Team:ATOMS-Turkiye/deneme

From 2013.igem.org

(Difference between revisions)
 
(27 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:ATOMS-Turkiye/Templates/Style}}
{{:Team:ATOMS-Turkiye/Templates/Style}}
{{:Team:ATOMS-Turkiye/Templates/Head}}
{{:Team:ATOMS-Turkiye/Templates/Head}}
-
{{:Team:ATOMS-Turkiye/ruibillboard.js}}
 
<html>
<html>
Line 10: Line 9:
       item = $("#slideshow items").children("div").eq(pg-1);
       item = $("#slideshow items").children("div").eq(pg-1);
       content=$("#slideshow #right");
       content=$("#slideshow #right");
 +
      content.stop();
       content.fadeOut(200);
       content.fadeOut(200);
       content.html(item.find("#text").html());
       content.html(item.find("#text").html());
Line 16: Line 16:
       image.css("position","relative");
       image.css("position","relative");
       imagebox = $("#slideshow").find( "#imagebox");
       imagebox = $("#slideshow").find( "#imagebox");
 +
      imagebox.stop(200);
       imagebox.fadeOut(200);
       imagebox.fadeOut(200);
       //image.width(imagebox.width());
       //image.width(imagebox.width());
-
       image.height(imagebox.height()-$("#pager").height());
+
       image.height(imagebox.height());
       imagebox.html(image.clone());
       imagebox.html(image.clone());
       imagebox.fadeIn(200);
       imagebox.fadeIn(200);
Line 29: Line 30:
       for( i=1;i<=l;i++ )
       for( i=1;i<=l;i++ )
         pages+="<li onclick='openPage("+i+");' ><a href='#' class='no-action'>"+i+"</a></li> ";
         pages+="<li onclick='openPage("+i+");' ><a href='#' class='no-action'>"+i+"</a></li> ";
 +
      pages+="</ul>"
 +
      pages = $(pages);
 +
      pages.children("li").hover(function(){openPage(parseInt($(this).text()));});
       pager.html(pages);
       pager.html(pages);
       openPage(1);
       openPage(1);
Line 37: Line 41:
   a.find(".rui-billboard-button-prev").click(function(e){
   a.find(".rui-billboard-button-prev").click(function(e){
     b= a.find(".rui-billboard-current");
     b= a.find(".rui-billboard-current");
-
     b.removeClass("rui-billboard-current");
+
     if(b.is(':animated'))
 +
      return;
     b.css("left",0);
     b.css("left",0);
     b.animate({
     b.animate({
-
         left: b.outerWidth()
+
         left: a.outerWidth(true)
-
     });
+
     },function(){$(this).removeClass("rui-billboard-current");});
     b=b.prev("li");
     b=b.prev("li");
     if(b.length<1)
     if(b.length<1)
     b=a.find("li").last();
     b=a.find("li").last();
     b.addClass("rui-billboard-current");
     b.addClass("rui-billboard-current");
-
     b.css("left",-b.outerWidth());
+
     b.css("left",-a.outerWidth(true));
     b.animate({
     b.animate({
         left: 0
         left: 0
Line 53: Line 58:
   a.find(".rui-billboard-button-next").click(function(e){
   a.find(".rui-billboard-button-next").click(function(e){
     b= a.find(".rui-billboard-current");
     b= a.find(".rui-billboard-current");
 +
    if(b.is(':animated'))
 +
      return;
     b.stop();
     b.stop();
     b.css("left",0);
     b.css("left",0);
     b.animate({
     b.animate({
-
         left: -b.outerWidth()
+
         left: -a.outerWidth(true)
-
     });
+
     },function(){$(this).removeClass("rui-billboard-current");});
-
     b.removeClass("rui-billboard-current");
+
     //b.removeClass("rui-billboard-current");
     b=b.next("li");
     b=b.next("li");
     if(b.length<1)
     if(b.length<1)
     b=a.find("li").first();
     b=a.find("li").first();
 +
    b.stop();
     b.addClass("rui-billboard-current");
     b.addClass("rui-billboard-current");
-
    b.stop();
+
     b.css("left",a.outerWidth(true));
-
     b.css("left",b.outerWidth());
+
     b.animate({
     b.animate({
     left: 0
     left: 0
Line 83: Line 90:
           <image id="image" src="https://static.igem.org/mediawiki/2013/6/66/Oncoli-diagram-2.jpg"  />
           <image id="image" src="https://static.igem.org/mediawiki/2013/6/66/Oncoli-diagram-2.jpg"  />
           <div id="text">
           <div id="text">
-
             <p class='infocard'>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Morbi quis diam et felis adipiscing molestie. Nam elit odio, ullamcorper quis vehicula quis, fermentum quis urna. Pellentesque ultrices, ante quis adipiscing consectetur, massa velit tempor est, vel condimentum odio turpis cursus risus. Mauris imperdiet elit ligula, quis sodales nulla elementum eget. Nulla in vestibulum orci, vitae blandit massa. Maecenas ac tellus sit amet sapien tincidunt posuere quis quis elit. Cras at tristique justo. Suspendisse sit amet tortor ultrices, accumsan magna et, molestie felis. Donec a enim nibh. Quisque id dolor semper, tempor purus consequat, dignissim turpis. Phasellus condimentum massa interdum porttitor mattis.</p>
+
             <p class='infocard'><b>Welcome to our model of Oncoli.</b><br/> The genetically engineered Nissle 1917 bacteria present in the lumen produce nanofactories which are responsible of recognizing and inducing the apoptosis mechanism of cancer cells.</p>
           </div>
           </div>
         </div>
         </div>
Line 89: Line 96:
           <image id="image" src="https://static.igem.org/mediawiki/2013/c/cb/Oncoli-diagram-3.jpg" />
           <image id="image" src="https://static.igem.org/mediawiki/2013/c/cb/Oncoli-diagram-3.jpg" />
           <div id="text">
           <div id="text">
-
             <p class='infocard'>Curabitur malesuada vulputate mi, non fermentum justo vestibulum eu. Quisque semper nulla erat, quis tristique massa gravida at. In hac habitasse platea dictumst. Nunc gravida ut ipsum at dapibus. Nunc scelerisque velit in mauris venenatis, id consequat nisl aliquam. Nunc fringilla mauris non mauris luctus dictum. Etiam vulputate posuere velit non fermentum. Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas. Quisque sodales leo quam, eget condimentum ipsum sagittis in. Duis molestie, lectus vitae consectetur rhoncus, orci orci iaculis massa, et bibendum magna felis nec enim. Mauris vel arcu elementum, sagittis lorem in, malesuada magna. Mauris tincidunt vehicula luctus. Maecenas cursus semper diam.</p>
+
             <p class='infocard'>The nanofactory complex produced by our bacteria is formed by three parts. These are: Anti-EpCAM , Protein G and our enzymes Luxs-pfs and His-link-enzyme. These nanofactories are released from bacteria into the lumen via signal peptides. Once the Anti-EpCAM’s present on the nanofactories bind to the EpCAM antigens present on the surface of epithelial cells, the enzymes Luxs-pfs and His-link-enzyme present within the nanofactories begin to use SAH in the lumen to produce AI-2: Quorum sensing material. </p>
           </div>
           </div>
         </div>
         </div>
Line 95: Line 102:
           <image id="image" src="https://static.igem.org/mediawiki/2013/2/26/Oncoli-diagram-4.jpg" />
           <image id="image" src="https://static.igem.org/mediawiki/2013/2/26/Oncoli-diagram-4.jpg" />
           <div id="text">
           <div id="text">
-
             <p class='infocard'></p>
+
             <p class='infocard'>Compared to normal cells, EpCAM antigens are expressed 100 times more on a cancer cell. Therefore the concentration of bound nanofactories on a cancer cell is 100 times greater.</p>
           </div>
           </div>
         </div>
         </div>
Line 101: Line 108:
           <image id="image" src="https://static.igem.org/mediawiki/2013/5/52/Oncoli-diagram-6.jpg" />
           <image id="image" src="https://static.igem.org/mediawiki/2013/5/52/Oncoli-diagram-6.jpg" />
           <div id="text">
           <div id="text">
-
             <p class='infocard'></p>
+
             <p class='infocard'>The AI-2 substance produced in the lumen by our nanofactories act like a chemo-attractant which trigger our Nissle 1917 bacteria to motions toward the cancer cells via the quorum sensing technique.</p>
           </div>
           </div>
         </div>
         </div>
Line 107: Line 114:
           <image id="image" src="https://static.igem.org/mediawiki/2013/b/b2/Oncoli-diagram-7.jpg" />
           <image id="image" src="https://static.igem.org/mediawiki/2013/b/b2/Oncoli-diagram-7.jpg" />
           <div id="text">
           <div id="text">
-
             <p class='infocard'></p>
+
             <p class='infocard'>TAT-Apoptin: our killer protein is controlled by an inducible promoter called LsrR-lsrk. LsrR-lsrk is induced by AI-2 which is formed when our nanofactories bind to the cancer cells. Our bacteria build up around cancer cells and release our killer protein: TAT-Apoptin. </p>
           </div>
           </div>
         </div>
         </div>
Line 113: Line 120:
           <image id="image" src="https://static.igem.org/mediawiki/2013/5/58/Oncoli-diagram-11.jpg" />
           <image id="image" src="https://static.igem.org/mediawiki/2013/5/58/Oncoli-diagram-11.jpg" />
           <div id="text">
           <div id="text">
-
             <p class='infocard'></p>
+
             <p class='infocard'>The secreted TAT-APOPTIN complex, binds to the cancer cells and normal cells, penetrating into the membrane without causing any damage. When apoptin reaches a specific concentration in the cytoplasm of cancer cells, the period of apoptosis begins and the cancer cells are destroyed. However the normal cells remain unaffected as apoptin is cancer specific. </p>
           </div>
           </div>
         </div>
         </div>
       </items>
       </items>
-
       <div id="left" style="position:relative;float:left;width:50%;height:100%;">
+
       <div id="left" style="position:relative;float:left;width:57.7%;height:100%;">
         <div id="imagebox" style="position:relative;text-align:center;width:100%;height:100%;overflow:hidden;">
         <div id="imagebox" style="position:relative;text-align:center;width:100%;height:100%;overflow:hidden;">
           image here
           image here
Line 125: Line 132:
       </div>
       </div>
        
        
-
       <div class="boxin" style="float:left;width:50%;height:100%;background:#dfdfdf;overflow:hidden;">
+
       <div class="boxin" style="float:left;width:42.3%;height:100%;overflow:hidden;background: #233447;">
         <div id="right" style="width:100%;height:100%;">
         <div id="right" style="width:100%;height:100%;">
           content here
           content here
Line 133: Line 140:
   </div>
   </div>
    
    
-
  <ul class="quicklinks box">
+
  <div class="tourview" style="background:url('https://static.igem.org/mediawiki/2013/thumb/e/e3/Oncoli-tour-main.png/800px-Oncoli-tour-main.png');-webkit-background-size: cover;-moz-background-size: cover;-o-background-size: cover;background-size: cover;">
-
   <li><a href="/Team:ATOMS-Turkiye/Project">Module 1</a></li>
+
   <div style="padding-left:130px;padding-right:170px;padding-left:130px;padding-top:100px;padding-bottom:55px;height:135px;">
-
  <li><a href="/Team:ATOMS-Turkiye/Project">Module 2</a></li>
+
  <a href="/Team:ATOMS-Turkiye/Tour:1" title="Take a tour"></a>
-
  <li><a href="/Team:ATOMS-Turkiye/Project">Module 3</a></li>
+
  <a href="/Team:ATOMS-Turkiye/Tour:2"></a>
-
</ul>
+
  <a href="/Team:ATOMS-Turkiye/Tour:3"></a>
-
<ul id="billboard" class="rui-billboard">
+
  <a href="/Team:ATOMS-Turkiye/Tour:4"></a>
-
  <li>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Integer pretium viverra ante, eu condimentum est ultricies quis. Sed ut bibendum lorem. Duis sem arcu, egestas vitae rutrum quis, vestibulum non velit. Cras velit metus, sagittis et pharetra a, viverra sed libero. Donec convallis erat vel massa vehicula venenatis. Cras auctor, quam ac blandit rhoncus, erat ante pellentesque risus, at molestie ante tortor in lorem. In porta quam et mollis lobortis. Integer id purus nunc. Donec faucibus velit sem, eget venenatis leo vulputate ut. Quisque sed aliquam eros, in rhoncus ligula. Donec eleifend augue quis magna consectetur, eget tempus neque adipiscing.</li>
+
   </div>
-
  <li>Cras blandit, leo eget aliquet sodales, elit enim mollis nisl, ut posuere elit dui ac nisl. Suspendisse ante quam, pretium id enim ac, posuere pulvinar libero. Nullam vel fermentum est. Nunc vestibulum vulputate dolor, a dictum justo ultricies pellentesque. Vestibulum at erat dolor. Donec quis gravida turpis. Mauris nisl ante, suscipit sit amet justo dictum, lobortis condimentum mi. Maecenas lacinia sapien augue, nec iaculis nulla vehicula vel. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Quisque sit amet placerat metus. Curabitur sed fermentum neque, et convallis risus. Cras id libero enim.</li>
+
</div>
-
   <li>In hac habitasse platea dictumst. Aenean imperdiet, metus nec luctus venenatis, nisi sapien sodales leo, molestie congue justo augue ut nunc. Nam ac semper elit. Donec rhoncus nunc ut dolor condimentum, vel pellentesque orci gravida. Aliquam dictum blandit sem, rhoncus tristique est bibendum ut. Aliquam ut laoreet nibh. Nulla quis eros a justo volutpat tempor ac in metus. Curabitur cursus ac elit vel malesuada. Duis volutpat tortor vitae ornare sagittis. Morbi at dolor at metus viverra fermentum sit amet vitae augue</li>
+
 
-
  <div class="rui-billboard-button-prev">&lsaquo;</div>
+
 
-
  <div class="rui-billboard-button-next">&rsaquo;</div>
+
   
-
  </ul>
+
 
</html>
</html>
 +
 +
{{:Team:ATOMS-Turkiye/Sponsors}}

Latest revision as of 03:48, 5 October 2013

Welcome to our model of Oncoli.
The genetically engineered Nissle 1917 bacteria present in the lumen produce nanofactories which are responsible of recognizing and inducing the apoptosis mechanism of cancer cells.

The nanofactory complex produced by our bacteria is formed by three parts. These are: Anti-EpCAM , Protein G and our enzymes Luxs-pfs and His-link-enzyme. These nanofactories are released from bacteria into the lumen via signal peptides. Once the Anti-EpCAM’s present on the nanofactories bind to the EpCAM antigens present on the surface of epithelial cells, the enzymes Luxs-pfs and His-link-enzyme present within the nanofactories begin to use SAH in the lumen to produce AI-2: Quorum sensing material.

Compared to normal cells, EpCAM antigens are expressed 100 times more on a cancer cell. Therefore the concentration of bound nanofactories on a cancer cell is 100 times greater.

The AI-2 substance produced in the lumen by our nanofactories act like a chemo-attractant which trigger our Nissle 1917 bacteria to motions toward the cancer cells via the quorum sensing technique.

TAT-Apoptin: our killer protein is controlled by an inducible promoter called LsrR-lsrk. LsrR-lsrk is induced by AI-2 which is formed when our nanofactories bind to the cancer cells. Our bacteria build up around cancer cells and release our killer protein: TAT-Apoptin.

The secreted TAT-APOPTIN complex, binds to the cancer cells and normal cells, penetrating into the membrane without causing any damage. When apoptin reaches a specific concentration in the cytoplasm of cancer cells, the period of apoptosis begins and the cancer cells are destroyed. However the normal cells remain unaffected as apoptin is cancer specific.

image here

Sponsors

Oncoli-s-tozal.png Oncoli-s-sigma.jpg Oncoli-s-sentegen.png

Retrieved from "http://2013.igem.org/Team:ATOMS-Turkiye/deneme"