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Team:Uppsala/toxin-antitoxin-system
From 2013.igem.org
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<li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a> | <li><a href="https://2013.igem.org/Team:Uppsala/metabolic-engineering">Metabolic engineering</a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid"> | + | <li><a href="https://2013.igem.org/Team:Uppsala/p-coumaric-acid">p-Coumaric acid</a></li> |
<li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/resveratrol">Resveratrol</a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/lycopene">Lycopene</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a> | <li><a href="https://2013.igem.org/Team:Uppsala/modeling" id="list_type1"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/6/63/Uppsala2013_Modeling.png"></a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway"> | + | <li><a href="https://2013.igem.org/Team:Uppsala/P-Coumaric-acid-pathway">Kinetic model</a></li> |
<li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/modeling-tutorial">Modeling tutorial </a></li> | ||
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| + | <li><a href="https://2013.igem.org/Team:Uppsala/toxicity-model">Toxicity model</a></li> | ||
</ul></li> | </ul></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/parts" id="list_type2"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/e/eb/Uppsala2013_parts.png"></a></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/carotenoid-group">Carotenoid group</a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/chassi-group">Chassi group</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/advisors">Advisors</a></li> | ||
</ul></li> | </ul></li> | ||
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<li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/public-opinion">Public opinion </a></li> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/Outreach">High school & media </a></li> | ||
| - | + | <li><a href="https://2013.igem.org/Team:Uppsala/bioart">BioArt</a></li> | |
| + | <li><a href="https://2013.igem.org/Team:Uppsala/LactonutritiousWorld">A LactoWorld</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/killswitches">Killswitches</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/realization">Patent</a></li> | ||
</ul></li> | </ul></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a | + | <li><a href="https://2013.igem.org/Team:Uppsala/attribution" id="list_type4"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/5/5d/Uppsala2013_Attributions.png"></a></li> |
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<li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a> | <li><a href="https://2013.igem.org/Team:Uppsala/notebook" id="list_type3"><img class="nav-text" src="https://static.igem.org/mediawiki/2013/3/36/Uppsala2013_Notebook.png"></a> | ||
<ul> | <ul> | ||
<li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li> | <li><a href="https://2013.igem.org/Team:Uppsala/safety-form">Safety form</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Uppsala/protocols">Protocols</a></li> | ||
</ul></li> | </ul></li> | ||
</ul> | </ul> | ||
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One of the challenges when creating synthetic systems in bacteria that serve a purpose besides increasing the fitness of the organism is that there is a negative selective pressure against keeping the system. Toxin-antitoxin systems can be used to make plasmids far more stabile without having to use antibiotics and antibiotic resistance. If a clone were to lose the plasmid, the toxin which usually has a longer half life than the antitoxin will kill the bacteria. | One of the challenges when creating synthetic systems in bacteria that serve a purpose besides increasing the fitness of the organism is that there is a negative selective pressure against keeping the system. Toxin-antitoxin systems can be used to make plasmids far more stabile without having to use antibiotics and antibiotic resistance. If a clone were to lose the plasmid, the toxin which usually has a longer half life than the antitoxin will kill the bacteria. | ||
| - | <img class="method-plasmid" src="https://static.igem.org/mediawiki/2013/d/dc/Uppsala2013_anti-toxin-toxin-system.jpg"> | + | <a href="https://static.igem.org/mediawiki/2013/d/dc/Uppsala2013_anti-toxin-toxin-system.jpg" data-lightbox="roadtrip"><img class="method-plasmid" src="https://static.igem.org/mediawiki/2013/d/dc/Uppsala2013_anti-toxin-toxin-system.jpg"></a> |
<p>Above is an example of how a toxin-antitoxin system could be applied together with a gene X. If the gene is toxic or expressed strongly enough there will be a substantial evolutionary pressure to lose the plasmid during cell division. However if the gene is present on a plasmid with a toxin-antitoxin system would be lethal due to the loss of the antitoxin gene.</p> | <p>Above is an example of how a toxin-antitoxin system could be applied together with a gene X. If the gene is toxic or expressed strongly enough there will be a substantial evolutionary pressure to lose the plasmid during cell division. However if the gene is present on a plasmid with a toxin-antitoxin system would be lethal due to the loss of the antitoxin gene.</p> | ||
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<li> <a href="http://parts.igem.org/Part:BBa_K1033260">BBa_K1033260</a> - antitoxin system from Lactobacillus plantarum Toxin<br> </li> | <li> <a href="http://parts.igem.org/Part:BBa_K1033260">BBa_K1033260</a> - antitoxin system from Lactobacillus plantarum Toxin<br> </li> | ||
| - | <h1> References: </h1> <a id="refpoint" | + | <h1> References: </h1> <a id="refpoint"> |
| - | [1] Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, <a href="http://mic.sgmjournals.org/content/151/2/421.long"> Elisabeth Sorvig et al. September 30 2004 </a> | + | [1] </a> Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, <a href="http://mic.sgmjournals.org/content/151/2/421.long"> Elisabeth Sorvig et al. September 30 2004 </a> |
Latest revision as of 21:28, 28 October 2013
Toxin-antitoxin system
Keep your plasmids without antibiotic resistance
One of the challenges when creating synthetic systems in bacteria that serve a purpose besides increasing the fitness of the organism is that there is a negative selective pressure against keeping the system. Toxin-antitoxin systems can be used to make plasmids far more stabile without having to use antibiotics and antibiotic resistance. If a clone were to lose the plasmid, the toxin which usually has a longer half life than the antitoxin will kill the bacteria.
Above is an example of how a toxin-antitoxin system could be applied together with a gene X. If the gene is toxic or expressed strongly enough there will be a substantial evolutionary pressure to lose the plasmid during cell division. However if the gene is present on a plasmid with a toxin-antitoxin system would be lethal due to the loss of the antitoxin gene.
