Team:Paris Saclay/Primers

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{{Team:Paris_Saclay/incl_contenu}}
{{Team:Paris_Saclay/incl_contenu}}
 +
 +
='''Primers'''=
{| class="wikitable"
{| class="wikitable"
Line 16: Line 18:
! scope="col"| Number
! scope="col"| Number
! scope="col"| Name
! scope="col"| Name
-
! scope="col"| Sequence
+
! scope="col"| Sequence 5' to 3'
! scope="col"| Organism
! scope="col"| Organism
|-
|-
Line 32: Line 34:
! scope="row"| iPS35
! scope="row"| iPS35
| bphR2_up
| bphR2_up
-
| ATATGAATTCGCGGCCGCTTCTAGATGGAACTGCATGACCTGGA
+
| ATATGAATTCGCGGCCGCTTCTAGA
 +
TGGAACTGCATGACCTGGA
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS36
! scope="row"| iPS36
| bphR2_down
| bphR2_down
-
| ATATCTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGA
+
| ATATCTGCAGCGGCCGCTACTAGTAT
 +
CAATCCGAAAACAGCTCGA
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS37
! scope="row"| iPS37
| bphR1_up
| bphR1_up
-
| ATATGAATTCGCGGCCGCTTCTAGAGATGTCCCTGGGTGACATGCGTG
+
| ATATGAATTCGCGGCCGCTTCTAGAG
 +
ATGTCCCTGGGTGACATGCGTG
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS38
! scope="row"| iPS38
| bphR1_down
| bphR1_down
-
| ATATCTGCAGCGGCCGCTACTAGTAAGCAGTCAATCGGCGGTAGCGC
+
| ATATCTGCAGCGGCCGCTACTAGTAA
 +
GCAGTCAATCGGCGGTAGCGC
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS39
! scope="row"| iPS39
| bphA1_up
| bphA1_up
-
| ATATGAATTCGCGGCCGCTTCTAGAGCGCCAACATCCCACGTGTTTG
+
| ATATGAATTCGCGGCCGCTTCTAGAGC
 +
GCCAACATCCCACGTGTTTG
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS40
! scope="row"| iPS40
| bphA1_down
| bphA1_down
-
| ATATCTGCAGCGGCCGCTACTAGTACCCCCGGATCGCCTCCGGCGTC
+
| ATATCTGCAGCGGCCGCTACTAGTACC
 +
CCCGGATCGCCTCCGGCGTC
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
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! scope="row"| iPS43
! scope="row"| iPS43
| VF2
| VF2
-
| ATTACCGCCTTTGAGTGAGC
+
| TGCCACCTGACGTCTAAGAA
| pSB1C3, E.coli
| pSB1C3, E.coli
|-
|-
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|-
|-
! scope="row"| iPS45
! scope="row"| iPS45
-
| Prefix-prom _nirB  
+
| Prefix-prom_nirB  
-
| TCATGAATTCGCGGCCGCTTCTAGAGCTACGTGCATCTATTTCTAT
+
| TCATGAATTCGCGGCCGCTTCTAGA
 +
GCTACGTGCATCTATTTCTAT
| E.coli
| E.coli
|-
|-
! scope="row"| iPS46
! scope="row"| iPS46
| suffixe-prom_nirB
| suffixe-prom_nirB
-
| TACTCTGCAGCGGCCGCTACTAGTATGCCTCGATTTCTTTTCTAT
+
| TACTCTGCAGCGGCCGCTACTAGTA
 +
TGCCTCGATTTCTTTTCTAT
| E.coli
| E.coli
|-
|-
! scope="row"| iPS47
! scope="row"| iPS47
-
| Prefix-prom _narK
+
| Prefix-prom_narK
-
| TCATGAATTCGCGGCCGCTTCTAGAGACATCGGTAAGGGTAGGGAT
+
| TCATGAATTCGCGGCCGCTTCTAGAG
 +
ACATCGGTAAGGGTAGGGAT
| E.coli
| E.coli
|-
|-
! scope="row"| iPS48
! scope="row"| iPS48
| suffixe-prom_narK
| suffixe-prom_narK
-
| TACTCTGCAGCGGCCGCTACTAGTAAGACACCTCTGATACTCGTT
+
| TACTCTGCAGCGGCCGCTACTAGTAA
 +
GACACCTCTGATACTCGTT
| E.coli
| E.coli
|-
|-
! scope="row"| iPS49
! scope="row"| iPS49
-
| prefix-prom _narG
+
| prefix-prom_narG
-
| TCATGAATTCGCGGCCGCTTCTAGAGGGAATTTACTTTATTTTTCA
+
| TCATGAATTCGCGGCCGCTTCTAGAG
 +
GGAATTTACTTTATTTTTCA
| E.coli
| E.coli
|-
|-
! scope="row"| iPS50
! scope="row"| iPS50
| suffixe-prom_narG
| suffixe-prom_narG
-
| TACTCTGCAGCGGCCGCTACTAGTATTCTCCTGTGGGAGCCTGTC
+
| TACTCTGCAGCGGCCGCTACTAGTAT
 +
TCTCCTGTGGGAGCCTGTC
| E.coli
| E.coli
|-
|-
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| sufix from pSB1C3
| sufix from pSB1C3
| GTACCTGCAGCGGCCGCTACTAGTA
| GTACCTGCAGCGGCCGCTACTAGTA
-
| pSB1C3, E.coli
+
| pSB1C3,E.coli
|-
|-
! scope="row"| iPS53
! scope="row"| iPS53
-
| prefix -RBS-AmilCP
+
| prefix-RBS-AmilCP
-
| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGTGTGATCGCTAAACAAAT
+
| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG
-
| pSB1C3, E.coli
+
AGAAATACTAGATGAGTGTGATCGCTAAACAAAT
 +
| pSB1C3,E.coli
|-
|-
! scope="row"| iPS54
! scope="row"| iPS54
| Bba_BphR2-part1-Gibson-Forward
| Bba_BphR2-part1-Gibson-Forward
-
| GAATTCGCGGCCGCTTCTAGAGATGGAACTGCATGACCTGGAT
+
| GAATTCGCGGCCGCTTCTAGAGATGGAA
 +
CTGCATGACCTGGAT
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
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! scope="row"| iPS57
! scope="row"| iPS57
| Bba_BphR2-part2-Gibson-Reverse
| Bba_BphR2-part2-Gibson-Reverse
-
| CTGCAGCGGCCGCTACTAGTATCAATCCGAAAACAGCTCGAACA
+
| CTGCAGCGGCCGCTACTAGTATCAATCCG
 +
AAAACAGCTCGAACA
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS58
! scope="row"| iPS58
| Bba_RBS-BphR2-part1-Gibson-Forward
| Bba_RBS-BphR2-part1-Gibson-Forward
-
| GAATTCGCGGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGAACTGCATGACCTGGAT
+
| GAATTCGCGGGCCGCTTCTAGAGAAAGAGG
 +
AGAAATACTAGATGGAACTGCATGACCTGGAT
| P. pseuoalcaligenes
| P. pseuoalcaligenes
|-
|-
! scope="row"| iPS59
! scope="row"| iPS59
| Bba_FNR-part1-Gibson-Forward
| Bba_FNR-part1-Gibson-Forward
-
| GAATTCGCGGCCGCTTCTAGAGATGATCCCGGAAAAGCGAAT
+
| GAATTCGCGGCCGCTTCTAGAGATGATCCC
 +
GGAAAAGCGAAT
| E.coli
| E.coli
|-
|-
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! scope="row"| iPS62
! scope="row"| iPS62
| Bba_FNR-part2-Gibson-Reverse
| Bba_FNR-part2-Gibson-Reverse
-
| CTGCAGCGGCCGCTACTAGTATCAGGCAACGTTACGCGTAT
+
| CTGCAGCGGCCGCTACTAGTATCAGGCAAC
 +
GTTACGCGTAT
| E.coli
| E.coli
|-
|-
! scope="row"| iPS63
! scope="row"| iPS63
| Bba_RBS-FNR-part1-Gibson-Forward
| Bba_RBS-FNR-part1-Gibson-Forward
-
| GAATTCGCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGATCCCGGAAAAGCGAATT
+
| GAATTCGCGGCCGCTTCTAGAGAAAGAGGA
 +
GAAATACTAGATGATCCCGGAAAAGCGAATT
| E.coli
| E.coli
|-
|-
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| Suffix-from-pSB1C3-Gibson
| Suffix-from-pSB1C3-Gibson
| TACTAGTAGCGGCCGCTGCAG
| TACTAGTAGCGGCCGCTGCAG
-
| pSB1C3, E.coli
+
| pSB1C3,E.coli
|-
|-
! scope="row"| iPS65
! scope="row"| iPS65
| Prefix-from-pSB1C3-Gibson
| Prefix-from-pSB1C3-Gibson
| CTCTAGAAGCGGCCGCGAATTC
| CTCTAGAAGCGGCCGCGAATTC
-
| pSB1C3, E.coli
+
| pSB1C3,E.coli
|-
|-

Latest revision as of 00:23, 5 October 2013

Labwork Overview Biobricks Protocols Primers Safety

Primers

Number Name Sequence 5' to 3' Organism
iPS33 pfnr_up ATATGAATTCGCGGCCGCTTCTAGAGT E.coli
iPS34 pfnr_down ATATCTGCAGCGGCCGCTACTAG

TAGTTCTTCGCTATTGAGAATAACATG

E.coli
iPS35 bphR2_up ATATGAATTCGCGGCCGCTTCTAGA

TGGAACTGCATGACCTGGA

P. pseuoalcaligenes
iPS36 bphR2_down ATATCTGCAGCGGCCGCTACTAGTAT

CAATCCGAAAACAGCTCGA

P. pseuoalcaligenes
iPS37 bphR1_up ATATGAATTCGCGGCCGCTTCTAGAG

ATGTCCCTGGGTGACATGCGTG

P. pseuoalcaligenes
iPS38 bphR1_down ATATCTGCAGCGGCCGCTACTAGTAA

GCAGTCAATCGGCGGTAGCGC

P. pseuoalcaligenes
iPS39 bphA1_up ATATGAATTCGCGGCCGCTTCTAGAGC

GCCAACATCCCACGTGTTTG

P. pseuoalcaligenes
iPS40 bphA1_down ATATCTGCAGCGGCCGCTACTAGTACC

CCCGGATCGCCTCCGGCGTC

P. pseuoalcaligenes
iPS41 SB-prep-3P-1 GCCGCTGCAGTCCGGCAAAAAA pSB1C3, E.coli
iPS42 SB-prep-2Ea TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS43 VF2 TGCCACCTGACGTCTAAGAA pSB1C3, E.coli
iPS44 VR ATTACCGCCTTTGAGTGAGC pSB1C3, E.coli
iPS45 Prefix-prom_nirB TCATGAATTCGCGGCCGCTTCTAGA

GCTACGTGCATCTATTTCTAT

E.coli
iPS46 suffixe-prom_nirB TACTCTGCAGCGGCCGCTACTAGTA

TGCCTCGATTTCTTTTCTAT

E.coli
iPS47 Prefix-prom_narK TCATGAATTCGCGGCCGCTTCTAGAG

ACATCGGTAAGGGTAGGGAT

E.coli
iPS48 suffixe-prom_narK TACTCTGCAGCGGCCGCTACTAGTAA

GACACCTCTGATACTCGTT

E.coli
iPS49 prefix-prom_narG TCATGAATTCGCGGCCGCTTCTAGAG

GGAATTTACTTTATTTTTCA

E.coli
iPS50 suffixe-prom_narG TACTCTGCAGCGGCCGCTACTAGTAT

TCTCCTGTGGGAGCCTGTC

E.coli
iPS51 Prefix from pSB1C3 CTCTAGAAGCGGCCGCGAATTC pSB1C3, E.coli
iPS52 sufix from pSB1C3 GTACCTGCAGCGGCCGCTACTAGTA pSB1C3,E.coli
iPS53 prefix-RBS-AmilCP CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGAGTGTGATCGCTAAACAAAT

pSB1C3,E.coli
iPS54 Bba_BphR2-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGGAA

CTGCATGACCTGGAT

P. pseuoalcaligenes
iPS55 Bba_BphR2-part1-Gibson-Reverse CAGTTCGTCCTGTAGTGCGG P. pseuoalcaligenes
iPS56 Bba_BphR2-part2-Gibson-Forward CACCGCACTACAGGACGAAC P. pseuoalcaligenes
iPS57 Bba_BphR2-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAATCCG

AAAACAGCTCGAACA

P. pseuoalcaligenes
iPS58 Bba_RBS-BphR2-part1-Gibson-Forward GAATTCGCGGGCCGCTTCTAGAGAAAGAGG

AGAAATACTAGATGGAACTGCATGACCTGGAT

P. pseuoalcaligenes
iPS59 Bba_FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGATGATCCC

GGAAAAGCGAAT

E.coli
iPS60 Bba_FNR-part1-Gibson-Reverse CGGAACTCACGAGGGGAGAA E.coli
iPS61 Bba_FNR-part2-Gibson-Forward TTCTCCCCTCGTGAGTTCCG E.coli
iPS62 Bba_FNR-part2-Gibson-Reverse CTGCAGCGGCCGCTACTAGTATCAGGCAAC

GTTACGCGTAT

E.coli
iPS63 Bba_RBS-FNR-part1-Gibson-Forward GAATTCGCGGCCGCTTCTAGAGAAAGAGGA

GAAATACTAGATGATCCCGGAAAAGCGAATT

E.coli
iPS64 Suffix-from-pSB1C3-Gibson TACTAGTAGCGGCCGCTGCAG pSB1C3,E.coli
iPS65 Prefix-from-pSB1C3-Gibson CTCTAGAAGCGGCCGCGAATTC pSB1C3,E.coli

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Primers"