Team:British Columbia/Notebook/Protocols
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{{:Team:British_Columbia/Templates/MainHeader}} | {{:Team:British_Columbia/Templates/MainHeader}} | ||
- | ==Protocols | + | <html> |
- | ===[[Team:British_Columbia/Notebook/Protocols/ | + | <a name="top"></a> |
+ | </html> | ||
+ | =Protocols= | ||
+ | ===[[Team:British_Columbia/Notebook/Protocols/PCR|PCR]]=== | ||
Instruction for colony PCR and regular PCR | Instruction for colony PCR and regular PCR | ||
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+ | ===[[Team:British_Columbia/Notebook/Protocols/gelverification|Gel Verification]]=== | ||
+ | Make a gel and conduct gel elecrophoresis | ||
+ | |||
+ | ===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]=== | ||
+ | Restriction digest with BioBrick plasmid an inserts | ||
+ | |||
+ | ===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]=== | ||
+ | Ligate Biobrick insert to vector | ||
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+ | ===[[Team:British_Columbia/Notebook/Protocols/plates|Making Plates]]=== | ||
+ | Make LB plates with antibiotics | ||
+ | |||
+ | ===[[Team:British_Columbia/Notebook/Protocols/media|Liquid media]]=== | ||
+ | Make liquid LB media | ||
+ | |||
+ | ===[[Team:British_Columbia/Notebook/Protocols/compcells|Competent cells]]=== | ||
+ | Preparation of chemically competent cells | ||
+ | |||
+ | ===[[Team:British_Columbia/Notebook/Protocols/transformation|Transformation]]=== | ||
+ | Transformation of plasmi DNA into chemically competent cells | ||
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]=== | ||
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===[[Team:British_Columbia/Notebook/Protocols/GCMS|Gas chromatography-mass spectrometry for biosynthetic product detection]]=== | ===[[Team:British_Columbia/Notebook/Protocols/GCMS|Gas chromatography-mass spectrometry for biosynthetic product detection]]=== | ||
- | GC-MS Protocol for assaying biosynthetic product production | + | GC-MS Protocol for assaying biosynthetic product production |
===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]=== | ===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]=== | ||
Make combinatorial part libraries from compatible parts | Make combinatorial part libraries from compatible parts | ||
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+ | <br> | ||
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+ | <html> | ||
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+ | <div> | ||
+ | <center> | ||
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+ | <a href="https://2013.igem.org/Team:British_Columbia/Notebook"> | ||
+ | <img width="180" class="icon" src="https://static.igem.org/mediawiki/2013/7/79/UBCReturnArrow.png" | ||
+ | onmouseover="this.src='https://static.igem.org/mediawiki/2013/8/8a/UBCReturnNotebook2.png'" | ||
+ | onmouseout="this.src='https://static.igem.org/mediawiki/2013/7/79/UBCReturnArrow.png'"/></a> | ||
+ | </center> | ||
+ | </div> | ||
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+ | </html> | ||
+ | <html> | ||
+ | <div align="right"><a href="#top">Top of Page</a></div> | ||
+ | </html> |
Latest revision as of 03:47, 29 October 2013
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Protocols
PCR
Instruction for colony PCR and regular PCR
Gel Verification
Make a gel and conduct gel elecrophoresis
BioBrick Restriction Digest
Restriction digest with BioBrick plasmid an inserts
BioBrick Ligation
Ligate Biobrick insert to vector
Making Plates
Make LB plates with antibiotics
Liquid media
Make liquid LB media
Competent cells
Preparation of chemically competent cells
Transformation
Transformation of plasmi DNA into chemically competent cells
CRISPR Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
Electrocompetent Cell Production
Gas chromatography-mass spectrometry for biosynthetic product detection
GC-MS Protocol for assaying biosynthetic product production
Make Combinatorial Part Libraries
Make combinatorial part libraries from compatible parts