Team:Paris Saclay/Notebook/August/23

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(1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI)
 
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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in PSB3K3'''====
+
===='''Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3'''====
===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''====
===='''1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI'''====
{|
{|
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| style="width:350px;border:1px solid black;" |]]
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| style="width:350px;border:1px solid black;" |[[File:Psgel2308.jpg|200px]]
| style="width:350px;border:1px solid black;vertical-align:top;" |
| style="width:350px;border:1px solid black;vertical-align:top;" |
* Well 1 : 6µL DNA Ladder
* Well 1 : 6µL DNA Ladder
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* Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI+1µl of 6X loading dye
+
* Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye
* Gel : 1%
* Gel : 1%
|}
|}
Expect sizes :  
Expect sizes :  
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* PSB3K3 : 2750 bp
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* pSB3K3 : 2750 bp
{|
{|
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
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We can see anything. The digestion did work.
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We can't see anything. The digestion didn't work.
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|[[Team:Paris Saclay/Notebook/August/24|<big>Next day</big>]]
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|[[Team:Paris Saclay/Notebook/August/26|<big>Next day</big>]]
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{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 01:21, 5 October 2013

Contents

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Notebook : August 23

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining Pfnr, NarK, NarG or NirB and RBS-LacZ-Term or RBS-AmilCP-Term in pSB3K3

1 - Electrophoresis to check the ethanol precipitation of the digestion of BBa_J04450 by EcoRI/PstI

Psgel2308.jpg
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 5µL of BBa_J04450 digested by EcoRI/PstI +1 µl of 6X loading dye
  • Gel : 1%

Expect sizes :

  • pSB3K3 : 2750 bp

We can't see anything. The digestion didn't work.


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