Team:Warsaw/Glossary
From 2013.igem.org
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+ | * '''FedEx''' - delivery services, which tried to take our precious samples, results of our hard work | ||
* '''FluoSafe''' – our project | * '''FluoSafe''' – our project | ||
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==G== | ==G== | ||
+ | * Airport de '''Gaulle'a''' - the place, where our parts got held up a few hours too long | ||
* '''Generacja Przyszłości (eng. ''Generation of the Future'')''' – our grant | * '''Generacja Przyszłości (eng. ''Generation of the Future'')''' – our grant | ||
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==H== | ==H== | ||
* '''HeLa and HEK''' – cells we worked with | * '''HeLa and HEK''' – cells we worked with | ||
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==U== | ==U== | ||
* '''ultraviolet''' - and GFP glows | * '''ultraviolet''' - and GFP glows | ||
+ | * '''The Unknown Sample''' - not all abbreviations are as obvious now as there were a month ago... | ||
==V== | ==V== |
Latest revision as of 03:46, 5 October 2013
Project description
Contents |
A
- acrylamide – our subject of study
B
- BiFC – Bimolecular Fluorescent Complementation
C
- cloning – on pSB1C3 of course!
- calm resignation – general mood two days prior to wiki freeze (see 'panic attack')
- mCherry - red fluorescent protein. We managed to succesfully clone C- and N-terminal BiFC fragments into pSB1C3 and deliver them to the Registry
D
- despair - general mood week prior to wiki freeze (see 'calm resignation')
E
- ethanol – lots of ethanol in cell lab (take a deep breath ladies and gentelmen - unless one of you is the designated driver)
F
- FedEx - delivery services, which tried to take our precious samples, results of our hard work
- FluoSafe – our project
G
- Airport de Gaulle'a - the place, where our parts got held up a few hours too long
- Generacja Przyszłości (eng. Generation of the Future) – our grant
H
- HeLa and HEK – cells we worked with
- hemoglobin - core of our sensor
I
- irritation - when this **** C-m6 and C-m12 cannot clone in pSB1C3 plasmid!
J
- Jamboree - we're waiting for it
K
- kill-switch - our safety system which wasn't synthetized on time (see: 'lysis device' and 'MinC')
L
- Lyon - we can send you postcard from jamboree
- lysis device - another component of our kill-switch (see: 'MinC')
M
- MinC - core of our kill-switch. Inhibits bacterial division.
N
- N-terminal valine - acrylamide forms adducts on the N-terminus of hemoglobin alpha and beta chains. Adducts interfere with tetramer formation. We attempted to take advantage of this property while designing the hemoglobin-based acrylamide sensor.
O
- mOrange - we nearly managed to produce BiFC fragments form this fluorescent protein
P
- PCR – polymerase chain reaction
- panic attack – begins approximately 12 h prior to wiki freeze
Q
- questionable - large portion of our clones and PCR products
R
- roGFP - redox-sensitive GFP
S
- superfolder proteins - improved derivatives of A. victoria-based fluorescent proteins (green, yellow, blue and cyan). This part of our project actually succeded. They're brighter, fun to work with and considerably more stable (as a poet once said: 'harder, better, faster, stronger' - and our work with them still isn't over)
T
- Toolbox (BiFC's) - one of our goals
U
- ultraviolet - and GFP glows
- The Unknown Sample - not all abbreviations are as obvious now as there were a month ago...
V
- vacation - in lab all the time!
W
- wirówka (eng. centrifuge) – that we unintentionally broke!
- wiki freeze – the ultimate deadline (see: "despair', 'calm resignation' and 'panic attack')
X
- X-Files - paranormal no longer seems impressive, once you've seen some of our results...
Y
- yellow fluorescent proteins (sfYFP and SYFP2) - and their glowing rivalry!
Z
- Zombie walk - team departs the lab following wiki freeze