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Team:Paris Saclay/Primers
From 2013.igem.org
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! scope="col"| Number | ! scope="col"| Number | ||
! scope="col"| Name | ! scope="col"| Name | ||
| - | ! scope="col"| Sequence | + | ! scope="col"| Sequence 5' to 3' |
! scope="col"| Organism | ! scope="col"| Organism | ||
|- | |- | ||
| Line 80: | Line 80: | ||
! scope="row"| iPS43 | ! scope="row"| iPS43 | ||
| VF2 | | VF2 | ||
| - | | | + | | TGCCACCTGACGTCTAAGAA |
| pSB1C3, E.coli | | pSB1C3, E.coli | ||
|- | |- | ||
| Line 89: | Line 89: | ||
|- | |- | ||
! scope="row"| iPS45 | ! scope="row"| iPS45 | ||
| - | | Prefix- | + | | Prefix-prom_nirB |
| TCATGAATTCGCGGCCGCTTCTAGA | | TCATGAATTCGCGGCCGCTTCTAGA | ||
GCTACGTGCATCTATTTCTAT | GCTACGTGCATCTATTTCTAT | ||
| Line 101: | Line 101: | ||
|- | |- | ||
! scope="row"| iPS47 | ! scope="row"| iPS47 | ||
| - | | Prefix- | + | | Prefix-prom_narK |
| TCATGAATTCGCGGCCGCTTCTAGAG | | TCATGAATTCGCGGCCGCTTCTAGAG | ||
ACATCGGTAAGGGTAGGGAT | ACATCGGTAAGGGTAGGGAT | ||
| Line 113: | Line 113: | ||
|- | |- | ||
! scope="row"| iPS49 | ! scope="row"| iPS49 | ||
| - | | prefix- | + | | prefix-prom_narG |
| TCATGAATTCGCGGCCGCTTCTAGAG | | TCATGAATTCGCGGCCGCTTCTAGAG | ||
GGAATTTACTTTATTTTTCA | GGAATTTACTTTATTTTTCA | ||
| Line 135: | Line 135: | ||
|- | |- | ||
! scope="row"| iPS53 | ! scope="row"| iPS53 | ||
| - | | prefix -RBS-AmilCP | + | | prefix-RBS-AmilCP |
| CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG | | CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG | ||
AGAAATACTAGATGAGTGTGATCGCTAAACAAAT | AGAAATACTAGATGAGTGTGATCGCTAAACAAAT | ||
Latest revision as of 00:23, 5 October 2013
Primers
| Number | Name | Sequence 5' to 3' | Organism |
|---|---|---|---|
| iPS33 | pfnr_up | ATATGAATTCGCGGCCGCTTCTAGAGT | E.coli |
| iPS34 | pfnr_down | ATATCTGCAGCGGCCGCTACTAG
TAGTTCTTCGCTATTGAGAATAACATG | E.coli |
| iPS35 | bphR2_up | ATATGAATTCGCGGCCGCTTCTAGA
TGGAACTGCATGACCTGGA | P. pseuoalcaligenes |
| iPS36 | bphR2_down | ATATCTGCAGCGGCCGCTACTAGTAT
CAATCCGAAAACAGCTCGA | P. pseuoalcaligenes |
| iPS37 | bphR1_up | ATATGAATTCGCGGCCGCTTCTAGAG
ATGTCCCTGGGTGACATGCGTG | P. pseuoalcaligenes |
| iPS38 | bphR1_down | ATATCTGCAGCGGCCGCTACTAGTAA
GCAGTCAATCGGCGGTAGCGC | P. pseuoalcaligenes |
| iPS39 | bphA1_up | ATATGAATTCGCGGCCGCTTCTAGAGC
GCCAACATCCCACGTGTTTG | P. pseuoalcaligenes |
| iPS40 | bphA1_down | ATATCTGCAGCGGCCGCTACTAGTACC
CCCGGATCGCCTCCGGCGTC | P. pseuoalcaligenes |
| iPS41 | SB-prep-3P-1 | GCCGCTGCAGTCCGGCAAAAAA | pSB1C3, E.coli |
| iPS42 | SB-prep-2Ea | TGCCACCTGACGTCTAAGAA | pSB1C3, E.coli |
| iPS43 | VF2 | TGCCACCTGACGTCTAAGAA | pSB1C3, E.coli |
| iPS44 | VR | ATTACCGCCTTTGAGTGAGC | pSB1C3, E.coli |
| iPS45 | Prefix-prom_nirB | TCATGAATTCGCGGCCGCTTCTAGA
GCTACGTGCATCTATTTCTAT | E.coli |
| iPS46 | suffixe-prom_nirB | TACTCTGCAGCGGCCGCTACTAGTA
TGCCTCGATTTCTTTTCTAT | E.coli |
| iPS47 | Prefix-prom_narK | TCATGAATTCGCGGCCGCTTCTAGAG
ACATCGGTAAGGGTAGGGAT | E.coli |
| iPS48 | suffixe-prom_narK | TACTCTGCAGCGGCCGCTACTAGTAA
GACACCTCTGATACTCGTT | E.coli |
| iPS49 | prefix-prom_narG | TCATGAATTCGCGGCCGCTTCTAGAG
GGAATTTACTTTATTTTTCA | E.coli |
| iPS50 | suffixe-prom_narG | TACTCTGCAGCGGCCGCTACTAGTAT
TCTCCTGTGGGAGCCTGTC | E.coli |
| iPS51 | Prefix from pSB1C3 | CTCTAGAAGCGGCCGCGAATTC | pSB1C3, E.coli |
| iPS52 | sufix from pSB1C3 | GTACCTGCAGCGGCCGCTACTAGTA | pSB1C3,E.coli |
| iPS53 | prefix-RBS-AmilCP | CATAGAATTCGCGGCCGCTTCTAGAGAAAGAGG
AGAAATACTAGATGAGTGTGATCGCTAAACAAAT | pSB1C3,E.coli |
| iPS54 | Bba_BphR2-part1-Gibson-Forward | GAATTCGCGGCCGCTTCTAGAGATGGAA
CTGCATGACCTGGAT | P. pseuoalcaligenes |
| iPS55 | Bba_BphR2-part1-Gibson-Reverse | CAGTTCGTCCTGTAGTGCGG | P. pseuoalcaligenes |
| iPS56 | Bba_BphR2-part2-Gibson-Forward | CACCGCACTACAGGACGAAC | P. pseuoalcaligenes |
| iPS57 | Bba_BphR2-part2-Gibson-Reverse | CTGCAGCGGCCGCTACTAGTATCAATCCG
AAAACAGCTCGAACA | P. pseuoalcaligenes |
| iPS58 | Bba_RBS-BphR2-part1-Gibson-Forward | GAATTCGCGGGCCGCTTCTAGAGAAAGAGG
AGAAATACTAGATGGAACTGCATGACCTGGAT | P. pseuoalcaligenes |
| iPS59 | Bba_FNR-part1-Gibson-Forward | GAATTCGCGGCCGCTTCTAGAGATGATCCC
GGAAAAGCGAAT | E.coli |
| iPS60 | Bba_FNR-part1-Gibson-Reverse | CGGAACTCACGAGGGGAGAA | E.coli |
| iPS61 | Bba_FNR-part2-Gibson-Forward | TTCTCCCCTCGTGAGTTCCG | E.coli |
| iPS62 | Bba_FNR-part2-Gibson-Reverse | CTGCAGCGGCCGCTACTAGTATCAGGCAAC
GTTACGCGTAT | E.coli |
| iPS63 | Bba_RBS-FNR-part1-Gibson-Forward | GAATTCGCGGCCGCTTCTAGAGAAAGAGGA
GAAATACTAGATGATCCCGGAAAAGCGAATT | E.coli |
| iPS64 | Suffix-from-pSB1C3-Gibson | TACTAGTAGCGGCCGCTGCAG | pSB1C3,E.coli |
| iPS65 | Prefix-from-pSB1C3-Gibson | CTCTAGAAGCGGCCGCGAATTC | pSB1C3,E.coli |
