Team:TU-Munich/Notebook/Materials

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==Cell lines==
-
 
+
*''E. coli'' XL-1 Blue (for molecular cloning)
-
 
+
*''E. coli'' BL21 (for cytoplasmatic protein production
 +
*''P. patens'' clone 11 obtained from Prof. Reski (Freiburg University)
==Reagents==
==Reagents==
-
<H3>Chemicals</H3>
+
==Chemicals==
-
{|cellspacing="0" border="1"
+
{|
-
|Reagent
+
|+
-
|Supplier
+
! Reagent
 +
! Supplier
 +
! Location
 +
|-
 +
| 2-Mercaptoethanol
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Acetonitrile (LC-MS grade)
 +
| Sigma-Aldrich
 +
| Taufkirchen, Germany
 +
|-
 +
| 30% Acrylamid with 0,8% Bisacrylamid
 +
| Serva Electrophoresis GmbH
 +
| Heidelberg, Germany
 +
|-
 +
| Agarose
 +
| peqlab Biotechnologie GmbH
 +
| Erlangen, Germany
 +
|-
 +
| Acetic acid (LC-MS grade)
 +
| Sigma-Aldrich Chemie GmbH
 +
| Taufkirchen
 +
|-
 +
| Ammonium acetate
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Bacto Agar Becton
 +
| BD, DIFCO Laboratories GmbH
 +
| Augsburg, Germany
 +
|-
 +
| Bacto Tryptone Becton
 +
| BD, DIFCO Laboratories GmbH
 +
| Augsburg, Germany
 +
|-
 +
| Bacto Yeast Extract Becton
 +
| BD, DIFCO Laboratories GmbH
 +
| Augsburg, Germany
 +
|-
 +
|Biotin
 +
|
 +
|
 +
|-
 +
| Boric acid
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Bromphenol blue
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Coomassie brilliant blue R-250
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Chloramphenicol
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| D(+)-Glukose, monohydrate
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| dNTPs
 +
| Invitrogen AG
 +
| Basel, Switzerland
 +
|-
 +
| Ethanol p.p., 99,9%
 +
| Honeywell Deutschland AG
 +
| Neunkirchen, Germany
 +
|-
 +
| Glycerol
 +
| Carl Roth GmbH & Co. KG
 +
| Karlsruhe, Germany
 +
|-
 +
| Kaliumchloride (KCl)
 +
| Merck KG
 +
| Darmstadt, Germany
 +
|-
 +
| Kaliumdihydrogenphosphate (KH<sub>2</sub>PO<sub>4</sub>)
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Methanol
 +
| Honeywell Deutschland AG
 +
| Neunkirchen, Germany
 +
|-
 +
| N,N,N’,N’-Tetramethylethylendiamin (TEMED)
 +
| AppliChem GmbH
 +
| Darmstadt, Germany
 +
|-
 +
| Sodiumchloride (NaCl)
 +
| Carl Roth GmbH & Co. KG
 +
| Karlsruhe, Germany
 +
|-
 +
| Sodiumdihydrogenphosphate (NaH2PO4)
 +
| Carl Roth GmbH & Co. KG
 +
| Karlsruhe, Germany
 +
|-
 +
| Disodiumhydrogenphosphate (Na2HPO4)
 +
| VWR International GmbH
 +
| Ismaning, Germany
 +
|-
 +
| Sodiumdodecylsulphate (SDS)
 +
| Carl Roth GmbH & Co. KG
 +
| Karlsruhe, Germany
 +
|-
 +
|
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|
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|
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|-
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Line 26: Line 233:
|Name
|Name
|Sequence
|Sequence
-
|
 
|-
|-
-
|
 
|EreA_for
|EreA_for
|<code>5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘</code><br>
|<code>5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘</code><br>
|-
|-
-
|
 
|EreA-rev
|EreA-rev
|<code>5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘</code><br>
|<code>5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘</code><br>
|-
|-
-
|
 
|EreA_SDMI_for
|EreA_SDMI_for
|<code>5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘</code><br>
|<code>5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘</code><br>
|-
|-
-
|
 
|EreA_SDMI_rev  
|EreA_SDMI_rev  
|<code>5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'</code><br>
|<code>5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'</code><br>
|-
|-
-
|
 
|EreA_SDMII_for
|EreA_SDMII_for
|<code>5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'</code><br>
|<code>5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'</code><br>
|-
|-
-
|
 
|EreA_SDMII_rev
|EreA_SDMII_rev
|<code>5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'</code><br>
|<code>5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'</code><br>
|-
|-
-
|
 
|EreA_SDMIII_for
|EreA_SDMIII_for
|<code>5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'</code><br>
|<code>5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'</code><br>
|-
|-
-
|
 
|EreA_SDMIII_rev
|EreA_SDMIII_rev
|<code>5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'</code><br>
|<code>5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'</code><br>
|-
|-
-
|
 
| EreB_for
| EreB_for
|<code>5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'</code><br>
|<code>5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'</code><br>
|-
|-
-
|
 
| EreB-rev  
| EreB-rev  
|<code>5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'</code><br>
|<code>5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'</code><br>
|-
|-
-
|
 
|EreB_SDMI_for
|EreB_SDMI_for
|<code>5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'</code><br>
|<code>5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'</code><br>
|-
|-
-
|
 
|EreB_SDMI_rev
|EreB_SDMI_rev
|<code>5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'</code><br>
|<code>5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'</code><br>
|-
|-
-
|
 
|EreB_SDMII_for
|EreB_SDMII_for
|<code>5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'</code><br>
|<code>5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'</code><br>
|-
|-
-
|
+
|EreB_SDMII_rev
-
| EreB_SDMII_rev
+
|<code>5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'</code><br>
|<code>5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'</code><br>
|-
|-
-
|
 
|EreB_SDMIII_for
|EreB_SDMIII_for
|<code>5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'</code><br>
|<code>5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'</code><br>
|-
|-
-
|
 
|EreB_SDMIII_rev
|EreB_SDMIII_rev
|<code>5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'</code><br>
|<code>5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'</code><br>
|-
|-
-
|
 
|TEV_fw  
|TEV_fw  
-
|<code>5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3'</code><br>
+
|<code>5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'</code><br>
|-
|-
-
|
 
|TEV_rv  
|TEV_rv  
-
|<code>5'- atataaccggtattcatgagttgagtcgcttcc - 3'</code><br>
+
|<code>5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'</code><br>
|-
|-
-
|
 
|TEV_t387c_fw  
|TEV_t387c_fw  
-
|<code>5'- atggtgtcagacactagctgcacattcccttcatc - 3'</code><br>
+
|<code>5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'</code><br>
|-
|-
-
|
 
|TEV_t387c_rv  
|TEV_t387c_rv  
-
|<code>5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3'</code><br>
+
|<code>5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'</code><br>
|-
|-
-
|
 
|N_split_TEV_fw  
|N_split_TEV_fw  
-
|<code>5'- attcgcggccgcttctag - 3'</code><br>
+
|<code>5'- ATTCGCGGCCGCTTCTAG - 3'</code><br>
|-
|-
-
|
 
|N_split_TEV_rv  
|N_split_TEV_rv  
-
|<code>5'- ttcgaaaccggtagtttggaagttggttgtcac - 3'</code><br>
+
|<code>5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'</code><br>
|-
|-
-
|
 
|C_split_TEV_fw  
|C_split_TEV_fw  
-
|<code>5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3'</code><br>
+
|<code>5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'</code><br>
|-
|-
-
|
 
|C_split_TEV_rv  
|C_split_TEV_rv  
-
|<code>5'- ccgctactagtattaaccgg - 3'</code><br>
+
|<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br>
|-
|-
-
|
 
|PIF6_2-100_fw  
|PIF6_2-100_fw  
-
|<code>5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3'</code><br>
+
|<code>5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'</code><br>
|-
|-
-
|
 
|PIF6_2-100_rv  
|PIF6_2-100_rv  
-
|<code>5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3'</code><br>
+
|<code>5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'</code><br>
|-
|-
-
|
 
|MinMCS_fo
|MinMCS_fo
-
|<code>5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3'</code><br>
+
|<code>5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
|-
|-
-
|
 
|MinMCS_rev     
|MinMCS_rev     
-
|<code>5'- gaagcttactagtcctgcaggaagcttcctaggt - 3'</code><br>
+
|<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'</code><br>
|-
|-
-
|
 
|Bpu_for
|Bpu_for
|<code>5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'</code><br>
|<code>5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'</code><br>
|-
|-
-
|
 
|Bpu_rev               
|Bpu_rev               
|<code>5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'</code><br>
|<code>5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'</code><br>
|-
|-
-
|
 
|Bpu_SDMI_for  
|Bpu_SDMI_for  
-
|<code>5'- cacttaagcattttctacgggtcgatcacaccattca - 3'</code><br>
+
|<code>5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'</code><br>
|-
|-
-
|
 
|Bpu_SDMI_rev   
|Bpu_SDMI_rev   
-
|<code>5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3'</code><br>
+
|<code>5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'</code><br>
|-
|-
-
|
 
|Bpu_SDMII_fo   
|Bpu_SDMII_fo   
-
|<code>5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3'</code><br>
+
|<code>5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'</code><br>
|-
|-
-
|
 
|Bpu_SDMII_re   
|Bpu_SDMII_re   
-
|<code>5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3'</code><br>
+
|<code>5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'</code><br>
|-
|-
-
|
 
|Bpu_SDMIII_fo
|Bpu_SDMIII_fo
-
|<code>5'- cacccttaaaaataaagctggctgcggacaggaag - 3'</code><br>
+
|<code>5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'</code><br>
|-
|-
-
|
+
|Bpu_SDMIII_re
-
|Bpu_SDMIII_re  
+
|<code>5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'</code><br>
-
|<code>5'- cttcctgtccgcagccagctttatttttaagggtg - 3'</code><br>
+
|-
|-
-
|
 
|Bpu_SDMIV_fo   
|Bpu_SDMIV_fo   
-
|<code>5'- cgtacgctcactcttacgggtacacaggataaata - 3'</code><br>
+
|<code>5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'</code><br>
|-
|-
-
|
+
|Bpu_SDMIV_re
-
|Bpu_SDMIV_re        
+
|<code>5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'</code><br>
-
|<code>5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3'</code><br>
+
|-
|-
-
|
 
|xylE_for
|xylE_for
|<code>5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG  - 3'</code><br>
|<code>5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG  - 3'</code><br>
|-         
|-         
-
|
 
|xylE_rev
|xylE_rev
|<code>5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC  - 3'</code><br>
|<code>5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC  - 3'</code><br>
|-           
|-           
-
|
 
|xylE_c312a_for
|xylE_c312a_for
|<code>5'- GAACAGTTGTGGACGGCGCGTGCGC  - 3'</code><br>
|<code>5'- GAACAGTTGTGGACGGCGCGTGCGC  - 3'</code><br>
|-
|-
-
|
 
|xylE_c312a_rev
|xylE_c312a_rev
|<code>5'- GCGCACGCGCCGTCCACAACTGTTC - 3'</code><br>
|<code>5'- GCGCACGCGCCGTCCACAACTGTTC - 3'</code><br>
|-
|-
-
|
 
|xylE_g483a_for
|xylE_g483a_for
|<code>5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'</code><br>
|<code>5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'</code><br>
|-
|-
-
|
 
|xylE_g483a_rev
|xylE_g483a_rev
|<code>5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'</code><br>
|<code>5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'</code><br>
|-
|-
-
|
 
|xylE_a834g_for
|xylE_a834g_for
|<code>5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'</code><br>
|<code>5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'</code><br>
|-
|-
-
|
 
|xylE_a834_rev
|xylE_a834_rev
|<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br>
|<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br>
|-
|-
-
|
 
|p Act_for
|p Act_for
|<code>5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'</code><br>
|<code>5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'</code><br>
|-
|-
-
|
 
|pAct_rev
|pAct_rev
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'</code><br>
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'</code><br>
|-
|-
-
|
 
|t35S_for
|t35S_for
|<code>5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'</code><br>
|<code>5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'</code><br>
|-
|-
-
|
 
|t35S_rev
|t35S_rev
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'</code><br>
|<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'</code><br>
|-
|-
-
|
 
|Npt_for
|Npt_for
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'</code><br>
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'</code><br>
|-
|-
-
|
 
|Npt_rev
|Npt_rev
|<code>5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'</code><br>
|<code>5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'</code><br>
|-
|-
-
|
 
|miniMCSII_fw
|miniMCSII_fw
|<code>5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
|<code>5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
|-
|-
-
|
 
|miniMCSII_rv
|miniMCSII_rv
|<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'</code><br>
|<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'</code><br>
|-
|-
-
|
 
|SpyTag_fw
|SpyTag_fw
|<code>5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'</code><br>
|<code>5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'</code><br>
|-
|-
-
|
 
|SpyTag_rv
|SpyTag_rv
|<code>5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'</code><br>
|<code>5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'</code><br>
|-
|-
-
|
 
|PIF3(2-100)_fw
|PIF3(2-100)_fw
|<code>5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'</code><br>
|<code>5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'</code><br>
|-
|-
-
|
 
|PIF3(2-100)_rv
|PIF3(2-100)_rv
|<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br>
|<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br>
|-
|-
-
|
 
|NucA_fw
|NucA_fw
|<code>5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'</code><br>
|<code>5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'</code><br>
|-
|-
-
|
 
|NucA_rv
|NucA_rv
|<code>5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'</code><br>
|<code>5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'</code><br>
|-
|-
-
|
 
|AlcR_fw
|AlcR_fw
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'</code><br>
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'</code><br>
|-
|-
-
|
 
|AlcR_rv
|AlcR_rv
|<code>5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'</code><br>
|<code>5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'</code><br>
|-
|-
-
|
 
|SQ_pAutoRex8_1
|SQ_pAutoRex8_1
|<code>5'- CGGGGCATCACAGCGTCG - 3'</code><br>
|<code>5'- CGGGGCATCACAGCGTCG - 3'</code><br>
|-
|-
-
|
 
|SQ_pAutoRex8_2
|SQ_pAutoRex8_2
|<code>5'- TATCGGTAACCGAGTCCG - 3'</code><br>
|<code>5'- TATCGGTAACCGAGTCCG - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCI_fw
|AlcR_QCI_fw
|<code>5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'</code><br>
|<code>5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCI_rv
|AlcR_QCI_rv
|<code>5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'</code><br>
|<code>5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCII_fw
|AlcR_QCII_fw
|<code>5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'</code><br>
|<code>5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCII_rv
|AlcR_QCII_rv
|<code>5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'</code><br>
|<code>5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCIII_fw
|AlcR_QCIII_fw
|<code>5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'</code><br>
|<code>5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCIII_rv
|AlcR_QCIII_rv
|<code>5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'</code><br>
|<code>5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCIV_fw
|AlcR_QCIV_fw
|<code>5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'</code><br>
|<code>5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'</code><br>
|-
|-
-
|
 
|AlcR_QCIV_rv
|AlcR_QCIV_rv
|<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
|<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
|-
|-
-
|
 
|pActin_QC_fw
|pActin_QC_fw
|<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
|<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
|-
|-
-
|
 
|pActin_QC_rv
|pActin_QC_rv
|<code>5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'</code><br>
|<code>5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'</code><br>
|-
|-
-
|
 
|NPT_QCI_fw
|NPT_QCI_fw
|<code>5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'</code><br>
|<code>5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'</code><br>
|-
|-
-
|
 
|NPT_QCI_rv
|NPT_QCI_rv
|<code>5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'</code><br>
|<code>5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'</code><br>
|-
|-
-
|
 
|NPT_QCII_fw
|NPT_QCII_fw
|<code>5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'</code><br>
|<code>5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'</code><br>
|-
|-
-
|
 
|NPT_QCII_rv
|NPT_QCII_rv
|<code>5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'</code><br>
|<code>5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'</code><br>
|-
|-
-
|
 
|IRES_polio_fw
|IRES_polio_fw
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'</code><br>
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'</code><br>
|-
|-
-
|
 
|IRES_polio_rv
|IRES_polio_rv
|<code>5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'</code><br>
|<code>5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'</code><br>
|-
|-
-
|
 
|BPU_SDMI_r_for
|BPU_SDMI_r_for
|<code>5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'</code><br>
|<code>5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'</code><br>
|-
|-
-
|
 
|BPU_SDMI_r_rev
|BPU_SDMI_r_rev
|<code>5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'</code><br>
|<code>5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'</code><br>
|-
|-
-
|
 
|Strep-TEV_for
|Strep-TEV_for
|<code>5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'</code><br>
|<code>5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'</code><br>
|-
|-
-
|
 
|Strep-TEV_rev
|Strep-TEV_rev
|<code>5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'</code><br>
|<code>5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'</code><br>
|-
|-
-
|
 
|SQ_pluc-Actin_1
|SQ_pluc-Actin_1
|<code>5'- CCGACTCAAATACAGATATGC - 3'</code><br>
|<code>5'- CCGACTCAAATACAGATATGC - 3'</code><br>
|-
|-
-
|
 
|SQ_pluc-Actin_2
|SQ_pluc-Actin_2
|<code>5'- CTCGGATGTAGATCTGCG - 3'</code><br>
|<code>5'- CTCGGATGTAGATCTGCG - 3'</code><br>
|-
|-
-
|
 
|pActin_QC_ins_fw
|pActin_QC_ins_fw
-
|<code>5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'</code><br>
+
|<code>5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC<br>ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'</code><br>
|-
|-
-
|
 
|pActin_QC_ins_rv
|pActin_QC_ins_rv
-
|<code>5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'</code><br>
+
|<code>5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT<br>GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'</code><br>
|-
|-
-
|
 
|pActin_Oryza_fw
|pActin_Oryza_fw
|<code>5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'</code><br>
|<code>5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'</code><br>
|-
|-
-
|
 
|pActin_Oryza_rv
|pActin_Oryza_rv
|<code>5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'</code><br>
|<code>5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'</code><br>
|-
|-
-
|
 
|GGGGSx5_TEV_fw
|GGGGSx5_TEV_fw
-
|<code>5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGGCGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'</code><br>
+
|<code>5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG<br>CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'</code><br>
|-
|-
-
|
 
|GGGGSx5_TEV_rv
|GGGGSx5_TEV_rv
-
|<code>5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCCGATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'</code><br>
+
|<code>5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC<br>GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'</code><br>
|-
|-
-
|
 
|mMCSII_fw
|mMCSII_fw
|<code>5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'</code><br>
|<code>5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'</code><br>
|-
|-
-
|
 
|mMCSII_rv
|mMCSII_rv
|<code>5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'</code><br>
|<code>5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'</code><br>
|-
|-
-
|
 
|FluA_tripmut_fw
|FluA_tripmut_fw
|<code>5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'</code><br>
|<code>5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'</code><br>
|-
|-
-
|
 
|FluA_tripmut_rv
|FluA_tripmut_rv
|<code>5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'</code><br>
|<code>5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'</code><br>
|-
|-
-
|
 
|pActin_QCI_fw
|pActin_QCI_fw
|<code>5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'</code><br>
|<code>5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'</code><br>
|-
|-
-
|
 
|pActin_QCI_rv
|pActin_QCI_rv
|<code>5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'</code><br>
|<code>5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'</code><br>
|-
|-
-
|
 
|Alc R fw Seq.
|Alc R fw Seq.
|<code>5'- CAGAGCACAATTGTCCATAC - 3'</code><br>
|<code>5'- CAGAGCACAATTGTCCATAC - 3'</code><br>
|-
|-
-
|
 
|Alc R rv Seq.
|Alc R rv Seq.
|<code>5'- TCGCGGTCGATGCTCTCC - 3'</code><br>
|<code>5'- TCGCGGTCGATGCTCTCC - 3'</code><br>
|-
|-
-
|
 
|pActin_QCins_fw
|pActin_QCins_fw
-
|<code>5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'</code><br>
+
|<code>5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC<br>CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'</code><br>
|-
|-
-
|
 
|pActin_QCins_rv
|pActin_QCins_rv
-
|<code>5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'</code><br>
+
|<code>5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG<br>TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'</code><br>
|-
|-
-
|
 
|PP1_for
|PP1_for
|<code>5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'</code><br>
|<code>5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'</code><br>
|-
|-
-
|
 
|PP1_rev
|PP1_rev
|<code>5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'</code><br>
|<code>5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'</code><br>
|-
|-
-
|
+
|PhyB fw Seq
-
|
+
|<code>5'- GCGATTTCTCAGTTACAGGC - 3'</code><br>
-
|<code>5'- - 3'</code><br>
+
|-
|-
-
|
+
|PhyB rv Seq.
-
|
+
|<code>5'- CTCAAAGCACGAGAAAGAAGC - 3'</code><br>
-
|<code>5'- - 3'</code><br>
+
|-
|-
-
|
+
|pAct_ins_fw
-
|
+
|<code>5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'</code><br>
-
|<code>5'- - 3'</code><br>
+
|-
|-
-
|
+
|pAct_ins_rv
-
|
+
|<code>5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'</code><br>
-
|<code>5'- - 3'</code><br>
+
|-
 +
|PppAct_fw
 +
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'</code><br>
 +
|-
 +
|PppAct_rv
 +
|<code>5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'</code><br>
 +
|-
 +
|PppActin_QC1_fw
 +
|<code>5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'</code><br>
 +
|-
 +
|PppActin_QC1_rv
 +
|<code>5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'</code><br>
|-
|-
-
|
+
|PppActin_QC2_fw
-
|
+
|<code>5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'</code><br>
-
|<code>5'-  - 3'</code><br>
+
|-
 +
|PppActin_QC2_rv
 +
|<code>5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'</code><br>
 +
|-
 +
|PppActin_fw_corr
 +
|<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'</code><br>
 +
|-
 +
|SYFP2_fw
 +
|<code>5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'</code><br>
 +
|-
 +
|SYFP2_rv
 +
|<code>5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'</code><br>
 +
|-
 +
|eCFP_fw
 +
|<code>5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'</code><br>
 +
|-
 +
|eCFP_rv
 +
|<code>5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'</code><br>
 +
|-
 +
|G-TEV-site-G_fw
 +
|<code>5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'</code><br>
 +
|-
 +
|G-TEV-site-G_fw
 +
|<code>5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'</code><br>
 +
|-
 +
|PppActin_seq_fw
 +
|<code>5'- CATGTCCCAGGGCCCTAG - 3'</code><br>
|}
|}
Line 477: Line 613:
-
==References:==
 
-
<div class="box-center">
 
-
<!-- Kopiervorlagen -->
 
-
[[http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984]]
 
-
#[[http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984]] Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. ''Cell'', 37(2):629–33.
 
-
<!-- Ab hier richtige Referenzen einfügen -->
 
-
#
 
-
</div>
 
-
<!-- End of content -->
 
-
 
-
{{Team:TU-Munich/TUM13_Footer}}
 
 +
<!-- Ende des Inhalts -->
</div>
</div>
</div>
</div>
-
{{Team:TU-Munich/TUM13_Script}}
+
{{Team:TU-Munich/TUM13_Footer}}
-
{{Team:TU-Munich/TUM13_Style}}
+

Latest revision as of 01:47, 5 October 2013


Cell lines

  • E. coli XL-1 Blue (for molecular cloning)
  • E. coli BL21 (for cytoplasmatic protein production
  • P. patens clone 11 obtained from Prof. Reski (Freiburg University)

Reagents

Chemicals

Reagent Supplier Location
2-Mercaptoethanol AppliChem GmbH Darmstadt, Germany
Acetonitrile (LC-MS grade) Sigma-Aldrich Taufkirchen, Germany
30% Acrylamid with 0,8% Bisacrylamid Serva Electrophoresis GmbH Heidelberg, Germany
Agarose peqlab Biotechnologie GmbH Erlangen, Germany
Acetic acid (LC-MS grade) Sigma-Aldrich Chemie GmbH Taufkirchen
Ammonium acetate AppliChem GmbH Darmstadt, Germany
Bacto Agar Becton BD, DIFCO Laboratories GmbH Augsburg, Germany
Bacto Tryptone Becton BD, DIFCO Laboratories GmbH Augsburg, Germany
Bacto Yeast Extract Becton BD, DIFCO Laboratories GmbH Augsburg, Germany
Biotin
Boric acid AppliChem GmbH Darmstadt, Germany
Bromphenol blue AppliChem GmbH Darmstadt, Germany
Coomassie brilliant blue R-250 AppliChem GmbH Darmstadt, Germany
Chloramphenicol AppliChem GmbH Darmstadt, Germany
D(+)-Glukose, monohydrate AppliChem GmbH Darmstadt, Germany
dNTPs Invitrogen AG Basel, Switzerland
Ethanol p.p., 99,9% Honeywell Deutschland AG Neunkirchen, Germany
Glycerol Carl Roth GmbH & Co. KG Karlsruhe, Germany
Kaliumchloride (KCl) Merck KG Darmstadt, Germany
Kaliumdihydrogenphosphate (KH2PO4) AppliChem GmbH Darmstadt, Germany
Methanol Honeywell Deutschland AG Neunkirchen, Germany
N,N,N’,N’-Tetramethylethylendiamin (TEMED) AppliChem GmbH Darmstadt, Germany
Sodiumchloride (NaCl) Carl Roth GmbH & Co. KG Karlsruhe, Germany
Sodiumdihydrogenphosphate (NaH2PO4) Carl Roth GmbH & Co. KG Karlsruhe, Germany
Disodiumhydrogenphosphate (Na2HPO4) VWR International GmbH Ismaning, Germany
Sodiumdodecylsulphate (SDS) Carl Roth GmbH & Co. KG Karlsruhe, Germany

Kits and Standards

Plastic Disposables

Oligonucleotides

Name Sequence
EreA_for 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘
EreA-rev 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘
EreA_SDMI_for 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘
EreA_SDMI_rev 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'
EreA_SDMII_for 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'
EreA_SDMII_rev 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'
EreA_SDMIII_for 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'
EreA_SDMIII_rev 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'
EreB_for 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'
EreB-rev 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'
EreB_SDMI_for 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'
EreB_SDMI_rev 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'
EreB_SDMII_for 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'
EreB_SDMII_rev 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'
EreB_SDMIII_for 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'
EreB_SDMIII_rev 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'
TEV_fw 5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'
TEV_rv 5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'
TEV_t387c_fw 5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'
TEV_t387c_rv 5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'
N_split_TEV_fw 5'- ATTCGCGGCCGCTTCTAG - 3'
N_split_TEV_rv 5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'
C_split_TEV_fw 5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'
C_split_TEV_rv 5'- CCGCTACTAGTATTAACCGG - 3'
PIF6_2-100_fw 5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'
PIF6_2-100_rv 5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'
MinMCS_fo 5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
MinMCS_rev 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'
Bpu_for 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'
Bpu_rev 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'
Bpu_SDMI_for 5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'
Bpu_SDMI_rev 5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'
Bpu_SDMII_fo 5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'
Bpu_SDMII_re 5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'
Bpu_SDMIII_fo 5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'
Bpu_SDMIII_re 5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'
Bpu_SDMIV_fo 5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'
Bpu_SDMIV_re 5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'
xylE_for 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'
xylE_rev 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'
xylE_c312a_for 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'
xylE_c312a_rev 5'- GCGCACGCGCCGTCCACAACTGTTC - 3'
xylE_g483a_for 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'
xylE_g483a_rev 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'
xylE_a834g_for 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'
xylE_a834_rev 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'
p Act_for 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'
pAct_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'
t35S_for 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'
t35S_rev 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'
Npt_for 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'
Npt_rev 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'
miniMCSII_fw 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'
miniMCSII_rv 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'
SpyTag_fw 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'
SpyTag_rv 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'
PIF3(2-100)_fw 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'
PIF3(2-100)_rv 5'- CCGCTACTAGTATTAACCGG - 3'
NucA_fw 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'
NucA_rv 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'
AlcR_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'
AlcR_rv 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'
SQ_pAutoRex8_1 5'- CGGGGCATCACAGCGTCG - 3'
SQ_pAutoRex8_2 5'- TATCGGTAACCGAGTCCG - 3'
AlcR_QCI_fw 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'
AlcR_QCI_rv 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'
AlcR_QCII_fw 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'
AlcR_QCII_rv 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'
AlcR_QCIII_fw 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'
AlcR_QCIII_rv 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'
AlcR_QCIV_fw 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'
AlcR_QCIV_rv 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_fw 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'
pActin_QC_rv 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'
NPT_QCI_fw 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'
NPT_QCI_rv 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'
NPT_QCII_fw 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'
NPT_QCII_rv 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'
IRES_polio_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'
IRES_polio_rv 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'
BPU_SDMI_r_for 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'
BPU_SDMI_r_rev 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'
Strep-TEV_for 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'
Strep-TEV_rev 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'
SQ_pluc-Actin_1 5'- CCGACTCAAATACAGATATGC - 3'
SQ_pluc-Actin_2 5'- CTCGGATGTAGATCTGCG - 3'
pActin_QC_ins_fw 5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC
ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'
pActin_QC_ins_rv 5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT
GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'
pActin_Oryza_fw 5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'
pActin_Oryza_rv 5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'
GGGGSx5_TEV_fw 5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG
CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
GGGGSx5_TEV_rv 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC
GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'
mMCSII_fw 5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'
mMCSII_rv 5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'
FluA_tripmut_fw 5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'
FluA_tripmut_rv 5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'
pActin_QCI_fw 5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'
pActin_QCI_rv 5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'
Alc R fw Seq. 5'- CAGAGCACAATTGTCCATAC - 3'
Alc R rv Seq. 5'- TCGCGGTCGATGCTCTCC - 3'
pActin_QCins_fw 5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC
CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'
pActin_QCins_rv 5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG
TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'
PP1_for 5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'
PP1_rev 5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'
PhyB fw Seq 5'- GCGATTTCTCAGTTACAGGC - 3'
PhyB rv Seq. 5'- CTCAAAGCACGAGAAAGAAGC - 3'
pAct_ins_fw 5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'
pAct_ins_rv 5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'
PppAct_fw 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'
PppAct_rv 5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'
PppActin_QC1_fw 5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'
PppActin_QC1_rv 5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'
PppActin_QC2_fw 5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'
PppActin_QC2_rv 5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'
PppActin_fw_corr 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'
SYFP2_fw 5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'
SYFP2_rv 5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'
eCFP_fw 5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'
eCFP_rv 5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'
G-TEV-site-G_fw 5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'
G-TEV-site-G_fw 5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'
PppActin_seq_fw 5'- CATGTCCCAGGGCCCTAG - 3'

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

Retrieved from "http://2013.igem.org/Team:TU-Munich/Notebook/Materials"