of RBS-LacZ-Term in pSB1C3
Anaïs, Nadia
Used quantities :
- RBS-AmilCP : 2µL
- Term in pSB1C3 : 2.5µL
- Buffer : 1µL
- Ligase : 1µL
- H2O : 3.5µL
2 - Tranformation BBa_K115507 in DH5αstrain
XiaoJing
Protocol : Bacterial transformation
Objective : characterize BBa_K1155000, BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Tranduction of Km in MG1655Z1
Nadia, XiaoJing
Protocol : Transduction
Wild type strain is MG1655Z1 and our mutant strain is BW1328: Δfnr::Km.
We did the first step of the protocol : bacteriophage stock grown on BW1328.
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