Team:Evry/Notebook/w16

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<h1>Week 16: 30th September - 6th October</h1>
<h1>Week 16: 30th September - 6th October</h1>
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<h2 align="center"> 2nd October </h2>
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<h2>Microfluidic experiment</h2>
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<p><b>30/09/13</b><br/>
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Chip's channel were full of bacteria, even after 2 hours of washing <i>(with free iron M9)</i> because we let the bacteria growth over the week-end <i>(in LB medium)</i>. Hence, we prepared another chip to launch another experiment on the next morning.<br/>
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As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.<br/>
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The first results appear promising, but we need to confirm them with a proper experiment.
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<div align="center">
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<div class="captionedPicture" style="width:40%;float:center;">
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  <a title="Bacteria expressing sfGFP, 1st experiment" href="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg">
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    <img alt="Bacteria expressing sfGFP, 1st experiment" src="https://static.igem.org/mediawiki/2013/thumb/f/fd/1st_Microfluidic_experiment.jpg/600px-1st_Microfluidic_experiment.jpg" class="Picture"/>
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  </a>
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  <div class="caption">
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    <b>Figure 1:</b> <i>Escherichia coli</i> (TOP 10 strain) expressing sfGFP.<br/>
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                    At the top of the picture, we can see that the channel is full of bacteria.
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  </div>
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  </div>
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  </div>
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<p><b id="Microflu">02/10/13</b><br/>
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<p>Our first results show that our bacteria grow well and look healthy in both M9 medium with high or low concentration of iron. Those results confirm our previous result of TECAN. However, the acquisition is not suitable for proper and further analysis. We could design a new mould for microfluidic chip that would be more suitable for our experiment.</p>
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<div align='center'><img src="https://static.igem.org/mediawiki/2013/0/06/MICROFlu.png" width="70%"/></div>
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<h2 align="center"> 4<sup>th</sup> October </h2>
<div align="center"><img src="https://static.igem.org/mediawiki/2013/9/95/WikiFreeze.jpg"/></div>
<div align="center"><img src="https://static.igem.org/mediawiki/2013/9/95/WikiFreeze.jpg"/></div>

Latest revision as of 01:50, 5 October 2013

Iron coli project

Week 16: 30th September - 6th October

Microfluidic experiment

30/09/13
Chip's channel were full of bacteria, even after 2 hours of washing (with free iron M9) because we let the bacteria growth over the week-end (in LB medium). Hence, we prepared another chip to launch another experiment on the next morning.
As our bacteria have a plasmid AceB-sfGFP and there is no iron in our M9, we still check if bacteria expressed sfGFP protein.
The first results appear promising, but we need to confirm them with a proper experiment.

Bacteria expressing sfGFP, 1st experiment
Figure 1: Escherichia coli (TOP 10 strain) expressing sfGFP.
At the top of the picture, we can see that the channel is full of bacteria.

02/10/13

Our first results show that our bacteria grow well and look healthy in both M9 medium with high or low concentration of iron. Those results confirm our previous result of TECAN. However, the acquisition is not suitable for proper and further analysis. We could design a new mould for microfluidic chip that would be more suitable for our experiment.

4th October

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