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Team:Warsaw/Glossary
From 2013.igem.org
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* '''cloning''' – on pSB1C3 of course! | * '''cloning''' – on pSB1C3 of course! | ||
* '''calm resignation''' – general mood two days prior to wiki freeze (see 'panic attack') | * '''calm resignation''' – general mood two days prior to wiki freeze (see 'panic attack') | ||
| + | *m'''Cherry''' - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry | ||
==D== | ==D== | ||
| Line 13: | Line 14: | ||
==E== | ==E== | ||
| - | * '''ethanol''' – lots of ethanol in cell lab (take a deep breath ladies and gentelmen) | + | * '''ethanol''' – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver) |
==F== | ==F== | ||
| + | * '''FedEx''' - delivery services, which tried to take our precious samples, results of our hard work | ||
* '''FluoSafe''' – our project | * '''FluoSafe''' – our project | ||
| + | |||
==G== | ==G== | ||
| + | * Airport de '''Gaulle'a''' - the place, where our parts got held up a few hours too long | ||
* '''Generacja Przyszłości (eng. ''Generation of the Future'')''' – our grant | * '''Generacja Przyszłości (eng. ''Generation of the Future'')''' – our grant | ||
| + | |||
==H== | ==H== | ||
* '''HeLa and HEK''' – cells we worked with | * '''HeLa and HEK''' – cells we worked with | ||
| Line 24: | Line 29: | ||
==I== | ==I== | ||
| - | * '''irritation''' - when this **** C- | + | * '''irritation''' - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid! |
==J== | ==J== | ||
| - | * ''' | + | * '''Jamboree''' - we're waiting for it |
==K== | ==K== | ||
| - | * '''kill-switch''' - our safety | + | * '''kill-switch''' - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC') |
==L== | ==L== | ||
* '''Lyon''' - we can send you postcard from jamboree | * '''Lyon''' - we can send you postcard from jamboree | ||
| + | * '''lysis device''' - another component of our kill-switch (see: 'MinC') | ||
==M== | ==M== | ||
| - | * '''MinC''' - core of our kill-switch | + | * '''MinC''' - core of our kill-switch. Inhibits bacterial division. |
==N== | ==N== | ||
| + | *'''N-terminal valine''' - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor. | ||
| + | |||
==O== | ==O== | ||
| + | *m'''Orange''' - we nearly managed to produce BiFC fragments form this fluorescent protein | ||
| + | |||
==P== | ==P== | ||
* '''PCR''' – polymerase chain reaction | * '''PCR''' – polymerase chain reaction | ||
| + | * '''panic attack''' – begins approximately 12 h prior to wiki freeze | ||
| + | |||
==Q== | ==Q== | ||
| + | *'''questionable''' - large portion of our clones and PCR products | ||
| + | |||
==R== | ==R== | ||
* '''roGFP''' - redox-sensitive GFP | * '''roGFP''' - redox-sensitive GFP | ||
==S== | ==S== | ||
| - | * '''superfolder proteins''' | + | * '''superfolder proteins''' - improved derivatives of ''A. victoria''-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over) |
| + | |||
==T== | ==T== | ||
* '''Toolbox (BiFC's)''' - one of our goals | * '''Toolbox (BiFC's)''' - one of our goals | ||
| Line 53: | Line 68: | ||
==U== | ==U== | ||
* '''ultraviolet''' - and GFP glows | * '''ultraviolet''' - and GFP glows | ||
| + | * '''The Unknown Sample''' - not all abbreviations are as obvious now as there were a month ago... | ||
==V== | ==V== | ||
| Line 59: | Line 75: | ||
==W== | ==W== | ||
* '''wirówka (eng. ''centrifuge'')''' – that we unintentionally broke! | * '''wirówka (eng. ''centrifuge'')''' – that we unintentionally broke! | ||
| + | * '''wiki freeze''' – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack') | ||
==X== | ==X== | ||
| + | *'''X-Files''' - paranormal no longer seems impressive, once you've seen some of our results... | ||
| + | |||
==Y== | ==Y== | ||
* '''yellow fluorescent proteins (sfYFP and SYFP2)''' - and their glowing rivalry! | * '''yellow fluorescent proteins (sfYFP and SYFP2)''' - and their glowing rivalry! | ||
==Z== | ==Z== | ||
| + | *'''Zombie walk''' - team departs the lab following wiki freeze | ||
{{:Team:Warsaw/Templates/StandardPageEnd}} | {{:Team:Warsaw/Templates/StandardPageEnd}} | ||
Latest revision as of 03:46, 5 October 2013
Project description
Contents |
A
- acrylamide – our subject of study
B
- BiFC – Bimolecular Fluorescent Complementation
C
- cloning – on pSB1C3 of course!
- calm resignation – general mood two days prior to wiki freeze (see 'panic attack')
- mCherry - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry
D
- despair - general mood week prior to wiki freeze (see 'calm resignation')
E
- ethanol – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver)
F
- FedEx - delivery services, which tried to take our precious samples, results of our hard work
- FluoSafe – our project
G
- Airport de Gaulle'a - the place, where our parts got held up a few hours too long
- Generacja Przyszłości (eng. Generation of the Future) – our grant
H
- HeLa and HEK – cells we worked with
- hemoglobin - core of our sensor
I
- irritation - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid!
J
- Jamboree - we're waiting for it
K
- kill-switch - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC')
L
- Lyon - we can send you postcard from jamboree
- lysis device - another component of our kill-switch (see: 'MinC')
M
- MinC - core of our kill-switch. Inhibits bacterial division.
N
- N-terminal valine - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor.
O
- mOrange - we nearly managed to produce BiFC fragments form this fluorescent protein
P
- PCR – polymerase chain reaction
- panic attack – begins approximately 12 h prior to wiki freeze
Q
- questionable - large portion of our clones and PCR products
R
- roGFP - redox-sensitive GFP
S
- superfolder proteins - improved derivatives of A. victoria-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over)
T
- Toolbox (BiFC's) - one of our goals
U
- ultraviolet - and GFP glows
- The Unknown Sample - not all abbreviations are as obvious now as there were a month ago...
V
- vacation - in lab all the time!
W
- wirówka (eng. centrifuge) – that we unintentionally broke!
- wiki freeze – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack')
X
- X-Files - paranormal no longer seems impressive, once you've seen some of our results...
Y
- yellow fluorescent proteins (sfYFP and SYFP2) - and their glowing rivalry!
Z
- Zombie walk - team departs the lab following wiki freeze
